A triple-amplification differential pulse voltammetry for sensitive detection of DNA based on exonuclease III, strand displacement reaction and terminal deoxynucleotidyl transferase

2019 ◽  
Vol 143 ◽  
pp. 111609 ◽  
Author(s):  
Xinyi Huang ◽  
Wuceng Niu ◽  
Jiayi Wu ◽  
Yang Wang ◽  
Chaorui Li ◽  
...  
Keyword(s):  
Differential Pulse Voltammetry ◽  
Differential Pulse ◽  
Sensitive Detection ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Exonuclease Iii ◽  
Pulse Voltammetry ◽  
Strand Displacement Reaction

An enzyme free electrochemical biosensor for sensitive detection of miRNA with a high discrimination factor by coupling the strand displacement reaction and catalytic hairpin assembly recycling

The Analyst ◽  
2017 ◽  
Vol 142 (21) ◽  
pp. 4116-4123 ◽  
Author(s):  
Juan Yao ◽  
Zhang Zhang ◽  
Zhenghua Deng ◽  
Youqiang Wang ◽  
Yongcan Guo
Keyword(s):  
Electrochemical Biosensor ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Discrimination Factor ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
High Discrimination ◽  
Catalytic Hairpin Assembly ◽  
Strand Displacement Reaction

Sensitive and selective detection of miRNA based on the toehold-mediated strand displacement reaction (SDR) and non-enzymatic catalytic hairpin reaction (CHA) recycling.

A dual-cycling biosensor for target DNA detection based on the toehold-mediated strand displacement reaction and exonuclease III assisted amplification

2018 ◽  
Vol 42 (6) ◽  
pp. 4714-4718 ◽  
Author(s):  
Yu Ling ◽  
Xiao Fang Zhang ◽  
Xiao Hui Chen ◽  
Li Liu ◽  
Xiao Hu Wang ◽  
...  
Keyword(s):  
Dna Detection ◽  
Dna Biosensor ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Exonuclease Iii ◽  
Target Dna ◽  
Strand Displacement Reaction

Based on the toehold-mediated strand displacement reaction and exonuclease III assisted amplification, a sensitive and simple target DNA biosensor was established.

scholarly journals Application of Experimental Design by Differential Pulse Voltammetry for Determination of Rare Elements as Complexes with Diethylenetriaminepentaacetic Acid (DTPA)

2019 ◽  
Vol 8 (2S7) ◽  
pp. 33-37
Keyword(s):  
Experimental Design ◽  
Differential Pulse Voltammetry ◽  
Differential Pulse ◽  
Modern Technology ◽  
Sensitive Detection ◽  
Limit Values ◽  
Rare Elements ◽  
Icp Ms ◽  
Burman Design ◽  
Pulse Voltammetry

Rare earth elements have been known as the importance material in modern technology. Since the presence of these materials in environment is very small concentration, the development method for fast and sensitive detection is challenging. Instead of relatively expensive ICP-MS instrument, the utilization of voltammetry method to answer the task of fast and sensitive detection is considerably cheaper. In the present work, a complex of Gadolinium (Gd) and Terbium (Tb) with diethylentriaminepentaacetic acid (DTPA) ligand was studied by experimental design to obtain the optimum condition detection of differential pulse voltammetry. The Plackett-Burman design was selected as the method. The positive response was optimized based on Box-Behnken. Optimum experimental condition was achieved by using scan rate of 0.05 Vs-1 , amplitude pulse of 0.5489 V, ligand concentration of 267.67 mgL-1 , pH of 5.15 and the electrolyte solution of ammonium chloride of 0.1 M. Under the optimum experimental condition, the precision and accuracy were found as 99.7% and 97.4% for Gd-DTPA; and 99.5% and 91.4% for Tb-DTPA. The detection limit and quantization limit values for Gd-DTPA were 27.11 mgL-1 and 92.33 mgL-1 , respectively; and Tb-DTPA were 27.69 mgL-1 and 92.34 mgL-1 , respectively.

A homogeneous fluorescent biosensing strategy for highly sensitive detection of DNA based on a programmed entropy-driven strand displacement reaction and DNAzyme for dual recycling amplification

2019 ◽  
Vol 11 (12) ◽  
pp. 1613-1619 ◽  
Author(s):  
Yujian Li ◽  
Xiaojuan Ding ◽  
Dandan Li ◽  
Haiping Wu ◽  
Wei Huang ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Fluorescent Biosensing ◽  
Recycling Amplification ◽  
Highly Sensitive Detection ◽  
Strand Displacement Reaction

In this study, a homogeneous fluorescent biosensing strategy is described for the detection of DNA based on an entropy-driven strand displacement reaction and DNAzyme.

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