Label-free Multiscale Transport Imaging of the Living Cell

A label-free activatable aptamer probe was developed for cancer cell detection through recognition-switched split DNAzyme activity on a living cell surface.

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Development of SPR Imaging-Impedance Sensor for Multi-Parametric Living Cell Analysis

Sensors ◽

10.3390/s19092067 ◽

2019 ◽

Vol 19 (9) ◽

pp. 2067 ◽

Cited By ~ 3

Author(s):

Yuhki Yanase ◽

Kyohei Yoshizaki ◽

Kaiken Kimura ◽

Tomoko Kawaguchi ◽

Michihiro Hide ◽

...

Keyword(s):

Real Time ◽

Clinical Diagnosis ◽

Living Cell ◽

Living Cells ◽

Sensor Chip ◽

Type I ◽

Label Free ◽

Cell Functions ◽

Spr Imaging ◽

Impedance Sensor

Label-free evaluation and monitoring of living cell conditions or functions by means of chemical and/or physical sensors in a real-time manner are increasingly desired in the field of basic research of cells and clinical diagnosis. In order to perform multi-parametric analysis of living cells on a chip, we here developed a surface plasmon resonance (SPR) imaging (SPRI)-impedance sensor that can detect both refractive index (RI) and impedance changes on a sensor chip with comb-shaped electrodes. We then investigated the potential of the sensor for label-free and real-time analysis of living cell reactions in response to stimuli. We cultured rat basophilic leukemia (RBL)-2H3 cells on the sensor chip, which was a glass slide coated with comb-shaped electrodes, and detected activation of RBL-2H3 cells, such as degranulation and morphological changes, in response to a dinitro-phenol-conjugated human serum albumin (DNP-HSA) antigen. Moreover, impedance analysis revealed that the changes of impedance derived from RBL-2H3 cell activation appeared in the range of 1 kHz–1 MHz. Furthermore, we monitored living cell-derived RI and impedance changes simultaneously on a sensor chip using the SPRI-impedance sensor. Thus, we developed a new technique to monitor both impedance and RI derived from living cells by using a comb-shaped electrode sensor chip. This technique may enable us to clarify complex living cell functions which affect the RI and impedance and apply this to medical applications, such as accurate clinical diagnosis of type I allergy.

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Label‐free in situ pH monitoring in a single living cell using an optical nanoprobe

Medical Devices & Sensors ◽

10.1002/mds3.10079 ◽

2020 ◽

Vol 3 (3) ◽

Cited By ~ 1

Author(s):

Qingbo Yang ◽

Xiaobei Zhang ◽

Yang Song ◽

Ke Li ◽

Honglan Shi ◽

...

Keyword(s):

Living Cell ◽

Ph Monitoring ◽

Label Free ◽

Single Living Cell ◽

Single Living

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A NANO-PLASMONIC MICROFLUIDIC BIOSENSOR FOR LABEL-FREE MONITORING OF LIVING CELL RESPONSE TO CHEMICALS

10.3390/optofluidics2017-04204 ◽

2017 ◽

Author(s):

Xudong Fan ◽

Wenhui Wang ◽

Xuzhou Li ◽

Long Tu ◽

Xiaotian Tan ◽

...

Keyword(s):

Cell Response ◽

Living Cell ◽

Label Free ◽

Microfluidic Biosensor

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Label free potentiometric sialic acid detection applicable to living cell diagnosis

2009 IEEE Sensors ◽

10.1109/icsens.2009.5398387 ◽

2009 ◽

Author(s):

Akira Matumoto ◽

Naoko Sato ◽

Horacio Cabral ◽

Kazunori Kataoka ◽

Yuji Miyahara

Keyword(s):

Sialic Acid ◽

Living Cell ◽

Label Free

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Solving the mechanisms responsible for organelle behavior in vivo by same-cell correlative light and electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100120473 ◽

1992 ◽

Vol 50 (1) ◽

pp. 14-15

Author(s):

Conly L. Rieder

Keyword(s):

Electron Microscopy ◽

Quantitative Analysis ◽

Light Microscopy ◽

Living Cell ◽

Light And Electron Microscopy ◽

Time Behavior ◽

Dynamic Interactions ◽

Positive Identification ◽

Cellular Components

The behavior of many cellular components, and their dynamic interactions, can be characterized in the living cell with considerable spatial and temporal resolution by video-enhanced light microscopy (video-LM). Indeed, under the appropriate conditions video-LM can be used to determine the real-time behavior of organelles ≤ 25-nm in diameter (e.g., individual microtubules—see). However, when pushed to its limit the structures and components observed within the cell by video-LM cannot be resolved nor necessarily even identified, only detected. Positive identification and a quantitative analysis often requires the corresponding electron microcopy (EM).

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Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

Essays in Biochemistry ◽

10.1042/ebc20200023 ◽

2020 ◽

Author(s):

Nikolas Hundt

Keyword(s):

Single Molecule ◽

Cellular Systems ◽

Single Molecule Imaging ◽

Label Free ◽

Measurement Sensitivity ◽

Mass Distributions ◽

Quantitative Measurements ◽

Contrast Mechanism ◽

Cellular Components ◽

Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

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A microfluidic device with integrated impedance detection for λ-DNA

MRS Proceedings ◽

10.1557/proc-773-n6.5 ◽

2003 ◽

Vol 773 ◽

Cited By ~ 1

Author(s):

Myung-Il Park ◽

Jonging Hong ◽

Dae Sung Yoon ◽

Chong-Ook Park ◽

Geunbae Im

Keyword(s):

Microfluidic Device ◽

Electrochemical Impedance ◽

Direct Detection ◽

Full Potential ◽

Label Free ◽

Buffer Solution ◽

Detection Systems ◽

Significant Difference ◽

Detection Of Biomolecules ◽

Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

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