Growth and synthesis of rubratoxin by Penicillium rubrum in a chemically defined medium fortified with organic acids and intermediates of the tricarboxylic acid cycle

1976 ◽  
Vol 59 (3) ◽  
pp. 137-142 ◽  
Author(s):  
C. O. Emeh ◽  
E. H. Marth
Keyword(s):  
Organic Acids ◽  
Tricarboxylic Acid Cycle ◽  
Defined Medium ◽  
Tricarboxylic Acid ◽  
Chemically Defined Medium ◽  
Acid Cycle ◽  
Penicillium Rubrum

UTILIZATION OF CARBOHYDRATES AND AMINO ACIDS BY MICROCOCCUS RADIODURANS

1960 ◽  
Vol 6 (3) ◽  
pp. 289-298 ◽  
Author(s):  
Harkison D. Raj ◽  
Frances L. Duryee ◽  
Anne M. Deeney ◽  
Chih H. Wang ◽  
Arthur W. Anderson ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Tricarboxylic Acid Cycle ◽  
Radioactive Tracer ◽  
Defined Medium ◽  
Tricarboxylic Acid ◽  
Chemically Defined Medium ◽  
Acid Cycle ◽  
Tracer Techniques ◽  
Simple Carbohydrates

The nutrition and metabolism of a recently isolated Micrococcus species resistant to closes of gamma radiation as high as 6 × 106 r.e.p. were studied by manometric and radioactive tracer techniques. Methionine, the only amino acid shown to be essential in a chemically defined medium, appears to be rapidly incorporated into the cells. DL-Glutamic acid is readily metabolized, the D isomer apparently after an initial lag. Among the simple carbohydrates, fructose, glucose, and glycerol are readily utilized. The operation of the tricarboxylic acid cycle is suggested by the oxidation of certain TCA intermediates. The prompt conversion of C-1 of gluconate to CO2, in the presence of glucose, may indicate that a C1–C5 cleavage pathway is operative for catabolism of glucose in this organism.

scholarly journals Analysis of organic acids of tricarboxylic acid cycle in plants using GC-MS, and system modeling

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Vinod Kumar ◽  
Anket Sharma ◽  
Renu Bhardwaj ◽  
Ashwani Kumar Thukral
Keyword(s):  
Organic Acids ◽  
Tricarboxylic Acid Cycle ◽  
System Modeling ◽  
Tricarboxylic Acid ◽  
Acid Cycle

scholarly journals Control of the production of exo-β-N-acetylglucosaminidase by Bacillus subtilis B. Derepression during gluconeogenesis and initial stages of sporulation

1973 ◽  
Vol 134 (1) ◽  
pp. 271-281 ◽  
Author(s):  
Stephen J. Brewer ◽  
Roger C. W. Berkeley
Keyword(s):  
Bacillus Subtilis ◽  
Exponential Growth ◽  
Phosphoenolpyruvate Carboxylase ◽  
Metal Ion ◽  
Tricarboxylic Acid Cycle ◽  
Carbon Sources ◽  
Defined Medium ◽  
Tricarboxylic Acid ◽  
Carboxylase Activity ◽  
Acid Cycle

1. The control of exo-β-N-acetylglucosaminidase (EC 3.2.1.30) production by Bacillus subtilis B growing on a chemically defined medium was studied. 2. The enzyme was repressed during exponential growth by those carbon sources that enter the glycolytic pathway above the level of phosphoenolpyruvate. When exponential growth ceased as a result of low concentrations of the nitrogen, carbon or metal ion components of the medium, the enzyme was formed and its amount could be increased by the addition of cell-wall fragments as inducer. 3. The enzyme was de-repressed and could be induced during exponential growth on non-glycolytic compounds metabolized directly into pyruvate, acetyl-CoA or tricarboxylic acid cycle intermediates. 4. The major difference in the metabolism of the organism utilizing these two groups of compound was the existence of high activities of phosphoenolpyruvate carboxylase required for gluconeogenesis. 5. It is concluded that the de-repression of glucosaminidase occurs when the only principal change detected in the intermediary metabolism of the organism was the presence of high activities of phosphoenolpyruvate carboxylase. 6. When the organism was grown on media containing repressing compounds, the enzyme was only de-repressed on entry of the cells into the initial stages of sporulation, where phosphoenolpyruvate carboxylase activity, even in the presence of excess of glucose, increased in parallel with glucosaminidase, neutral proteinase and alkaline phosphatase activities. 7. These results suggest a strong link, at the level of the tricarboxylic acid cycle, between the control of phosphoenolpyruvate carboxylase and the control of the de-repression of glucosaminidase and sporulation.

Metabolic engineering in the biotechnological production of organic acids in the tricarboxylic acid cycle of microorganisms: Advances and prospects

2015 ◽  
Vol 33 (6) ◽  
pp. 830-841 ◽  
Author(s):  
Xian Yin ◽  
Jianghua Li ◽  
Hyun-dong Shin ◽  
Guocheng Du ◽  
Long Liu ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Organic Acids ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Biotechnological Production ◽  
Acid Cycle

scholarly journals New conditions of HPLC analysis for separation and quantification of simple organic acids of tricarboxylic acid cycle in psoriasis

2020 ◽  
Author(s):  
Jiaxing Fan ◽  
Yiyang Liu ◽  
Shenmin Su ◽  
Yiqing Cao ◽  
Yunqiu Yu
Keyword(s):  
Organic Acids ◽  
High Performance ◽  
Aldol Condensation ◽  
Tricarboxylic Acid Cycle ◽  
Simultaneous Detection ◽  
Tricarboxylic Acid ◽  
Acid Cycle ◽  
Physiological Processes ◽  
Aconitic Acid ◽  
Important Pathway

AbstractTricarboxylic acid cycle is an important pathway and the metabolites of tricarboxylic acid cycle, simple organic acids, play important roles in many physiological processes. The new conditions of high performance liquid chromatography (HPLC) with improved separation selectivity were developed and optimized by choosing suitable store solvent, methanol, which can catalyze the reaction, aldol condensation, and facilitate the formation of new compound to improve simultaneous detection of nine metabolites of tricarboxylic acid cycle, including lactic acid, pyruvic acid, citric acid, alpha-ketoglutaric acid, malic acid, succinic acid, cis-aconitic acid, itaconic acid, and fumaric acid, and be applied in analysis of the nine metabolites in psoriasis mice skin. This study shows that in process of developing methods, expect conventional chromatographic condition, solvent should also be considered carefully.

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