Simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of NaCl and ammonia nitrogen by Halomonas bacteria

2017 ◽  
Vol 76 (2) ◽  
pp. 386-395 ◽  
Author(s):  
Te Wang ◽  
Jian Li ◽  
Ling Hua Zhang ◽  
Ying Yu ◽  
Yi Min Zhu
Keyword(s):  
Removal Rate ◽  
Sodium Succinate ◽  
Bacterial Genome ◽  
Ammonia Nitrogen ◽  
Compatible Solute ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Sequencing Analysis ◽  
N Removal ◽  
High Concentrations

To improve the efficiency of simultaneous heterotrophic nitrification and aerobic denitrification (SND) at high concentrations of NaCl and ammonia nitrogen (NH4+—N), we investigated the SND characteristics of Halomonas bacteria with the ability to synthesize the compatible solute ectoine. Halomonas sp. strain B01, which was isolated, screened and identified in this study, could simultaneously remove nitrogen (N) by SND and synthesize ectoine under high NaCl conditions. Gene cloning and sequencing analysis indicated that this bacterial genome contains ammonia monooxygenase (amoA) and nitrate reductase (narH) genes. Optimal conditions for N removal in a solution containing 600 mg/L NH4+–N were as follows: sodium succinate supplied as organic carbon (C) source at a C/N ratio of 5, pH 8 and shaking culture at 90 rpm. The N removal rate was 96.0% under these conditions. The SND by Halomonas sp. strain B01 was performed in N removal medium containing 60 g/L NaCl and 4,000 mg/L NH4+–N; after 180 h the residual total inorganic N concentration was 21.7 mg/L and the N removal rate was 99.2%. Halomonas sp. strain B01, with the ability to synthesize the compatible solute ectoine, could simultaneously tolerate high concentrations of NaCl and NH4+–N and efficiently perform N removal by SND.

Screening and Characterization of a Bacterium Capable of Simultaneous Heterotrophic Nitrification and Aerobic Denitrification at High Concentrations of Ammonia-Nitrogen

2014 ◽  
Vol 665 ◽  
pp. 487-490
Author(s):  
Te Wang ◽  
Zhao Xia Liu ◽  
Mei Juan Wu ◽  
Fu Hui Kang ◽  
Qing Chen ◽  
...  
Keyword(s):  
16S Rdna ◽  
Ammonia Nitrogen ◽  
Heterotrophic Nitrification ◽  
Denitrification Rate ◽  
Aerobic Denitrification ◽  
Optimal Conditions ◽  
Carbon And Nitrogen ◽  
Mass Ratios ◽  
High Concentrations ◽  
Denitrification Process

A bacterium capable of simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of ammonia-nitrogen was screened and identified and the denitrification property was investigated in this paper. The strain was isolated from aeration tank of wastewater disposed by activated sludge and analyzed and identified by 16S rDNA. The effects of different carbon sources and carbon and nitrogen mass ratios on denitrification rate were studied. The changes of various forms of ammonia-nitrogens during the simultaneous heterotrophic nitrification and aerobic denitrification process were characterized. A strain capable of simultaneous heterotrophic nitrification and aerobic denitrification at 600 mg/L nitrogen concentration has been isolated and screened. Comparison of its 16S rDNA sequence showed 100% similarity to Bacillus licheniformis strain Lr124/6. The strain was named as Bacillus sp. A22. The optimal conditions for degradation of ammonia-nitrogen by Bacillus sp. A22 were trisodium citrate as carbon source and carbon and nitrogen mass ratios of 10. The denitrification rate was 98.2% after 96 h of culture under the optimal conditions and there was hardly any intermediates accumulation in the denitrification process. It has practical applications that the denitrification can be performed efficiently at high concentrations of ammonia-nitrogen by method of simultaneous heterotrophic nitrification and aerobic denitrification by Bacillus sp. A22 in nitrogen purification treatment of wastewater with high concentrations of ammonia-nitrogen.

scholarly journals Biochar enhanced the nitrogen removal performance of heterotrophic nitrification-aerobic denitrification strain pseudomonas fluorescent sp. Z03 for insufficient carbon source wastewater under low temperature

2021 ◽  
Author(s):  
Jie Jiang ◽  
XiaoyanXu ◽  
Zhina Guo ◽  
Lianglun Sun ◽  
Meizhen Tang
Keyword(s):  
Electron Transfer ◽  
Low Temperature ◽  
Carbon Source ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Heterotrophic Nitrification ◽  
Redox Activity ◽  
Aerobic Denitrification ◽  
Control Group ◽  
N Removal

Abstract In this study, biochar BC400 and BC700 were prepared, characterized and coupled with heterotrophic nitrification-aerobic denitrification (HNAD) strain Z03 for nitrogen removal experiments. The characterization results showed that BC700 has a higher specific surface area and a more complex multilayered pore structure, with increased aromatic condensation and higher crystallinity. BC400 and BC700 both have good redox activity, while BC400 has stronger electron donor capacities and BC700 owns better electron transfer properties. In addition, both BC400 and BC700 contain relatively high levels of dissolved organic carbon (DOC), reaching at 62.95 and 51.617mg/g respectively. BC400/BC700 coupled with strain Z03 can significantly improve the NH4+-N removal performance of low-temperature and low C/N wastewater compared with the control group. At a dosage of 4.0 g/L, the removal rate of NH4+-N reached to 95.16% (BC400 + Z03) and 84.37% (BC700 + Z03) within 72h, respectively. Higher than the sum of adsorption by BC400/BC700 (16.19%/18.85%) and microbial degradation (41.03%). Besides, the BC400 + BC700 + Z03 NH4+-N removal systems provide higher nitrogen removal efficiencies than BC400/BC700 + Z03 nitrogen removal systems. When the dosage (BC400 + BC700, mass ratio 5:1) reaches 3.0g/L, it can achieve more than 90% NH4+-N removal rate within 48h. The reasons for the promotion of biochar on microbial denitrification were analyzed as follows: 1) DOC can provide an additional carbon source for microorganisms; 2) biochar, as a pH buffer, can neutralize the acidity due to nitrification; 3) BC400 and BC700, as materials with good redox activity, may play a role in promoting the activity of electron transfer system and enzyme activity.

scholarly journals Identification and Characterization of Janthinobacterium svalbardensis F19, a Novel Low-C/N-Tolerant Denitrifying Bacterium

2019 ◽  
Vol 9 (9) ◽  
pp. 1937 ◽  
Author(s):  
Yinyan Chen ◽  
Peng Jin ◽  
Zhiwen Cui ◽  
Tao Xu ◽  
Ruojin Zhao ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Pcr Amplification ◽  
Domestic Wastewater ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Nitrite Accumulation ◽  
Specific Pcr ◽  
Domestic Wastewater Treatment ◽  
Initial Ph

Herein, we isolated Janthinobacterium svalbardensis F19 from sludge sediment. Strain F19 can simultaneously execute heterotrophic nitrification and aerobic denitrification under aerobic conditions. The organism exhibited efficient nitrogen removal at a C/N ratio of 2:1, with an average removal rate of 0.88 mg/L/h, without nitrite accumulation. At a C/N ratio of 2, an initial pH of 10.0, a culturing temperature of 25 °C, and sodium acetate as the carbon source, the removal efficiencies of ammonium, nitrate, nitrite, and hydroxylamine were 96.44%, 92.32%, 97.46%, and 96.69%, respectively. The maximum removal rates for domestic wastewater treatment for ammonia and total nitrogen were 98.22% and 92.49%, respectively. Gene-specific PCR amplification further confirmed the presence of napA, hao, and nirS genes, which may contribute to the heterotrophic nitrification and aerobic denitrification capacity of strain F19. These results indicate that this bacterium has potential for efficient nitrogen removal at low C/N ratios from domestic wastewater.

Tertiary treatment of municipal wastewater using bioflocculating micro-algae

1991 ◽  
Vol 18 (6) ◽  
pp. 940-944 ◽  
Author(s):  
J. B. Sérodes ◽  
E. Walsh ◽  
O. Goulet ◽  
J. de la Noue ◽  
C. Lescelleur
Keyword(s):  
Removal Efficiency ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Batch Reactor ◽  
Ammonia Nitrogen ◽  
Design Criteria ◽  
Tertiary Treatment ◽  
Deep Basin ◽  
Sequential Batch Reactor ◽  
N Removal

Design criteria of a pilot plant for treating secondary municipal effluents using filamentous, bioflocculating micro-algae were evaluated. Using a sequential batch reactor, the best removal rate of ammonia nitrogen was reached for 25% draw volume; at 20–22 °C, up to four cycles per day could be achieved giving a removal efficiency of approximately 2 g of N per day and per square meter of basin (200 mm deep) with negligible nitrogen residual; increasing the water level by increments of 200 mm (from 200 to 600 mm) increased the N removal efficiency in a way similar to an increase in the number of renewals per day on a 200 mm deep basin. The dominant micro-algae (Chlorhormidium) was heavily influenced by the water temperature. Key words: micro-algae, municipal wastewater, water treatment, ammonia nitrogen, removal rate, removal efficiency.

Ammonia nitrogen removal from acetylene purification wastewater from a PVC plant by struvite precipitation

2016 ◽  
Vol 74 (2) ◽  
pp. 508-515 ◽  
Author(s):  
Lei Zhu ◽  
DeMing Dong ◽  
XiuYi Hua ◽  
ZhiYong Guo ◽  
DaPeng Liang
Keyword(s):  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Precipitation Method ◽  
Minor Effect ◽  
N Removal ◽  
Electron Microscopy Analysis ◽  
Typical Morphology ◽  
Coarse Surface ◽  
Struvite Precipitation ◽  
High Concentrations

Acetylene purification wastewater (APW) usually contains high concentrations of ammonia nitrogen (NH4-N), which is generated during the production of acetylene in a polyvinylchloride manufacturing plant. In this study, a struvite precipitation method was selected to remove NH4-N from the APW. Laboratory-scale batch experiments were performed to investigate the effects of the initial APW pH, phosphate (PO43−) concentration, magnesium (Mg2+) concentration, and sources of PO43− and Mg2+ on NH4-N removal. The results indicated that the initial APW pH had a significant effect on the removal of NH4-N, while the other factors had relatively minor effect. The NH4-N could be effectively removed at an optimum initial APW pH of 9.5, when Na2HPO4·12H2O and MgSO4·7H2O were both applied to NH4-N at a ratio of 1.2. Under these conditions, the efficiency of removal of NH4-N, total nitrogen and chemical oxygen demand were 85%, 84% and 18%, respectively. The X-ray diffraction analysis indicated that the precipitates were dominated by struvite. The scanning electron microscopy analysis of the precipitates showed a typical morphology of stick-like and prismatic crystals with coarse surface. The energy dispersive spectroscopy analysis indicated that the precipitates contained P, O, Mg and Ca.

Effect on Bed Material Heights to the Performance of ZCBAF in the Treatment of Micro-Polluted Raw Water

2012 ◽  
Vol 209-211 ◽  
pp. 2053-2057
Author(s):  
Jin Xiang Liu ◽  
Shui Bo Xie ◽  
Chun Ning Cheng ◽  
Jin Sheng Lou ◽  
Shi You Li
Keyword(s):  
Removal Efficiency ◽  
Removal Rate ◽  
Ammonia Nitrogen ◽  
Test Results ◽  
Raw Water ◽  
Hydraulic Loading ◽  
N Removal ◽  
The Media ◽  
Aerated Filter ◽  
Bed Material

The effect of bed material heights on treatment performance of pollutants from micro-polluted raw Water was studied in zeolite - Ceramics biological aerated filter(ZCBAF) technology. The test results showed the removal rate of CODMn, NH4+-N and UV254 will improve with the increase of media height, most of CODMn and UV254 were removed within the first 440mm , when the media height over 440mm, the effect of increase height is inconspicuous removal, and ammonia-nitrogen removal has evident improvement during 220-440mm. At the media height of 20mm, 40mm and 60mm in ZCBAF respectively, the removal efficiency of CODMn is 18.05%，31.6% and 38.62% respectively, NH4+-N removal efficiency is 29.78%,81.28% and 93.02% respectively , and UV254 removal efficiency is 7.81%，10.11% and 11.26% respectively under the air/water ratio of 1:1 and the hydraulic loading of 1.2m3/(m2.h). Removal rate of CODMn was decreased with the increase of hydraulic loading, and removal effect of NH4+-N and UV254 had not big influence.

scholarly journals Experimental Study on a Closed-Cycle Humidification and Dehumidification System for Treating Wastewater Containing High Concentrations of Inorganic Salts and Organic Matter

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 671
Author(s):  
Jun Liu ◽  
Yong Sun ◽  
Sanjiang Yv ◽  
Jiaquan Wang ◽  
Kaixuan Hu
Keyword(s):  
Organic Matter ◽  
Industrial Wastewater ◽  
Removal Rate ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Filter Press ◽  
Inorganic Salts ◽  
Closed Cycle ◽  
Treatment Performance ◽  
High Concentrations

Industrial wastewater contains high concentrations of inorganic salts and organic matter. This experiment studied a system for treating wastewater containing high concentrations of inorganic salts and organic matter. The setup consists of a closed-cycle humidification and dehumidification system and a filter press. Chemical wastewater was used as the treatment solution, and the treatment performance of the system was tested and analyzed. The system effectively reduced the chemical oxygen demand (COD), electric conductivity (EC), total nitrogen (TN), and ammonia nitrogen (NH4-N) in the wastewater and, at the same time, dehydrated sludge was obtained through a filter press. The system maintains a stable removal rate of each index (COD, EC, TN, and NH4-N) in wastewater and can remove inorganic salts and organic matter from wastewater. The system can successfully treat industrial wastewater containing high concentrations of inorganic salts and organic matter.

Isolation and Characteristics of Heterotrophic Nitrification-Aerobic Denitrification Bacterium, Bacillus cereus X7 at High Salinity

2013 ◽  
Vol 864-867 ◽  
pp. 111-114 ◽  
Author(s):  
Ru Lei Yao ◽  
Li Na Qiu ◽  
Wei Wei Zhang ◽  
Ai Jun Gong ◽  
Zi Yu Wang ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Nitrogen Removal ◽  
High Salinity ◽  
Removal Rate ◽  
Aerobic Denitrification ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
N Removal ◽  
Nacl Concentration ◽  
Biological Nitrogen

Biological nitrogen removal has been focused on in wastewater treatment field recently. A strain X7 was isolated from the sediment of pickle foodstuff wastewater. Based on its 16S rDNA sequence analysis, X7 was identified as Bacillus cereus. At NaCl concentration of 20 g/L, NH4+-N removal rate achieved 99.18%, when NO2--N and NO3--N removal rates were 77.24% and 68.6%, respectively. When NaCl concentration ranged from 0 to 40 g/L, the removal rate of NH4+-N was more than 97.59%. Therefore, due to the high nitrogen removal rate and excellent salt tolerance, Bacillus cereus X7 had a broad application prospect in the biodenitrification of brine wastewater.

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