In this study, antibacterial activity and long-lasting release of silver for clinical applications were achieved by utilizing silver nanoparticles on the Titania nanotubes (TNTs) through in situ polymerization of polydopamine (PDA). TNTs were synthesized with the hydrothermal process from Titania nanoparticles. Then the surface modification of TNTs was accomplished by in situ polymerization of PDA and silver ions were reduced on the PDA surface. The feature of obtained samples was characterized using transmission electron microscope (TEM), field emission scanning electron microscope (FESEM), X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR) and atomic absorption spectrophotometry (AAS). The results showed that PDA layer formed on the synthesized anatase TNTs surface. This used as reduction agent for silver ions as well as an adhesive layer to tethering the silver nanoparticles on TNTs surface. AAS results indicated that silver ions reduction to silver nanoparticles on the TNTs surface increased from 3.1 wt.% to 9.6 wt.% in presence of PDA. Also, the results of silver release revealed that PDA worked as an adhesive layer by chelating silver nanoparticles on TNTs and slowing silver ions release rate which implying the possible long-term antibacterial activity of PDA coated TNTs. Besides that, TNTs showed 33% antibacterial activity which is half than silver loaded TNTs-PDA samples. This confirms that PDA have extraordinary effect on the antibacterial activity. This work offers a facile process for the preparation of long-lasting silver based antibacterial activity and facilitates their clinical application in the modern biomedical fields.