Hyperbaric oxygen preconditioning protects cortical neurons against oxygen-glucose deprivation injury: Role of peroxisome proliferator-activated receptor-gamma

2012 ◽  
Vol 1452 ◽  
pp. 140-150 ◽  
Author(s):  
Yi Zeng ◽  
Keliang Xie ◽  
Hailong Dong ◽  
Haopeng Zhang ◽  
Feng Wang ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Cortical Neurons ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Hyperbaric Oxygen Preconditioning

Oxygen-glucose deprivation and reoxygenation on human cerebral organoids alters expression related to lipid metabolism

2020 ◽  
Author(s):  
Naoki Iwasa ◽  
Takeshi K. Matsui ◽  
Naritaka Morikawa ◽  
Yoshihiko M. Sakaguchi ◽  
Tomo Shiota ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Peroxisome Proliferator ◽  
Cerebral Tissue ◽  
Peroxisome Proliferator Activated Receptor ◽  
Induced Pluripotent ◽  
Effect Of Oxygen ◽  
Relationship Of ◽  
The Impact

AbstractIschemic stroke is one of the most common neurological disease. However, the impact of ischemic stroke on human cerebral tissue remains largely unknown; due to a lack of ischemic human brain samples. In this study, we used cerebral organoids derived from human induced pluripotent stem cells to evaluate the effect of oxygen-glucose deprivation/reoxygenation (OGD/R). We identified 15 differentially expressed genes (DEGs); and found that all the DEGs were downregulated. Pathway analysis showed the relationship of vitamin digestion and absorption, fat digestion and absorption, peroxisome proliferator-activated receptor signaling pathway, and complement and coagulation cascades. These findings indicate the mechanisms underlying ischemic injury in human cerebral tissue.

scholarly journals Sirt1 Mediates Hyperbaric Oxygen Preconditioning-Induced Ischemic Tolerance in Rat Brain

2013 ◽  
Vol 33 (3) ◽  
pp. 396-406 ◽  
Author(s):  
Wenjun Yan ◽  
Zongping Fang ◽  
Qianzi Yang ◽  
Hailong Dong ◽  
Yan Lu ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Cortical Neurons ◽  
Infarct Volume ◽  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
B Cell Lymphoma ◽  
Glucose Deprivation ◽  
Artery Occlusion ◽  
Class Iii ◽  
Hyperbaric Oxygen Preconditioning

Our previous studies have shown that hyperbaric oxygen preconditioning (HBO-PC) induces tolerance to cerebral ischemia/reperfusion (I/R). This study aimed to investigate whether SirT1, a class III histone deacetylase, is involved in neuroprotection elicited by HBO-PC in animal and cell culture models of ischemia. Rats were subjected to middle cerebral artery occlusion for 120 minutes after HBO-PC (once a day for 5 days). Primary cultured cortical neurons were exposed to 2 hours of HBO-PC after 2 hours of oxygen–glucose deprivation (OGD). We showed that HBO-PC increased SirT1 protein and mRNA expression, promoted neurobehavioral score, reduced infarct volume, and improved morphology at 24 hours and 7 days after cerebral I/R. Neuroprotection of HBO-PC was attenuated by SirT1 inhibitor EX527 and SirT1 knockdown by short interfering RNA (siRNA), whereas it was mimicked by SirT1 activator resveratrol. Furthermore, HBO-PC enhanced SirT1 expression and cell viability and reduced lactate dehydrogenase release 24 hours after OGD/re-oxygenation. The neuroprotective effect of HBO-PC was emulated through upregulating SirT1 and, reversely, attenuated through downregulating SirT1. The modulation of SirT1 was made by adenovirus infection carrying SirT1 or SirT1 siRNA. Besides, SirT1 increased B-cell lymphoma 2 (Bcl-2) expression and decrease cleaved caspase 3. These results indicate that SirT1 mediates HBO-PC-induced tolerance to cerebral I/R through inhibition of apoptosis.

Major role of the PI3K/Akt pathway in ischemic tolerance induced by sublethal oxygen-glucose deprivation in cortical neurons in vitro

2011 ◽  
Vol 34 (6) ◽  
pp. 1023-1034 ◽  
Author(s):  
Mohammad Iqbal Hossain Bhuiyan ◽  
Seo Yun Jung ◽  
Hyoung Ja Kim ◽  
Yong Sup Lee ◽  
Changbae Jin
Keyword(s):  
Cortical Neurons ◽  
Glucose Deprivation ◽  
Ischemic Tolerance ◽  
Akt Pathway ◽  
Oxygen Glucose Deprivation

scholarly journals Gene Expression Profiles of Human Cerebral Organoids Identify PPAR Pathway and PKM2 as Key Markers for Oxygen-Glucose Deprivation and Reoxygenation

2021 ◽  
Vol 15 ◽  
Author(s):  
Naoki Iwasa ◽  
Takeshi K. Matsui ◽  
Naohiko Iguchi ◽  
Kaoru Kinugawa ◽  
Naritaka Morikawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ischemic Stroke ◽  
Signaling Pathway ◽  
Expression Profiles ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Ppar Signaling ◽  
The Impact

Ischemic stroke is one of the most common neurological diseases. However, the impact of ischemic stroke on human cerebral tissue remains largely unknown due to a lack of ischemic human brain samples. In this study, we applied cerebral organoids derived from human induced pluripotent stem cells to evaluate the effect of oxygen-glucose deprivation/reoxygenation (OGD/R). Pathway analysis showed the relationships between vitamin digestion and absorption, fat digestion and absorption, peroxisome proliferator-activated receptor (PPAR) signaling pathway, and complement and coagulation cascades. Combinational verification with transcriptome and gene expression analysis of different cell types revealed fatty acids-related PPAR signaling pathway and pyruvate kinase isoform M2 (PKM2) as key markers of neuronal cells in response to OGD/R. These findings suggest that, although there remain some limitations to be improved, our ischemic stroke model using human cerebral organoids would be a potentially useful tool when combined with other conventional two-dimensional (2D) mono-culture systems.

Sign in / Sign up

Export Citation Format

Share Document