UROKINASE (U-PA) - INDUCED SMOOTH MUSCLE CELL (SMC) PROLIFERATION REQUIRES PLASMIN-DEPENDENT EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR) ACTIVATION

2004 ◽  
Vol 13 (3) ◽  
pp. 154
Author(s):  
Suzanne M Nicholl ◽  
Elisa Roztocil ◽  
Mark G Davies
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Growth Factor Receptor ◽  
Egfr Activation ◽  
Epidermal Growth

scholarly journals Nox1 transactivation of epidermal growth factor receptor promotes N-cadherin shedding and smooth muscle cell migration

2011 ◽  
Vol 93 (3) ◽  
pp. 406-413 ◽  
Author(s):  
Dammanahalli K. Jagadeesha ◽  
Maysam Takapoo ◽  
Botond Banfi ◽  
Ramesh C. Bhalla ◽  
Francis J. Miller
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Smooth Muscle ◽  
Cell Migration ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Growth Factor Receptor ◽  
Smooth Muscle Cell Migration ◽  
Epidermal Growth

T Cell–Induced Airway Smooth Muscle Cell Proliferation via the Epidermal Growth Factor Receptor

2013 ◽  
Vol 49 (4) ◽  
pp. 563-570 ◽  
Author(s):  
Saba Al Heialy ◽  
Paul-Andre Risse ◽  
Melissa A. Zeroual ◽  
Horia N. Roman ◽  
Kimitake Tsuchiya ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epidermal Growth Factor Receptor ◽  
Smooth Muscle ◽  
T Cell ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Smooth Muscle Cell ◽  
Growth Factor Receptor ◽  
Airway Smooth Muscle Cell ◽  
Epidermal Growth

Platelet-activating factor induces ovine fetal pulmonary venous smooth muscle cell proliferation: role of epidermal growth factor receptor transactivation

2007 ◽  
Vol 292 (6) ◽  
pp. H2773-H2781 ◽  
Author(s):  
Weilin Zhou ◽  
Basil O. Ibe ◽  
J. Usha Raj
Keyword(s):  
Cell Proliferation ◽  
Epidermal Growth Factor Receptor ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Smooth Muscle Cell ◽  
Platelet Activating Factor ◽  
Growth Factor Receptor ◽  
Epidermal Growth ◽  
Ovine Fetal

We have previously reported that platelet-activating factor (PAF) is present in very high levels in the ovine fetal lung and circulation and that PAF serves as an important physiological vasoconstrictor of the pulmonary circulation in utero. However, it is not known whether PAF stimulates pulmonary vascular smooth muscle cell (SMC) proliferation. In this study, we used ovine fetal pulmonary venous SMCs as our model system to study the effects and mechanisms of action of PAF on SMC proliferation. We found that PAF induced SMC proliferation in a dose-dependent manner. PAF also stimulated activation of both ERK and p38 but not c-Jun NH2 terminal kinase (JNK) mitogen-activated protein (MAP) kinase pathways. PAF (10 nM) induced phosphorylation of epidermal growth factor receptor (EGFR). Specific inhibition of EGFR by AG-1478 and by the expression of a dominant-negative EGFR mutant in SMCs attenuated PAF-stimulated cell proliferation. Inhibition of heparin-binding EGF-like growth factor (HB-EGF) release by CRM-197 and inhibition of matrix metalloproteinases (MMP) by GM-6001 abolished PAF-induced MAP kinase activation and cell proliferation. Increased alkaline phosphatase (AP) activity after PAF treatment in AP-HB-EGF fusion construct-transfected SMCs indicated that PAF induced the release of HB-EGF within 1 min. Gelatin zymography data showed that PAF stimulated MMP-2 activity and MMP-9 activity within 1 min. These results suggest that PAF promotes pulmonary vascular SMC proliferation via transactivation of EGFR through MMP activation and HB-EGF, resulting in p38 and ERK activation and that EGFR transactivation is essential for the mitogenic effect of PAF in pulmonary venous SMC.

Sign in / Sign up

Export Citation Format

Share Document