scholarly journals The Anti-Proliferative Effect of Cation Channel Blockers on T Lymphocytes Stimulated by Anti-CD3 and Anti-CD28

2015 ◽  
Vol 108 (2) ◽  
pp. 586a-587a
Author(s):  
Zoltan Varga ◽  
Zoltan Petho ◽  
Andras Balajthy ◽  
Adam Bartok ◽  
Sandor Somodi ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Cation Channel ◽  
Channel Blockers ◽  
Proliferative Effect

scholarly journals The anti-proliferative effect of cation channel blockers in T lymphocytes depends on the strength of mitogenic stimulation

2016 ◽  
Vol 171 ◽  
pp. 60-69 ◽  
Author(s):  
Zoltan Petho ◽  
Andras Balajthy ◽  
Adam Bartok ◽  
Krisztian Bene ◽  
Sandor Somodi ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Cation Channel ◽  
Channel Blockers ◽  
Mitogenic Stimulation ◽  
Proliferative Effect

scholarly journals Proliferative effect of the calcium channel blocker Nifedipine on human embryonic kidney cells

2018 ◽  
Vol 7 (7) ◽  
pp. 1226 ◽  
Author(s):  
Jamil L. Ahmad ◽  
Chukwudi C. Okebaram ◽  
Emmanuel O. Ogbuagu ◽  
Chidozie N. Ogbonnaya ◽  
Geoffrey C. Asobie
Keyword(s):  
Calcium Channel ◽  
Hek293 Cells ◽  
Cell Counting ◽  
Blue Dye ◽  
Channel Blockers ◽  
Human Embryonic Kidney ◽  
Proliferative Effect ◽  
Increased Risk ◽  
Chronic Use

Background: Numerous epidemiological studies have shown a positive as well as negative association between chronic use of calcium channel blockers and the increased risk of developing cancer. However, these associations were enmeshed with controversies in the absence of laboratory based studies to back up those claims. The aim was to determine in mechanistic terms the association between the long-term administrations of nifedipineand increased risk of developing cancer with the aid of human embryonic kidney (HEK293) cell line.Methods: Cell counting using the Trypan blue dye exclusion and 3-[4, 5-Dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT) assays were used to investigate the effect of nifedipine on the growth pattern of HEK293 cells.Results: Nifedipine had a proliferative effect on HEK293 cells growth and this proliferation is more profound at low concentrations of nifedipine than high concentrations and the proliferation was statistically significant (p<0.01).Conclusions: The chronic use of nifedipine is associated with increased proliferation of cells with concomitant elevation of polyamines concentration and elevated polyamine levels have been implicated in many malignant transformations and hence, these provide possible explanation on the link between long term use of nifedipine and development of some human cancers.

Isolation and Total Synthesis of Icumazoles and Noricumazoles-Antifungal Antibiotics and Cation-Channel Blockers from Sorangium cellulosum

2011 ◽  
Vol 51 (5) ◽  
pp. 1256-1260 ◽  
Author(s):  
Jenny Barbier ◽  
Rolf Jansen ◽  
Herbert Irschik ◽  
Stefan Benson ◽  
Klaus Gerth ◽  
...  
Keyword(s):  
Total Synthesis ◽  
Cation Channel ◽  
Channel Blockers ◽  
Sorangium Cellulosum ◽  
Antifungal Antibiotics

scholarly journals Lamprey buccal gland secretory protein-2 (BGSP-2) inhibits human T lymphocyte proliferation

2010 ◽  
Vol 56 (2) ◽  
pp. 252-258 ◽  
Author(s):  
Jing Sun ◽  
Shuiyan Yu ◽  
Zhuang Xue ◽  
Cenjie Liu ◽  
Yu Wu ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Lymphocyte Proliferation ◽  
Secretory Protein ◽  
Minimum Concentration ◽  
Human T Lymphocytes ◽  
Proliferative Effect ◽  
T Lymphocyte Proliferation ◽  
Human T Lymphocyte ◽  
Host Fishes

Abstract Lamprey is a representative of the agnathans, the most ancient class of vertebrates. Parasitic lampreys secrete anticoagulant from their buccal glands and prevent blood coagulation of host fishes. We identified a buccal gland secretory protein-2 (BGSP-2) from a buccal gland cDNA library of Lampetra japonica. The full-length BGSP-2 gene was cloned and the recombinant BGSP-2 protein was generated. The role of BGSP-2 on lymphocyte proliferation was studied by examining its effects on human T lymphocytes. We found that lamprey BGSP-2 was able to effectively block the proliferation of T cells in vitro by inducing G1/S cell cycle arrest. Furthermore, it inhibited the proliferation of human T lymphocytes stimulated by phytohemagglutinin (PHA) at a minimum concentration of 0.μg/ml. Our data suggest that lamprey BGSP-2 is able to block the mitosis of human T lymphocytes at the G1/S point, and has the potential of anti-proliferative effect on PHA-activated T lymphocytes.

Role of phosphoinositide 3-kinase in the nonselective cation channel activation by endothelin-1/endothelinBreceptor

2003 ◽  
Vol 284 (2) ◽  
pp. C506-C510 ◽  
Author(s):  
Yoshifumi Kawanabe ◽  
Nobuo Hashimoto ◽  
Tomoh Masaki
Keyword(s):  
Chinese Hamster ◽  
Pi3k Pathway ◽  
Cation Channel ◽  
Channel Blockers ◽  
Nonselective Cation Channel ◽  
Channel Activation ◽  
Endothelin 1 ◽  
Ovarian Cells ◽  
Phosphoinositide 3 Kinase

We recently demonstrated that endothelin-1 (ET-1) activates two types of Ca2+-permeable nonselective cation channel (designated NSCC-1 and NSCC-2) in Chinese hamster ovarian cells expressing endothelinB receptor (CHO-ETBR). These channels can be discriminated using the Ca2+ channel blockers, LOE 908 and SK&F 96365. LOE 908 is a blocker of NSCC-1 and NSCC-2, whereas SK&F 96365 is a blocker of NSCC-2. In this study, we investigated the possible role of phosphoinositide 3-kinase (PI3K) in the ET-1-induced activation of NSCCs in CHO-ETBR using wortmannin and LY-294002, inhibitors of PI3K. ET-1-induced Ca2+ influx was partially inhibited in CHO-ETBR pretreated with wortmannin or LY-294002. In contrast, addition of wortmannin or LY-294002 after stimulation with ET-1 did not suppress Ca2+ influx. The Ca2+ channels activated by ET-1 in wortmannin- or LY-294002-treated CHO-ETBR were sensitive to LOE 908 and resistant to SK&F 96365. In conclusion, NSCC-2 is stimulated by ET-1 via PI3K-dependent cascade, whereas NSCC-1 is stimulated independently of the PI3K pathway. Moreover, PI3K seems to be required for the initiation of the Ca2+ entry through NSCC-2 but not for its maintenance.

TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach

2003 ◽  
Vol 284 (4) ◽  
pp. G604-G616 ◽  
Author(s):  
Young Mee Lee ◽  
Byung Joo Kim ◽  
Hyun Jin Kim ◽  
Dong Ki Yang ◽  
Mei Hong Zhu ◽  
...  
Keyword(s):  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Cation Channel ◽  
Trp Channel ◽  
Channel Blockers ◽  
Nonselective Cation Channel ◽  
Rt Pcr ◽  
Pipette Solution ◽  
Gastric Myocytes ◽  
Transient Receptor

We investigated which transient receptor potential (TRP) channel is responsible for the nonselective cation channel (NSCC) activated by carbachol (CCh) in murine stomach with RT-PCR and the electrophysiological method. All seven types of TRP mRNA were detected in murine stomach with RT-PCR. When each TRP channel was expressed, the current-voltage relationship of mTRP5 was most similar to that recorded in murine gastric myocytes. mTRP5 showed a conductance order of Cs+ > K+ > Na+, similar to that in the murine stomach. With 0.2 mM GTPγS in the pipette solution, the current was activated transiently in both NSCC in the murine stomach and the expressed mTRP5. Both NSCC activated by CCh in murine stomach and mTRP5 were inhibited by intracellularly applied anti-Gq/11 antibody, PLC inhibitor U-73122, IICR inhibitor 2-aminoethoxydiphenylborate, and nonspecific cation channel blockers La3+ and flufenamate. There were two other unique properties. Both the native NSCC and mTRP5 were activated by 1-oleoyl-2-acetyl-sn-glycerol. Without the activation of NSCC by CCh, the NSCC in murine stomach was constitutively active like mTRP5. From the above results, we suggest that mTRP5 might be a candidate for the NSCC activated by ACh or CCh in murine stomach.

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