Possible roles of gonadotropin-releasing hormone (GnRH) and melatonin in the control of gonadal development of clam Ruditapes philippinarum

Abstract Background: Gonadotropin releasing hormone (GnRH) plays an important role in the regulation of vertebrate reproduction. Studies have shown that immunization against GnRHa can induce sexually sterile tilapia. To explore the mechanism behind this, in this study, RNA-seq and data-independent acquisition (DIA) techniques were used to study the transcriptome and proteome of the gonad of tilapia immunized with GnRHa. Results : 644 differentially expressed genes (80 upregulated and 564 downregulated) and 1150 differentially expressed proteins (351 upregulated and 799 downregulated) were identified. There were 209 genes with consistent differential expression patterns in the transcriptomic and proteomic analyses, of which 9 were upregulated and 200 downregulated, indicating that the gonad gene expression was inhibited by GnRHa immunization. The downregulated genes were particularly involved in the functions of single-organism process, binding, cellular process, metabolic process and catalytic activity, and associated with the pathways including ECM–receptor interaction, focal adhesion, cardiac muscle contraction and oxidative phosphorylation. The expression of six differentially expressed genes involved in the GnRH signaling pathway was all downregulated. In addition, several important functional genes related to gonadal development after GnRHa immunization were screened. Conclusions: This study confirmed the expression of corresponding genes was affected by GnRHa on the gonad development in tilapia at the molecular level, and laid a foundation for elucidating the mechanism of GnRHa immunization.

Download Full-text

Molecular cloning, characterization, and expression analysis of a gonadotropin-releasing hormone-like cDNA in the clam, Ruditapes philippinarum

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpb.2015.07.005 ◽

2015 ◽

Vol 189 ◽

pp. 47-54 ◽

Cited By ~ 7

Author(s):

Ying Song ◽

Jingjing Miao ◽

Yuefeng Cai ◽

Luqing Pan

Keyword(s):

Molecular Cloning ◽

Expression Analysis ◽

Ruditapes Philippinarum ◽

Gonadotropin Releasing Hormone ◽

Releasing Hormone

Download Full-text

Seasonal fluctuations in pituitary levels of the three forms of gonadotropin-releasing hormone in striped bass, Morone saxatilis (Teleostei), during juvenile and pubertal development

Journal of Endocrinology ◽

10.1677/joe.0.1690527 ◽

2001 ◽

Vol 169 (3) ◽

pp. 527-538 ◽

Cited By ~ 44

Author(s):

MC Holland ◽

S Hassin ◽

Y Zohar

Keyword(s):

Striped Bass ◽

Gonadosomatic Index ◽

Pubertal Development ◽

Juvenile Fish ◽

Mammalian Species ◽

Gonadal Development ◽

Gonadotropin Releasing Hormone ◽

Oocyte Diameter ◽

Releasing Hormone ◽

Mature Fish

In most vertebrates, the development of a mature gonadotropin-releasing hormone (GnRH) secretory system is pivotal for the onset of puberty. The role of the three native GnRH forms, seabream (sb) GnRH, chicken (c) GnRH-II and salmon GnRH, in striped bass puberty remains elusive. This study examined the changes in pituitary GnRH levels throughout juvenile and pubertal development, a period encompassing 3 to 4 years. The levels of the two most abundant forms in the pituitary, sbGnRH and cGnRH-II (10:1), increased during the Fall and peaked prior to (cGnRH-II) or during (sbGnRH) the natural breeding season in March to May. In most cases, sbGnRH and cGnRH-II levels of maturing fish correlated to changes in oocyte diameter, gonadosomatic index and LH pituitary content. Interestingly, pituitaries of immature and maturing 2- and 3-year-old males and females contained similar amounts of all three GnRH forms. Additionally, pituitary sbGnRH and cGnRH-II levels in juvenile fish were relatively high and GnRH profiles showed a clear seasonality, similar to those of older, mature fish. These findings suggest a role for both sbGnRH and cGnRH-II in the regulation of gonadal development and indicate that, unlike some mammalian species, the timing of puberty in striped bass is not limited by a low activity of the GnRH system.

Download Full-text

Continuous Gonadotropin-Releasing Hormone Infusion Stimulates Dramatic Gonadal Development in Hypogonadal Female Mice1

Biology of Reproduction ◽

10.1095/biolreprod50.3.680 ◽

1994 ◽

Vol 50 (3) ◽

pp. 680-685 ◽

Cited By ~ 12

Author(s):

Marie J. Gibson ◽

Hahnah Kasowski ◽

Areta Dobrjansky

Keyword(s):

Gonadal Development ◽

Gonadotropin Releasing Hormone ◽

Releasing Hormone

Download Full-text

Seasonal Changes in Plasma Hormones, Sex-Related Genes Transcription in Brain, Liver and Ovary during Gonadal Development in Female Rainbow Trout (Oncorhynchus mykiss)

Fishes ◽

10.3390/fishes6040062 ◽

2021 ◽

Vol 6 (4) ◽

pp. 62

Author(s):

Huiqin Chen ◽

Baoliang Bi ◽

Lingfu Kong ◽

Hua Rong ◽

Yanhua Su ◽

...

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Seasonal Changes ◽

Gonadal Development ◽

Gonadotropin Releasing Hormone ◽

Endocrine Activity ◽

Continuous Increase ◽

Releasing Hormone ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Plasma Hormones

The purpose of this study was to investigate the periodic seasonal changes in endocrine activity and gonadal development of female rainbow trout (Oncorhynchus mykiss) in a high-altitude cold-water environment. The fish were sampled monthly from January to November and the levels of plasma hormones (estradiol (E2), cortisol and thyroid hormones (THS)) and vitellogenin (VTG) were measured by ELISA. Moreover, the transcriptions of sex-related genes in the ovary, brain, and liver were detected by qRT-PCR. The results showed a seasonal fluctuation of plasma hormones and VTG together with the development of the ovary, which reached a peak from August to October. Similarly, the transcription of hypothalamic gonadotropin-releasing hormone-2 (cgnrh-2), hypothalamic gonadotropin-releasing hormone receptors (gnrhr) and follicle-stimulating hormone (fsh) in the brain varied from January to September, but the highest level was detected in September to November. In addition, the transcription of sex-related genes located in the ovary and liver increased significantly during August to October, accompanied by a continuous increase in the gonadosomatic index (GSI) and a decrease in the hepatosomatic index (HSI). Therefore, plasma hormones and sex-related genes regulate the development and maturation of O. mykiss oocytes with the change in seasons and peaked in November. The results of this study provide a reference for improving the efficiency of the artificial reproduction of O. mykiss.

Download Full-text