Metabolic engineering in the biotechnological production of organic acids in the tricarboxylic acid cycle of microorganisms: Advances and prospects

2015 ◽  
Vol 33 (6) ◽  
pp. 830-841 ◽  
Author(s):  
Xian Yin ◽  
Jianghua Li ◽  
Hyun-dong Shin ◽  
Guocheng Du ◽  
Long Liu ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Organic Acids ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Biotechnological Production ◽  
Acid Cycle

scholarly journals Metabolic Engineering of the Tricarboxylic Acid Cycle for Improved Lysine Production by Corynebacterium glutamicum

2009 ◽  
Vol 75 (24) ◽  
pp. 7866-7869 ◽  
Author(s):  
Judith Becker ◽  
Corinna Klopprogge ◽  
Hartwig Schröder ◽  
Christoph Wittmann
Keyword(s):  
Metabolic Engineering ◽  
Corynebacterium Glutamicum ◽  
Tricarboxylic Acid Cycle ◽  
Tca Cycle ◽  
Tricarboxylic Acid ◽  
Start Codon ◽  
Flux Analysis ◽  
Lysine Production ◽  
Acid Cycle ◽  
The Tca Cycle

ABSTRACT In the present work, lysine production by Corynebacterium glutamicum was improved by metabolic engineering of the tricarboxylic acid (TCA) cycle. The 70% decreased activity of isocitrate dehydrogenase, achieved by start codon exchange, resulted in a >40% improved lysine production. By flux analysis, this could be correlated to a flux shift from the TCA cycle toward anaplerotic carboxylation.

scholarly journals New conditions of HPLC analysis for separation and quantification of simple organic acids of tricarboxylic acid cycle in psoriasis

2020 ◽  
Author(s):  
Jiaxing Fan ◽  
Yiyang Liu ◽  
Shenmin Su ◽  
Yiqing Cao ◽  
Yunqiu Yu
Keyword(s):  
Organic Acids ◽  
High Performance ◽  
Aldol Condensation ◽  
Tricarboxylic Acid Cycle ◽  
Simultaneous Detection ◽  
Tricarboxylic Acid ◽  
Acid Cycle ◽  
Physiological Processes ◽  
Aconitic Acid ◽  
Important Pathway

AbstractTricarboxylic acid cycle is an important pathway and the metabolites of tricarboxylic acid cycle, simple organic acids, play important roles in many physiological processes. The new conditions of high performance liquid chromatography (HPLC) with improved separation selectivity were developed and optimized by choosing suitable store solvent, methanol, which can catalyze the reaction, aldol condensation, and facilitate the formation of new compound to improve simultaneous detection of nine metabolites of tricarboxylic acid cycle, including lactic acid, pyruvic acid, citric acid, alpha-ketoglutaric acid, malic acid, succinic acid, cis-aconitic acid, itaconic acid, and fumaric acid, and be applied in analysis of the nine metabolites in psoriasis mice skin. This study shows that in process of developing methods, expect conventional chromatographic condition, solvent should also be considered carefully.

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