Chemoprevention of mammary carcinogenesis in female rats by rofecoxib

AbstractChemopreventive effect of non-steroidal antiinflammatory drugs (NSAIDs) in mammary carcinogenesis was reported in several studies. In this study, the effect of a nonselective cyclooxygenase inhibitor diclofenac (DICLO) in the prevention of N-methyl-N-nitrosourea (NMU)-induced mammary carcinogenesis in Sprague-Dawley female rats was evaluated. NMU was administered to animals intraperitoneally in two doses of 50 mg kg−1 b.w. within postnatal days 42-48. In experiment A (short-term administration), DICLO was administrated intramuscularly (5 mg kg−1 b.w.) every other day, starting 3 days before and for subsequent 25 days after first NMU injection. In experiment B (long-term administration), DICLO was administered in tap water (0.01 mg ml−1) continually, starting 7 days before and for subsequent 22 weeks after first NMU dose. The study was terminated 22 weeks after the first dose of NMU in both experiments. After DICLO treatment, tumor frequency per group was reduced in both variants of drug administration: in experiment A by 38% and in experiment B by 39.5%. Moreover, DICLO decreased tumor incidence by 11.5% and delayed tumor latency by 14 days in experiment B. In our preventive-curative experiments DICLO decreased some parameters of NMU-induced rat mammary carcinogenesis, mainly the tumor frequency.

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Lipotrope‐modified diets enhance nitrosomethylurea‐induced mammary carcinogenesis in female rats

Nutrition and Cancer ◽

10.1080/01635589309514289 ◽

1993 ◽

Vol 20 (3) ◽

pp. 215-221 ◽

Cited By ~ 7

Author(s):

Chang B. Choi ◽

Myung G. Baik ◽

Wanda L. Keller ◽

Chung S. Park

Keyword(s):

Mammary Carcinogenesis ◽

Female Rats

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Retinyl Acetate and Melatonin in Chemoprevention of Mammary Carcinogenesis in Female Rats: Metabolic Changes

Acta Veterinaria Brno ◽

10.2754/avb200170010057 ◽

2001 ◽

Vol 70 (1) ◽

pp. 57-63

Author(s):

M. Chamilová ◽

B. Bojková ◽

K. Kalická ◽

P. Kubatka ◽

E. Adámeková ◽

...

Keyword(s):

Mammary Carcinogenesis ◽

Metabolic Changes ◽

Female Rats ◽

Retinyl Acetate

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Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene

Revista do Hospital das Clínicas ◽

10.1590/s0041-87812004000500006 ◽

2004 ◽

Vol 59 (5) ◽

pp. 257-261 ◽

Cited By ~ 38

Author(s):

Alfredo Carlos S. D. Barros ◽

Elisa Naomi K. Muranaka ◽

Lincon Jo Mori ◽

Christina Helena T. Pelizon ◽

Kyoshi Iriya ◽

...

Keyword(s):

Animal Model ◽

Light Microscopy ◽

Breast Tumor ◽

Mammary Carcinogenesis ◽

Mammary Glands ◽

Female Rats ◽

Histopathological Analysis ◽

Breast Carcinomas ◽

Sprague Dawley

PURPOSE: To test an experimental model of chemical mammary carcinogenesis induction in rats. METHODS: Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy. RESULTS: Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis. CONCLUSION: This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.

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Maternal Western Style Diet Increases Susceptibility to Chemically-Induced Mammary Carcinogenesis in Female Rats Offspring

Nutrition and Cancer ◽

10.1080/01635581.2014.956256 ◽

2014 ◽

Vol 66 (8) ◽

pp. 1293-1303 ◽

Cited By ~ 2

Author(s):

Gisele A. D. Lopes ◽

William Y. C. Fan ◽

Heloisa Ciol ◽

Lucas T. Bidinotto ◽

Maria A. M. Rodrigues ◽

...

Keyword(s):

Mammary Carcinogenesis ◽

Female Rats ◽

Chemically Induced

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No effect of a high-fat diet on promotion of sex hormone-induced prostate and mammary carcinogenesis in the Noble rat model

British Journal Of Nutrition ◽

10.1079/bjn2002673 ◽

2002 ◽

Vol 88 (4) ◽

pp. 399-409 ◽

Cited By ~ 10

Author(s):

G. Leung ◽

I. F. F. Benzie ◽

A. Cheung ◽

S. W. Tsao ◽

Y. C. Wong

Keyword(s):

Weight Gain ◽

Rat Model ◽

Dietary Fat ◽

High Fat Diet ◽

Mammary Cancer ◽

Mammary Carcinogenesis ◽

Sex Hormone ◽

Female Rats ◽

Male Rats ◽

High Fat

Results of international correlation and migrant studies suggest that dietary fat promotes carcinogenesis in hormone-sensitive sites, but this is disputed. In the present study, we used a Noble rat model of sex hormone-induced cancers to examine the effect of a high-fat diet on the incidence and latency of prostate and mammary cancer in male (n 139) and female (n 72) animals respectively. We also measured α-tocopherol levels in female breast tissue to determine whether a high intake of polyunsaturated fatty acids depletes antioxidant defence in target tissues, providing a possible potentiating mechanism for carcinogenesis. Results showed a very high incidence of hormone-induced adenocarcinomas of prostate and mammary gland, irrespective of diet. There was no difference in the pattern of carcinogenesis in different prostatic locations, weight of the prostate, or weight gain between male rats on the high-fat diet compared with the control (standard, low-fat) diet. In female rats, the incidence of mammary cancer and the body-weight gain were the same in both dietary groups, and breast α-tocopherol was also unaffected by dietary fat intake. Our present results are supportive of recent cohort studies that reported no significant association between intake of fat and the development of human prostate and breast cancer, and do not support a role for dietary fat in promoting sex hormone-induced prostate and mammary carcinogenesis.

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Antitumor effects of atorvastatin in the chemoprevention of rat mammary carcinogenesis

Biologia ◽

10.2478/s11756-011-0077-3 ◽

2011 ◽

Vol 66 (4) ◽

Cited By ~ 8

Author(s):

Peter Kubatka ◽

Katarína Žihlavniková ◽

Peter Solár ◽

Karol Kajo ◽

Vanda Valentová ◽

...

Keyword(s):

Mrna Expression ◽

Latency Period ◽

Mammary Carcinogenesis ◽

Epidemiological Studies ◽

Suppressive Effect ◽

Female Rats ◽

Control Group ◽

Antitumor Effects ◽

Atorvastatin Treatment ◽

Reductase Inhibitors

AbstractEpidemiological studies indicate that 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors, or statins, play a role in inhibition of several human neoplasia including breast cancer. In this study, chemopreventive effects of atorvastatin in N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated. Atorvastatin was administered in the diet at two concentrations: 10 mg/kg (ATOR 10) and 100 mg/kg (ATOR 100). Atorvastatin treatment began 8 days prior to carcinogen administration and subsequently continued for 15 weeks till the end of the experiment. Atorvastatin at a higher dose suppressed tumor frequency by 80.5% (P = 0.0008) and tumor incidence by 49.5% (P = 0.015), and extended latency period by 14 days (P = 0.076) when compared to the control group. Atorvastatin at a lower dose did not significantly alter tumor parameters in comparison with the control group. In the specimens of mammary tumors, atorvastatin (in the ATOR 100 group) significantly decreased mRNA expression of Bcl-2 gene but non-significantly increased Bax mRNA expression compared to control group. Atorvastatin administration did not alter serum concentration of triacylglycerols, total cholesterol, and LDL cholesterol in comparison with controls. This study is the first report on tumor suppressive effect of atorvastatin in rat mammary carcinogenesis.

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