Suppression of cell-cell variation by cooperative interaction of phosphatase and response regulator

2021 ◽  
Author(s):  
Xiang Liu ◽  
Rongjing Zhang ◽  
Junhua Yuan
Keyword(s):  
Response Regulator ◽  
Cooperative Interaction ◽  
Cell Variation ◽  
Cell Cell

scholarly journals Citrus limonoids interfere with Vibrio harveyi cell–cell signalling and biofilm formation by modulating the response regulator LuxO

Microbiology ◽  
2011 ◽  
Vol 157 (1) ◽  
pp. 99-110 ◽  
Author(s):  
Amit Vikram ◽  
Palmy R. Jesudhasan ◽  
G. K. Jayaprakasha ◽  
Suresh D. Pillai ◽  
Bhimanagouda S. Patil
Keyword(s):  
Biofilm Formation ◽  
Furan Ring ◽  
Insect Pests ◽  
Vibrio Harveyi ◽  
Response Regulator ◽  
Cell Signalling ◽  
Plant Defence ◽  
Significant Inhibition ◽  
Regulator Gene ◽  
Cell Cell

Citrus limonoids are unique secondary metabolites, characterized by a triterpenoid skeleton with a furan ring. Studies have demonstrated beneficial health properties of limonoids. In addition, certain citrus limonoids play a role in plant defence against insect pests. In the present study, five limonoids were purified from sour orange and evaluated for their ability to inhibit cell–cell signalling. The purified limonoids were tested for their ability to interfere with cell–cell signalling and biofilm formation in Vibrio harveyi. Isolimonic acid, deacetylnomilinic acid glucoside and ichangin demonstrated significant inhibition of autoinducer-mediated cell–cell signalling and biofilm formation. Furthermore, isolimonic acid and ichangin treatment resulted in induced expression of the response regulator gene luxO. In addition, luxR promoter activity was not affected by isolimonic acid or ichangin. Therefore, the ability of isolimonic acid and ichangin to interfere with cell–cell signalling and biofilm formation seems to stem from the modulation of luxO expression. The results suggest that isolimonic acid and ichangin are potent modulators of bacterial cell–cell signalling.

scholarly journals Robustness in an Ultrasensitive Motor

mBio ◽  
2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Guangzhe Liu ◽  
Antai Tao ◽  
Rongjing Zhang ◽  
Junhua Yuan
Keyword(s):  
Escherichia Coli ◽  
Response Regulator ◽  
Motor Output ◽  
Flagellar Motor ◽  
Content Type ◽  
P Concentration ◽  
Bacterial Flagellar Motor ◽  
Cell Variation

ABSTRACT In Escherichia coli, the chemotaxis response regulator CheY-P binds to FliM, a component of the switch complex at the base of the bacterial flagellar motor, to modulate the direction of motor rotation. The bacterial flagellar motor is ultrasensitive to the concentration of unbound CheY-P in the cytoplasm. CheY-P binds to FliM molecules both in the cytoplasm and on the motor. As the concentration of FliM unavoidably varies from cell to cell, leading to a variation of unbound CheY-P concentration in the cytoplasm, this raises the question whether the flagellar motor is robust against this variation, that is, whether the rotational bias of the motor is more or less constant as the concentration of FliM varies. Here, we showed that the motor is robust against variations of the concentration of FliM. We identified adaptive remodeling of the motor as the mechanism for this robustness. As the level of FliM molecules changes, resulting in different amounts of the unbound CheY-P molecules, the motor adaptively changes the composition of its switch complex to compensate for this effect. IMPORTANCE The bacterial flagellar motor is an ultrasensitive motor. Its output, the probability of the motor turning clockwise, depends sensitively on the occupancy of the protein FliM (a component on the switch complex of the motor) by the input CheY-P molecules. With a limited cellular pool of CheY-P molecules, cell-to-cell variation of the FliM level would lead to large unwanted variation of the motor output if not compensated. Here, we showed that the motor output is robust against the variation of FliM level and identified the adaptive remodeling of the motor switch complex as the mechanism for this robustness.

scholarly journals cgCorrect: a method to correct for confounding cell–cell variation due to cell growth in single-cell transcriptomics

2017 ◽  
Vol 14 (3) ◽  
pp. 036001 ◽  
Author(s):  
Thomas Blasi ◽  
Florian Buettner ◽  
Michael K Strasser ◽  
Carsten Marr ◽  
Fabian J Theis
Keyword(s):  
Cell Growth ◽  
Single Cell ◽  
Cell Variation ◽  
Cell Cell

scholarly journals Identification of a response regulator involved in surface attachment, cell-cell aggregation, exopolysaccharide production and virulence in the plant pathogenXylella fastidiosa

2012 ◽  
Vol 14 (3) ◽  
pp. 256-264 ◽  
Author(s):  
Tanja M. Voegel ◽  
Harshavardhan Doddapaneni ◽  
Davis W. Cheng ◽  
Hong Lin ◽  
Drake C. Stenger ◽  
...  
Keyword(s):  
Response Regulator ◽  
Cell Aggregation ◽  
Surface Attachment ◽  
Exopolysaccharide Production ◽  
Cell Cell

Application of Spurr, LX112, LX112/ARALDITE 502, POLY/BED 812 and Effapoxy Embedding Media to in Vitro Agar-Cultured Bone Marrow Colonies.

1980 ◽  
Vol 38 ◽  
pp. 646-647
Author(s):  
Glenn M. Buchanan ◽  
Dennis A. Stewart
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Cell Cell

In vitro bone-marrow derived colonies cultured in agar and prepared in Epon 812 for electron microscopy occassionally produce blocks that are too soft for sectioning. We attribute this softness to the retention, after standard dehydration, of water by the agar and to the relatively slow penetration of the agar by Epon-based embedding media. The agar cannot be removed or replaced since this would disrupt the colony integrity and prevent the study of cell-cell relationships. This paper describes the procedures and results of more extensive specimen dehydration and of embedding with Epon-replacement formulations.

622: Von Hippel-Lindau Inactivation Downregulates the Cell-Cell Adhesion Molecule E Cadherin in Renal Cancer Cells

2005 ◽  
Vol 173 (4S) ◽  
pp. 170-170
Author(s):  
Maxine G. Tran ◽  
Miguel A. Esteban ◽  
Peter D. Hill ◽  
Ashish Chandra ◽  
Tim S. O'Brien ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cancer Cells ◽  
Renal Cancer ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Von Hippel Lindau ◽  
E Cadherin ◽  
Cell Cell

Characterization of N-cadherin-containing cell-cell contacts of the liver

2006 ◽  
Vol 44 (01) ◽  
Author(s):  
BK Straub ◽  
J Boda-Heggemann ◽  
UF Pape ◽  
C Grund ◽  
E Specht-Delius ◽  
...  
Keyword(s):  
Cell Contacts ◽  
Cell Cell
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