Heterotrophic nitrification and aerobic denitrification by a novel Acinetobacter sp. TAC-1 at low temperature and high ammonia nitrogen

2021 ◽  
pp. 125620
Author(s):  
Tiantao Zhao ◽  
Peipei Chen ◽  
Lijie Zhang ◽  
Lei Zhang ◽  
Yanhui Gao ◽  
...  
Keyword(s):  
Low Temperature ◽  
Ammonia Nitrogen ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
High Ammonia ◽  
Acinetobacter Sp

Screening and Characterization of a Bacterium Capable of Simultaneous Heterotrophic Nitrification and Aerobic Denitrification at High Concentrations of Ammonia-Nitrogen

2014 ◽  
Vol 665 ◽  
pp. 487-490
Author(s):  
Te Wang ◽  
Zhao Xia Liu ◽  
Mei Juan Wu ◽  
Fu Hui Kang ◽  
Qing Chen ◽  
...  
Keyword(s):  
16S Rdna ◽  
Ammonia Nitrogen ◽  
Heterotrophic Nitrification ◽  
Denitrification Rate ◽  
Aerobic Denitrification ◽  
Optimal Conditions ◽  
Carbon And Nitrogen ◽  
Mass Ratios ◽  
High Concentrations ◽  
Denitrification Process

A bacterium capable of simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of ammonia-nitrogen was screened and identified and the denitrification property was investigated in this paper. The strain was isolated from aeration tank of wastewater disposed by activated sludge and analyzed and identified by 16S rDNA. The effects of different carbon sources and carbon and nitrogen mass ratios on denitrification rate were studied. The changes of various forms of ammonia-nitrogens during the simultaneous heterotrophic nitrification and aerobic denitrification process were characterized. A strain capable of simultaneous heterotrophic nitrification and aerobic denitrification at 600 mg/L nitrogen concentration has been isolated and screened. Comparison of its 16S rDNA sequence showed 100% similarity to Bacillus licheniformis strain Lr124/6. The strain was named as Bacillus sp. A22. The optimal conditions for degradation of ammonia-nitrogen by Bacillus sp. A22 were trisodium citrate as carbon source and carbon and nitrogen mass ratios of 10. The denitrification rate was 98.2% after 96 h of culture under the optimal conditions and there was hardly any intermediates accumulation in the denitrification process. It has practical applications that the denitrification can be performed efficiently at high concentrations of ammonia-nitrogen by method of simultaneous heterotrophic nitrification and aerobic denitrification by Bacillus sp. A22 in nitrogen purification treatment of wastewater with high concentrations of ammonia-nitrogen.

scholarly journals Heterotrophic nitrification and aerobic denitrification at low nutrient conditions by a newly isolated bacterium, Acinetobacter sp. SYF26

Microbiology ◽  
2015 ◽  
Vol 161 (4) ◽  
pp. 829-837 ◽  
Author(s):  
Jun-feng Su ◽  
Lin Guo ◽  
Shao-fei Yang ◽  
Kai Zhang ◽  
Ting-lin Huang ◽  
...  
Keyword(s):  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Acinetobacter Sp ◽  
Nutrient Conditions

Simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of NaCl and ammonia nitrogen by Halomonas bacteria

2017 ◽  
Vol 76 (2) ◽  
pp. 386-395 ◽  
Author(s):  
Te Wang ◽  
Jian Li ◽  
Ling Hua Zhang ◽  
Ying Yu ◽  
Yi Min Zhu
Keyword(s):  
Removal Rate ◽  
Sodium Succinate ◽  
Bacterial Genome ◽  
Ammonia Nitrogen ◽  
Compatible Solute ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Sequencing Analysis ◽  
N Removal ◽  
High Concentrations

To improve the efficiency of simultaneous heterotrophic nitrification and aerobic denitrification (SND) at high concentrations of NaCl and ammonia nitrogen (NH4+—N), we investigated the SND characteristics of Halomonas bacteria with the ability to synthesize the compatible solute ectoine. Halomonas sp. strain B01, which was isolated, screened and identified in this study, could simultaneously remove nitrogen (N) by SND and synthesize ectoine under high NaCl conditions. Gene cloning and sequencing analysis indicated that this bacterial genome contains ammonia monooxygenase (amoA) and nitrate reductase (narH) genes. Optimal conditions for N removal in a solution containing 600 mg/L NH4+–N were as follows: sodium succinate supplied as organic carbon (C) source at a C/N ratio of 5, pH 8 and shaking culture at 90 rpm. The N removal rate was 96.0% under these conditions. The SND by Halomonas sp. strain B01 was performed in N removal medium containing 60 g/L NaCl and 4,000 mg/L NH4+–N; after 180 h the residual total inorganic N concentration was 21.7 mg/L and the N removal rate was 99.2%. Halomonas sp. strain B01, with the ability to synthesize the compatible solute ectoine, could simultaneously tolerate high concentrations of NaCl and NH4+–N and efficiently perform N removal by SND.

scholarly journals Nitrogen Removal Characteristics of Pseudomonas putida Y-9 Capable of Heterotrophic Nitrification and Aerobic Denitrification at Low Temperature

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Yi Xu ◽  
Tengxia He ◽  
Zhenlun Li ◽  
Qing Ye ◽  
Yanli Chen ◽  
...  
Keyword(s):  
Low Temperature ◽  
Pseudomonas Putida ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Heterotrophic Nitrification ◽  
Oxidation Products ◽  
Aerobic Denitrification ◽  
Nitrification Rate ◽  
High Concentration ◽  
Nitrate And Nitrite

The cold-adapted bacterium Pseudomonas putida Y-9 was investigated and exhibited excellent capability for nitrogen removal at 15°C. The strain capable of heterotrophic nitrification and aerobic denitrification could efficiently remove ammonium, nitrate, and nitrite at an average removal rate of 2.85 mg, 1.60 mg, and 1.83 mg NL−1 h−1, respectively. Strain Y-9 performed nitrification in preference to denitrification when ammonium and nitrate or ammonium and nitrite coexisted in the solution. Meantime, the presence of nitrate had no effect on the ammonium removal rate of strain Y-9, and yet the presence of high concentration of nitrite would inhibit the cell growth and decrease the nitrification rate. The experimental results indicate that P. putida Y-9 has potential application for the treatment of wastewater containing high concentrations of ammonium along with its oxidation products at low temperature.

Sign in / Sign up

Export Citation Format

Share Document