Alkamides from the fruits of Piper longum and Piper nigrum displaying potent cell adhesion inhibition

The aim of the present study was to convert an Ayurvedic formulation, “trikatu churna”, into a polyherbal dispersible tablet. Standardization parameters were performed for raw materials, trikatu churna and its extract. The standardized extract was further used for formulation of the dispersible tablet. The evaluation of raw materials, trikatu churna, trikatu extract and dispersible tablet was done using preliminary phytochemical and physico-chemical tests. The pre- and post-compression parameters for polyherbal dispersible tablet formulation were within the acceptance range. The piperine content in Piper nigrum, Piper longum, trikatu extract and formulation was found to be 2.884mg/mL, 0.515mg/gm, 0.303mg/gm and 0.357mg/gm, respectively. The developed RP-HPLC method was found to be precise, accurate and reproducible for the estimation of piperine in raw materials and dispersible tablet. It can be concluded that the prepared dispersible tablet has overcome the disadvantages of churna, such as poor patient compliance and pungent taste, without altering its dose and chemical properties.

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Piperine- Review of Advances in Pharmacology

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2009.2.3.4 ◽

2009 ◽

Vol 2 (3) ◽

pp. 615-620 ◽

Cited By ~ 2

Author(s):

Amritpal Singh ◽

Sanjiv Duggal

Keyword(s):

Amino Acids ◽

Complementary Therapies ◽

Beta Carotene ◽

Piper Nigrum ◽

Piper Longum ◽

Extensive Data ◽

Recent Advances ◽

Wide Range ◽

Pharmacological Studies

Piperine, an alkaloid, is found in Piper longum L. and Piper nigrum L. The plants containing piperine are widely used in Alternative and Complementary Therapies for curing an array of disorders. Piperine obtained from botanical sources is about 98% pure. It is produced in the laboratory for chemical and medical purposes. Piperine is helpful in reducing inflammation, improving digestion, and relieving pain and asthma. It is reported to improve the production of serotonin, it may relieve stomach ulcerations. It improves the bioavailability of other nutritive substances including beta carotene, curcumin, selenium, pyroxidine, glucose, and amino acids. Extensive data has been complied in favour of piperine in a wide range of pharmacological studies. The review analyses recent advances in pharmacology research on piperine.

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Comparison of Disintegrins with Limited Variation in the RGD Loop in Their Binding to Purified Integrins αIIbβ3, αVβ3 and α5β1 and in Cell Adhesion Inhibition

Cell Adhesion and Communication ◽

10.3109/15419069409014213 ◽

1994 ◽

Vol 2 (6) ◽

pp. 491-501 ◽

Cited By ~ 77

Author(s):

Martin Pfaff ◽

Mary Ann McLane ◽

Lucia Beviglia ◽

Stefan Niewiarowski ◽

Rupert Timpl

Keyword(s):

Cell Adhesion ◽

Limited Variation ◽

Adhesion Inhibition

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Structure–function studies of peptides for cell adhesion inhibition: Identification of key residues by alanine mutation and peptide-truncation approach

Peptides ◽

10.1016/j.peptides.2007.07.003 ◽

2007 ◽

Vol 28 (8) ◽

pp. 1498-1508 ◽

Cited By ~ 4

Author(s):

Cheng Li ◽

Seetharama D. Satyanarayanajois

Keyword(s):

Cell Adhesion ◽

Structure Function ◽

Peptide Truncation ◽

Adhesion Inhibition ◽

Key Residues

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Cell Adhesion Inhibition by Glycoliposomes: Effects of Vesicle Diameter and Ligand Density

Tumor Biology ◽

10.1159/000030124 ◽

2000 ◽

Vol 21 (3) ◽

pp. 176-186 ◽

Cited By ~ 12

Author(s):

Renate Stahn ◽

Reinhard Zeisig

Keyword(s):

Cell Adhesion ◽

Ligand Density ◽

Adhesion Inhibition

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The plant alkaloid piperine as a potential inhibitor of ethidium bromide efflux in Mycobacterium smegmatis

Journal of Medical Microbiology ◽

10.1099/jmm.0.025734-0 ◽

2011 ◽

Vol 60 (2) ◽

pp. 223-229 ◽

Cited By ~ 27

Author(s):

Jing Jin ◽

Jiyu Zhang ◽

Na Guo ◽

Haihua Feng ◽

Lei Li ◽

...

Keyword(s):

Ethidium Bromide ◽

Mycobacterium Smegmatis ◽

Efflux Pump ◽

Black Pepper ◽

Piper Nigrum ◽

Fluorometric Method ◽

Piper Longum ◽

Carbonyl Cyanide ◽

Inhibition Ability ◽

Positive Controls

Piperine, a major plant alkaloid found in black pepper (Piper nigrum) and long pepper (Piper longum), has shown potential for inhibiting the efflux pump (EP) of Staphylococcus aureus. In this study, a modulation assay showed that piperine could decrease the MIC of ethidium bromide (EtBr) twofold at 32 μg ml−1 and fourfold at 64 μg ml−1 against Mycobacterium smegmatis mc2 155 ATCC 700084. A real-time, 96-well plate fluorometric method was employed to evaluate the EP inhibition ability of piperine in M. smegmatis. Reserpine, chlorpromazine, verapamil and carbonyl cyanide m-chlorophenylhydrazone were used as positive controls. Piperine significantly enhanced accumulation and decreased the efflux of EtBr in M. smegmatis, which suggests that it has the ability to inhibit mycobacterial EPs.

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