Uterus and endometrium: Changes in nuclear morphology in the human endometrial glandular epithelium in women with unexplained infertility

SUMMARY [3H]Thymidine autoradiography was used to study DNA synthesis in the uteri of spayed mice treated with progesterone and oestradiol. Progesterone suppressed DNA synthesis in the glandular epithelium whether oestrogen was given or not. It also suppressed DNA synthesis in the luminal epithelium. Here oestradiol produced morphological changes and eventual re-entry of some cells into DNA synthesis. Progesterone altered the nuclear morphology of the stromal cells and increased the number synthesizing DNA. In these conditions a single injection of oestrogen was followed 10–15 h later by the synchronized entry into DNA synthesis of 30–40% of stromal cells. However, a second injection produced no further response. It was concluded that progesterone stimulated stromal cells in the resting phase to enter the cell cycle and that oestrogen then accelerated their passage through a single round of replication and division by shortening the interval between mitosis and DNA synthesis, following which the cells withdrew from the cell cycle.

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The fine structure of the human endometrial glandular epithelium in cases of unexplained infertility: a morphometric study

Human Reproduction ◽

10.1093/oxfordjournals.humrep.a138117 ◽

1993 ◽

Vol 8 (5) ◽

pp. 667-673 ◽

Cited By ~ 11

Author(s):

P. Dockery ◽

K. Pritchard ◽

A. Taylor ◽

T.C. Li ◽

M.A. Warren ◽

...

Keyword(s):

Fine Structure ◽

Unexplained Infertility ◽

Glandular Epithelium ◽

Morphometric Study

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An adrenocortical oncocytoma with unusual mitochondrial inclusions and cytoplasmic crystals

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100168980 ◽

1994 ◽

Vol 52 ◽

pp. 250-251

Author(s):

W.T. Gunning ◽

G.D. Haselhuhn ◽

E.R. Phillips ◽

S.H. Selman

Keyword(s):

Adrenal Gland ◽

Salivary Glands ◽

Mitotic Activity ◽

Diagnostic Criteria ◽

Vascular Invasion ◽

Benign Neoplasm ◽

Benign Tumors ◽

Nuclear Morphology ◽

Case Presentation ◽

Microscopic Evidence

Within the last few years, adrenal cortical tumors with features concordant with the diagnostic criteria attributed to oncocytomas have been reported. To date, only nine reported cases exist in the literature. This report is the tenth case presentation of a presumptively benign neoplasm of the adrenal gland with a rare differentiation. Oncocytomas are well recognized benign tumors of the thyroid, parathyroid, and salivary glands and of the kidney. Other organs also give rise to these types of tumors, however with less frequency than the former sites. The characteristics generally used to classify a tumor as an oncocytoma include the following criteria: the tumor is 1) usually a solitary circumscribed mass with no gross nor microscopic evidence of metastasis (no tissue nor vascular invasion), 2) fairly bland in terms of mitotic activity and nuclear morphology, and 3) composed of large eosinophillic cells in which the cytoplasm is packed full of mitochondria (Figure 1).

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Progestation

Acta Endocrinologica ◽

10.1530/acta.0.056s007 ◽

1967 ◽

Vol 56 (3_Suppla) ◽

pp. S7-S45 ◽

Cited By ~ 1

Keyword(s):

Tritiated Thymidine ◽

Succinic Dehydrogenase ◽

Glandular Epithelium ◽

Synthetic Activity ◽

Cell Renewal ◽

Pulse Labelling ◽

Uterine Receptivity ◽

Alkaline Phosphatases ◽

Monocytic Cells ◽

Acid And Alkaline Phosphatases

ABSTRACT Autoradiographic, enzymic and histologic studies on uteri of pregnant rats were carried out to follow the endometrial modifications which take place during progestation (days L0 – L4) and culminate in the state of uterine receptivity essential for ovum-implantation. Pulse labelling with tritiated thymidine (radioactive DNA precursor) on L0, L1 and L2 revealed a sequence of cell renewal in luminal and glandular epithelium and endometrial stroma. On L3 and L4 stromal cells showed extensive incorporation of tritiated thymidine. This synthetic activity was associated with endometrial preparation for decidualization and was evoked at least in part, by the surge of oestrogen on L3. All layers of the uterine wall were heavily infiltrated on L0 and resembled the site of an acute inflammatory reaction. Subsequently, polymorphonuclear infiltration diminished and monocytic cells predominated. On L3 a spatial arrangement was observed: eosinophiles were concentrated in the basal endometrium and monocytic cells in the subepithelial stroma. A comparison was made between such a shift in migratory cells in the uterus and similar phenomena which occur in inflammatory and immune reactions. Activities of acid and alkaline phosphatases, of ATP-ase and succinic dehydrogenase were low on L0 and L1 during the periods of infiltration, degeneration and regeneration of luminal and glandular epithelium. Enzymic activities increased on the following days, (L3 and L4). Vascular dilation and engorgement and endometrial oedema were observed near the blastocysts on L4. Most blastocysts incorporated tritiated thymidine after 14.00 h on L4, but some showed uptake before loss of the zona which occurs usually between 14.00 and 16.00 h; therefore, it was assumed that the permeability of the zona increases prior to being shed. Activities of succinic dehydrogenase and acid and alkaline phosphatases were demonstrable in blastocysts on L4 while they were still »free« in the uterine lumen.

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