Effect of curcumin analog on γ-radiation-induced cellular changes in primary culture of isolated rat hepatocytes in vitro

2008 ◽  
Vol 176 (1) ◽  
pp. 1-8 ◽  
Author(s):  
M. Srinivasan ◽  
A. Ram Sudheer ◽  
K.N. Rajasekaran ◽  
Venugopal P. Menon
Keyword(s):  
Primary Culture ◽  
Rat Hepatocytes ◽  
Curcumin Analog ◽  
Isolated Rat Hepatocytes ◽  
Γ Radiation ◽  
Cellular Changes ◽  
Radiation Induced ◽  
Isolated Rat

Influence of ferulic acid on γ-radiation induced DNA damage, lipid peroxidation and antioxidant status in primary culture of isolated rat hepatocytes

Toxicology ◽  
2006 ◽  
Vol 228 (2-3) ◽  
pp. 249-258 ◽  
Author(s):  
M. Srinivasan ◽  
A. Ram Sudheer ◽  
K. Raveendran Pillai ◽  
P. Raghu Kumar ◽  
P.R. Sudhakaran ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Ferulic Acid ◽  
Primary Culture ◽  
Antioxidant Status ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Γ Radiation ◽  
Radiation Induced ◽  
Isolated Rat

Modulatory effects of curcumin on γ-radiation-induced cellular damage in primary culture of isolated rat hepatocytes

2007 ◽  
Vol 24 (2) ◽  
pp. 98-105 ◽  
Author(s):  
M. Srinivasan ◽  
A. Ram Sudheer ◽  
K. Raveendran Pillai ◽  
P. Raghu Kumar ◽  
P.R. Sudhakaran ◽  
...  
Keyword(s):  
Primary Culture ◽  
Rat Hepatocytes ◽  
Cellular Damage ◽  
Isolated Rat Hepatocytes ◽  
Γ Radiation ◽  
Radiation Induced ◽  
Isolated Rat

The Hep G2 Cell Line as a Possible Alternative to Isolated Hepatocytes in Cytotoxicity Studies

1988 ◽  
Vol 16 (1) ◽  
pp. 16-22
Author(s):  
Marina Marinovich ◽  
Jose L. Lorenzo ◽  
Liliana M. Flaminio ◽  
Agnese Granata ◽  
Corrado L. Galli
Keyword(s):  
Cell Line ◽  
Rat Hepatocytes ◽  
Prostaglandin E ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Isolated Rat Hepatocytes ◽  
G2 Cells ◽  
Isolated Rat

The hepatotoxicity of carbon tetrachloride (CC14) was evaluated in vitro in freshly isolated rat hepatocytes and in the human hepatoma cell line, Hep G2. Toxicity was assessed by the leakage of cytosolic enzymes (lactate dehydrogenase and aspartate aminotransferase) and cell viability (trypan blue exclusion). The established human cells were less sensitive to CCl4-induced injury; higher doses of the toxic agent and longer incubation times were necessary to elicit cell damage. Micromolar concentrations of prostaglandin E2 significantly decreased enzyme leakage in both Hep G2 cells and rat hepatocytes challenged with CC14; a stable derivative of prostacyclin (ZK 36374) was ineffective. These results suggest that human hepatoma Hep G2 cells may represent a valid alternative to isolated rat hepatocytes for an initial approach to the in vitro evaluation of cell toxicity.

Acellular liver matrix improves the survival and functions of isolated rat hepatocytes cultured in vitro.

2004 ◽  
Author(s):  
Patrizia Burra ◽  
Silvia Tomat ◽  
Maria Teresa Conconi ◽  
Carlo Macchi ◽  
Francesco P Russo ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Preparation of microgram quantities of BaP-DNA adducts using isolated rat hepatocytes in vitro

1989 ◽  
Vol 10 (4) ◽  
pp. 789-791 ◽  
Author(s):  
David A. Dankovic ◽  
David L. Springer ◽  
David B. Mann ◽  
Linda G. Smith ◽  
Berta L. Thomas ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals EFFECT OF MURRAYA KOENIGII LEAVES EXTRACT ON GLUCONEOGENESIS AND GLYCOGENOLYSIS IN ISOLATED RAT HEPATOCYTES CULTURE

2018 ◽  
Vol 11 (11) ◽  
pp. 264
Author(s):  
Rohan S Phatak ◽  
Chitra C Khanwelkar ◽  
Kailas D Datkhile ◽  
Pratik P Durgawale
Keyword(s):  
Glycogen Content ◽  
Ex Vivo ◽  
Rat Hepatocytes ◽  
Diabetic Rats ◽  
Murraya Koenigii ◽  
Isolated Rat Hepatocytes ◽  
Genes Expression ◽  
Different Doses ◽  
Isolated Rat

Objectives: The present study was aimed to investigate the in vitro activity of Murraya koenigii extracts through various carbohydrate metabolic pathways in the isolated rat hepatocyte models.Methods: Different doses of metformin, aqueous and methanol extracts of M. koenigii leaves were evaluated in the MTT, glucose, and glycogen content assays in the cultured in vitro rat hepatocytes.Results: The study showed that there was a significant increase in activity with respect to the increased concentration of extracts. Slight effect was observed in the isolated rat hepatocytes culture, M. koenigii leaves extract may exert cytoprotective and hypoglycemic action.Conclusion: It may be needed to determine the effect of ex vivo rat hepatocytes isolated from diabetic rats. Effects of the plant or isolated compounds on the genes expression of signaling pathways should be investigated in further studies.

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