Two nondimensional parameters for characterizing the nuclear morphology

2021 ◽  
Author(s):  
Sreenath Balakrishnan ◽  
Shilpa.R. Raju ◽  
Anwesha Barua ◽  
Reshma P. Pradeep ◽  
G.K. Ananthasuresh
Keyword(s):  
Nuclear Morphology

An adrenocortical oncocytoma with unusual mitochondrial inclusions and cytoplasmic crystals

1994 ◽  
Vol 52 ◽  
pp. 250-251
Author(s):  
W.T. Gunning ◽  
G.D. Haselhuhn ◽  
E.R. Phillips ◽  
S.H. Selman
Keyword(s):  
Adrenal Gland ◽  
Salivary Glands ◽  
Mitotic Activity ◽  
Diagnostic Criteria ◽  
Vascular Invasion ◽  
Benign Neoplasm ◽  
Benign Tumors ◽  
Nuclear Morphology ◽  
Case Presentation ◽  
Microscopic Evidence

Within the last few years, adrenal cortical tumors with features concordant with the diagnostic criteria attributed to oncocytomas have been reported. To date, only nine reported cases exist in the literature. This report is the tenth case presentation of a presumptively benign neoplasm of the adrenal gland with a rare differentiation. Oncocytomas are well recognized benign tumors of the thyroid, parathyroid, and salivary glands and of the kidney. Other organs also give rise to these types of tumors, however with less frequency than the former sites. The characteristics generally used to classify a tumor as an oncocytoma include the following criteria: the tumor is 1) usually a solitary circumscribed mass with no gross nor microscopic evidence of metastasis (no tissue nor vascular invasion), 2) fairly bland in terms of mitotic activity and nuclear morphology, and 3) composed of large eosinophillic cells in which the cytoplasm is packed full of mitochondria (Figure 1).

scholarly journals Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna

2021 ◽  
Author(s):  
Rhayane Alves Assis ◽  
Wadson Rodrigues Rezende ◽  
Cirley Gomes Araújo dos Santos ◽  
Marcelino Benvindo-Souza ◽  
Nathan Pereira Lima Amorim ◽  
...  
Keyword(s):  
Nuclear Morphology ◽  
Brazilian Cerrado ◽  
Habitat Differences ◽  
Cerrado Savanna

Testosterone exposure in prenatal life disrupts epithelial nuclear morphology, smooth muscle layer pattern, and FGF10 and Shh expression in prostate

Life Sciences ◽  
2021 ◽  
Vol 271 ◽  
pp. 119198
Author(s):  
Luana Araújo Manso ◽  
Barbara Costa Malmann Medeiros ◽  
Giovanna Amaral Rodrigues ◽  
Jordana Gomes Ramos ◽  
Mara Rúbia Marques ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Layer ◽  
Nuclear Morphology ◽  
Smooth Muscle Layer

Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears

2020 ◽  
Vol 64 (4) ◽  
pp. 375-377
Author(s):  
Ekkehard Hewer ◽  
Anja M. Schmitt
Keyword(s):  
Organic Solvents ◽  
Toluidine Blue ◽  
Critical Factor ◽  
Blue Staining ◽  
Nuclear Morphology ◽  
Hazardous Chemicals ◽  
Toluidine Blue Staining ◽  
Site Evaluation ◽  
The Rose

Rapid on-site evaluation (ROSE) is one of cytopathology’s “unique selling propositions.” The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.

Insulator foci distance correlates with cellular and nuclear morphology in early Drosophila embryos

2021 ◽  
Vol 476 ◽  
pp. 189-199
Author(s):  
Mo Li ◽  
Qing Zhao ◽  
Ryan Belloli ◽  
Carly R. Duffy ◽  
Haini N. Cai
Keyword(s):  
Nuclear Morphology ◽  
Drosophila Embryos

scholarly journals Quantitative parameters of lymphocyte nuclear morphology in bronchoalveolar lavage fluid as novel biomarkers for sarcoidosis

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Yasushi Horimasu ◽  
Kakuhiro Yamaguchi ◽  
Shinjiro Sakamoto ◽  
Takeshi Masuda ◽  
Shintaro Miyamoto ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Bronchoalveolar Lavage ◽  
Validation Cohort ◽  
Lavage Fluid ◽  
Interstitial Lung Diseases ◽  
Nuclear Morphology ◽  
Small Areas ◽  
Quantitative Parameters ◽  
Novel Biomarkers ◽  
Lymphocyte Fraction

Abstract Background Bronchoalveolar lavage (BAL) is one of the fundamental examinations for the differential diagnosis of interstitial lung diseases (ILDs), and lymphocytosis strongly indicates alternative diagnoses rather than idiopathic pulmonary fibrosis. However, the BALF lymphocytosis is observed in several ILDs. We considered that quantitative evaluation of the BALF lymphocyte nuclear morphology would be useful in the differential diagnosis of ILDs with increased BALF lymphocyte fraction. Results One hundred and twenty-one patients with ILDs having increased BALF lymphocyte fraction were recruited (68 in the development cohort and 53 in the validation cohort). In the development cohort, BALF lymphocyte nuclei in sarcoidosis patients showed significantly smaller areas, shorter perimeters, lower radius ratios, and increased roundness than those of other ILD patients (p < 0.001 for each). Next, the fractions of lymphocytes with small areas, short perimeters, low radius ratios, and increased roundness, which were determined based on receiver operating characteristic (ROC) analyses-based thresholds, were demonstrated to be higher in sarcoidosis patients than in the other ILD patients (p < 0.001 for each). Furthermore, when we combined size-representing parameters with shape-representing parameters, the fraction of lymphocytes with small and round nuclei showed approximately 0.90 of area under the ROC curve in discriminating sarcoidosis both in the development cohort and the validation cohort. Conclusion This study is the first to demonstrate the usefulness of quantitative parameters of BALF lymphocyte nuclear morphology as novel biomarkers for sarcoidosis.

scholarly journals An Early and Robust Activation of Caspases Heads Cells for a Regulated Form of Necrotic-like Cell Death

2015 ◽  
Vol 290 (34) ◽  
pp. 20841-20855 ◽  
Author(s):  
Mercè Garcia-Belinchón ◽  
María Sánchez-Osuna ◽  
Laura Martínez-Escardó ◽  
Carla Granados-Colomina ◽  
Sònia Pascual-Guiral ◽  
...  
Keyword(s):  
Cell Death ◽  
Plasma Membrane ◽  
Chromatin Condensation ◽  
Biochemical Network ◽  
Display Type ◽  
Nuclear Morphology ◽  
Type Ii ◽  
Caspase Activation ◽  
Membrane Leakage ◽  
Nuclear Alterations

Apoptosis is triggered by the activation of caspases and characterized by chromatin condensation and nuclear fragmentation (type II nuclear morphology). Necrosis is depicted by a gain in cell volume (oncosis), swelling of organelles, plasma membrane leakage, and subsequent loss of intracellular contents. Although considered as different cell death entities, there is an overlap between apoptosis and necrosis. In this sense, mounting evidence suggests that both processes can be morphological expressions of a common biochemical network known as “apoptosis-necrosis continuum.” To gain insight into the events driving the apoptosis-necrosis continuum, apoptotically proficient cells were screened facing several apoptotic inducers for the absence of type II apoptotic nuclear morphologies. Chelerythrine was selected for further studies based on its cytotoxicity and the lack of apoptotic nuclear alterations. Chelerythrine triggered an early plasma membrane leakage without condensed chromatin aggregates. Ultrastructural analysis revealed that chelerythrine-mediated cytotoxicity was compatible with a necrotic-like type of cell death. Biochemically, chelerythrine induced the activation of caspases. Moreover, the inhibition of caspases prevented chelerythrine-triggered necrotic-like cell death. Compared with staurosporine, chelerythrine induced stronger caspase activation detectable at earlier times. After using a battery of chemicals, we found that high concentrations of thiolic antioxidants fully prevented chelerythrine-driven caspase activation and necrotic-like cell death. Lower amounts of thiolic antioxidants partially prevented chelerythrine-mediated cytotoxicity and allowed cells to display type II apoptotic nuclear morphology correlating with a delay in caspase-3 activation. Altogether, these data support that an early and pronounced activation of caspases can drive cells to undergo a form of necrotic-like regulated cell death.

Mutations in the gene encoding the lamin B receptor produce an altered nuclear morphology in granulocytes (Pelger–Huët anomaly)

10.1038/ng925 ◽  
2002 ◽  
Vol 31 (4) ◽  
pp. 410-414 ◽  
Author(s):  
Katrin Hoffmann ◽  
Christine K. Dreger ◽  
Ada L. Olins ◽  
Donald E. Olins ◽  
Leonard D. Shultz ◽  
...  
Keyword(s):  
Nuclear Morphology ◽  
Gene Encoding ◽  
Lamin B ◽  
Lamin B Receptor
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