Fluorescence Intensity Fluctuation Analysis of Receptor Oligomerization in Membrane Domains

2021 ◽  
Author(s):  
Gabriel Biener ◽  
Michael R. Stoneman ◽  
Valerică Raicu
Keyword(s):  
Fluorescence Intensity ◽  
Intensity Fluctuation ◽  
Fluctuation Analysis ◽  
Membrane Domains ◽  
Receptor Oligomerization

scholarly journals In Situ Measurements of Oligomerization State of NBCe1-A in Rat Kidneys Via Spatial Fluorescence Intensity Fluctuation Analysis

2010 ◽  
Vol 98 (3) ◽  
pp. 575a-576a
Author(s):  
Mikhail Sergeev ◽  
Antoine G. Godin ◽  
Liyo Kao ◽  
Natalia Abuladze ◽  
Ira Kurtz ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Intensity Fluctuation ◽  
In Situ Measurements ◽  
Fluctuation Analysis ◽  
Rat Kidneys

scholarly journals Effects of Oversampling and Scanning Artifacts on the Accuracy of Spatial Fluorescence Intensity Fluctuation Analysis Methods

2009 ◽  
Vol 96 (3) ◽  
pp. 34a
Author(s):  
Mikhail Sergeev ◽  
Antoine G. Godin ◽  
Jody L. Swift ◽  
Yves De Koninck ◽  
Paul W. Wiseman
Keyword(s):  
Fluorescence Intensity ◽  
Intensity Fluctuation ◽  
Fluctuation Analysis ◽  
Analysis Methods

scholarly journals Determination of Membrane Protein Transporter Oligomerization in Native Tissue Using Spatial Fluorescence Intensity Fluctuation Analysis

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e36215 ◽  
Author(s):  
Mikhail Sergeev ◽  
Antoine G. Godin ◽  
Liyo Kao ◽  
Natalia Abuladze ◽  
Paul W. Wiseman ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Fluorescence Intensity ◽  
Intensity Fluctuation ◽  
Fluctuation Analysis ◽  
Native Tissue

scholarly journals In Situ Measurement of Oligomerization State of NBCe1‐A in Kidney Tissues using Spatial Fluorescence Intensity Fluctuation Analysis

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Mikhail Sergeev ◽  
Antoine G Godin ◽  
Liyo Kao ◽  
Natalia Abuladze ◽  
Paul W Wiseman ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Intensity Fluctuation ◽  
In Situ Measurement ◽  
Fluctuation Analysis

Probing single-molecule electron–hole transfer dynamics at a molecule–NiO semiconductor nanocrystalline interface

2017 ◽  
Vol 19 (26) ◽  
pp. 17216-17223 ◽  
Author(s):  
Bharat Dhital ◽  
Vishal Govind Rao ◽  
H. Peter Lu
Keyword(s):  
Single Molecule ◽  
Fluorescence Intensity ◽  
Intensity Fluctuation ◽  
Hole Injection ◽  
Single Molecule Fluorescence ◽  
Electron Hole ◽  
Hole Transfer ◽  
Recombination Mechanism

We probe electron–hole injection as well as a recombination mechanism and dynamics at a dye–NiO interface based on single-molecule fluorescence intensity fluctuation and blinking measurements.

scholarly journals Reply To: Molecular Brightness analysis of GPCR oligomerization in the presence of spatial heterogeneity

2019 ◽  
Author(s):  
Michael R. Stoneman ◽  
Gabriel Biener ◽  
Valerică Raicu
Keyword(s):  
Spatial Heterogeneity ◽  
Fluorescence Intensity ◽  
Intensity Fluctuation ◽  
Regions Of Interest ◽  
Cellular Structures ◽  
Two Dimensional ◽  
Analysis Process ◽  
Brightness Analysis ◽  
Molecular Brightness ◽  
Selection Of

Annibale and Lohse have recently suggested a way1 in which the two-dimensional fluorescence intensity fluctuation (2D FIF) spectrometry2 may be further refined. Their main suggestion is to include a step in the analysis process where a case-by-case inspection of individual regions of interest of a membrane allows for selection of portions of the membrane which are “as homogenous as possible” and thereby exclude intensity spots potentially related to other sub-cellular structures. By incorporating that proposal into an objective and reproducible algorithm, here we show that 2D FIF has a built-in capability to automatically filter out such contributions, and that further removal of inhomogeneities does not alter the final results.

Intensity fluctuation analysis of cell scattering/imaging with clinical application

2007 ◽  
Author(s):  
Raji Subramaniam ◽  
R. Sullivan ◽  
P. S. Schneider ◽  
T. Holden ◽  
G. Tremberger, Jr. ◽  
...  
Keyword(s):  
Clinical Application ◽  
Intensity Fluctuation ◽  
Fluctuation Analysis ◽  
Cell Scattering
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