High-efficient nitrogen removal by coupling enriched autotrophic-nitrification and aerobic-denitrification consortiums at cold temperature

The effects of culture conditions on a newly isolated Pseudomonas stutzeri KTB's ability to simultaneously perform heterotrophic nitrification and aerobic denitrification were investigated to determine its potential of application in nitrogen removal from wastewater. The results from experiments in the presence of 10 mmol/L of ammonium were as follows: succinate was the preferred carbon source, and the optimum C/N ratio, temperature, and initial pH were 10, 30 °C, and 7–8, respectively. Nitrogen removal took place not only in the logarithmic phase but also in the stationary phase. Under the optimum conditions, the nitrogen removal rate increased as the ammonium concentration elevated, until it was as high as 60 mmol/L. Meanwhile, the maximum specific growth rate decreased. The highest nitrogen removal rate of 0.977 mmol/L/h was observed at 60 mmol/L of ammonium and the maximum removal ratio of 85.6% at 40 mmol/L when the bacterial treatment for 48 h was completed. The strain was vulnerable to even higher ammonium loads. When incubated in anaerobically digested hennery wastewater containing 43.85 mmol/L of ammonium and 2.32 mmol/L of nitrate, the removal ratio and rate reached 82.4% and 0.397 mmol/L/h, respectively. The strain might be a great candidate for ammonium removal from wastewater.

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Phosphorus and nitrogen removal by a novel phosphate-accumulating organism, Arthrobacter sp. HHEP5 capable of heterotrophic nitrification-aerobic denitrification: Safety assessment, removal characterization, mechanism exploration and wastewater treatment

Bioresource Technology ◽

10.1016/j.biortech.2020.123633 ◽

2020 ◽

Vol 312 ◽

pp. 123633 ◽

Cited By ~ 3

Author(s):

Mengyu Zhang ◽

Luqing Pan ◽

Liping Liu ◽

Chen Su ◽

Le Dou ◽

...

Keyword(s):

Wastewater Treatment ◽

Nitrogen Removal ◽

Safety Assessment ◽

Heterotrophic Nitrification ◽

Aerobic Denitrification

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Effects of Hydraulic Retention Time and Influent Nitrate-N Concentration on Nitrogen Removal and the Microbial Community of an Aerobic Denitrification Reactor Treating Recirculating Marine Aquaculture System Effluent

Water ◽

10.3390/w12030650 ◽

2020 ◽

Vol 12 (3) ◽

pp. 650 ◽

Cited By ~ 1

Author(s):

Xiefa Song ◽

Xiaohan Yang ◽

Eric Hallerman ◽

Yuli Jiang ◽

Zhitao Huang

Keyword(s):

Microbial Community ◽

Nitrogen Removal ◽

Retention Time ◽

Hydraulic Retention Time ◽

Aerobic Denitrification ◽

Marine Aquaculture ◽

Nitrate N ◽

Aquaculture System ◽

N Concentration ◽

Denitrification Reactor

The effects of hydraulic retention time (HRT) and influent nitrate-N concentration on nitrogen removal and the microbial community composition of an aerobic denitrification reactor treating recirculating marine aquaculture system effluent were evaluated. Results showed that over 98% of nitrogen was removed and ammonia-N and nitrite-N levels were below 1 mg/L when influent nitrate-N was below 150 mg/L and HRT over 5 h. The maximum nitrogen removal efficiency and nitrogen removal rate were observed at HRT of 6 or 7 h when influent nitrate-N was 150 mg/L. High-throughput DNA sequencing analysis revealed that the microbial phyla Proteobacteria and Bacteroidetes were predominant in the reactor, with an average relative total abundance above 70%. The relative abundance of denitrifying bacteria of genera Halomonas and Denitratisoma within the reactor decreased with increasing influent nitrate-N concentrations. Our results show the presence of an aerobically denitrifying microbial consortium with both expected and unexpected members, many of them relatively new to science. Our findings provide insights into the biological workings and inform the design and operation of denitrifying reactors for marine aquaculture systems.

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Identification and Characterization of Janthinobacterium svalbardensis F19, a Novel Low-C/N-Tolerant Denitrifying Bacterium

Applied Sciences ◽

10.3390/app9091937 ◽

2019 ◽

Vol 9 (9) ◽

pp. 1937 ◽

Cited By ~ 5

Author(s):

Yinyan Chen ◽

Peng Jin ◽

Zhiwen Cui ◽

Tao Xu ◽

Ruojin Zhao ◽

...

Keyword(s):

Nitrogen Removal ◽

Removal Rate ◽

Pcr Amplification ◽

Domestic Wastewater ◽

Heterotrophic Nitrification ◽

Aerobic Denitrification ◽

Nitrite Accumulation ◽

Specific Pcr ◽

Domestic Wastewater Treatment ◽

Initial Ph

Herein, we isolated Janthinobacterium svalbardensis F19 from sludge sediment. Strain F19 can simultaneously execute heterotrophic nitrification and aerobic denitrification under aerobic conditions. The organism exhibited efficient nitrogen removal at a C/N ratio of 2:1, with an average removal rate of 0.88 mg/L/h, without nitrite accumulation. At a C/N ratio of 2, an initial pH of 10.0, a culturing temperature of 25 °C, and sodium acetate as the carbon source, the removal efficiencies of ammonium, nitrate, nitrite, and hydroxylamine were 96.44%, 92.32%, 97.46%, and 96.69%, respectively. The maximum removal rates for domestic wastewater treatment for ammonia and total nitrogen were 98.22% and 92.49%, respectively. Gene-specific PCR amplification further confirmed the presence of napA, hao, and nirS genes, which may contribute to the heterotrophic nitrification and aerobic denitrification capacity of strain F19. These results indicate that this bacterium has potential for efficient nitrogen removal at low C/N ratios from domestic wastewater.

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