Cloning and characterization of a riboflavin-binding hexamerin from the larval fat body of a lepidopteran stored grain pest, Corcyra cephalonica

2016 ◽  
Vol 194-195 ◽  
pp. 58-64 ◽  
Author(s):  
V. Venkat Rao ◽  
Thuirei Jacob Ningshen ◽  
R.K. Chaitanya ◽  
B. Senthilkumaran ◽  
Aparna Dutta-Gupta
Keyword(s):  
Fat Body ◽  
Corcyra Cephalonica ◽  
Stored Grain ◽  
Stored Grain Pest

Structural characterization of chitosan nanoparticle loaded with Piper nigrum essential oil for biological efficacy against the stored grain pest control

2020 ◽  
Vol 166 ◽  
pp. 104566 ◽  
Author(s):  
Vallavan Rajkumar ◽  
Chinappan Gunasekaran ◽  
Jayaraman Dharmaraj ◽  
Panneerselvam Chinnaraj ◽  
Cheruvathur Amita Paul ◽  
...  
Keyword(s):  
Essential Oil ◽  
Pest Control ◽  
Structural Characterization ◽  
Piper Nigrum ◽  
Stored Grain ◽  
Biological Efficacy ◽  
Chitosan Nanoparticle ◽  
Stored Grain Pest

20-Hydroxyecdysone regulation of H-fibroin gene in the stored grain pest Corcyra cephalonica, during the last instar larval development

Steroids ◽  
2011 ◽  
Vol 76 (1-2) ◽  
pp. 125-134 ◽  
Author(s):  
R.K. Chaitanya ◽  
P. Sridevi ◽  
B. Senthilkumaran ◽  
Aparna Dutta Gupta
Keyword(s):  
Larval Development ◽  
Corcyra Cephalonica ◽  
Stored Grain ◽  
Fibroin Gene ◽  
Stored Grain Pest

scholarly journals Purification and characterization of fat body lipase from the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

2019 ◽  
Vol 81 (1) ◽  
Author(s):  
Rahma R. Z. Mahdy ◽  
Shaimaa A. Mo’men ◽  
Marah M. Abd El-Bar ◽  
Emad M. S. Barakat
Keyword(s):  
Metal Ions ◽  
Kinetic Parameters ◽  
Biochemical Characterization ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Specific Activity ◽  
Larval Instar ◽  
Gel Filtration ◽  
Molecular Weights

Abstract Background Insect lipid mobilization and transport are currently under research, especially lipases and lipophorin because of their roles in the production of energy and lipid transport at a flying activity. The present study has been conducted to purify intracellular fat body lipase for the first time, from the last larval instar of Galleria mellonella. Results Purification methods by combination of ammonium sulfate [(NH4)2SO4] precipitation and gel filtration using Sephadex G-100 demonstrated that the amount of protein and the specific activity of fat body lipase were 0.008633 ± 0.000551 mg/ml and 1.5754 ± 0.1042 μmol/min/mg protein, respectively, with a 98.9 fold purity and recovery of 50.81%. Hence, the sephadex G-100 step was more effective in the purification process. SDS-PAGE and zymogram revealed that fat body lipase showed two monomers with molecular weights of 178.8 and 62.6 kDa. Furthermore, biochemical characterization of fat body lipase was carried out through testing its activities against several factors, such as different temperatures, pH ranges, metal ions, and inhibitors ending by determination of their kinetic parameters with the use of p-nitrophenyl butyrate (PNPB) as a substrate. The highest activities of enzyme were determined at the temperature ranges of 35–37 °C and 37–40 °C and pH ranges of 7–9 and 7–10. The partially purified enzyme showed significant stimulation by Ca2+, K+, and Na+ metal ions indicating that fat body lipase is metalloproteinase. Lipase activity was strongly inhibited by some inhibitors; phenylmethylsulfonyl fluoride (PMSF), ethylene-diaminetetractic acid (EDTA), and ethylene glycoltetraacetic acid (EGTA) providing evidence of the presence of serine residue and activation of enzymes by metal ions. Kinetic parameters were 0.316 Umg− 1 Vmax and 301.95 mM Km. Conclusion Considering the purification of fat body lipase from larvae and the usage of some inhibitors especially ion chelating agents, it is suggested to develop a successful control of Galleria mellonella in near future by using lipase inhibitors.

scholarly journals Chemistry and insecticidal potential of bay leaf essential oil against stored grain pest of wheat

2016 ◽  
Vol 8 (4) ◽  
pp. 2049-2054 ◽  
Author(s):  
K. K. Chahal ◽  
Ritima Bansal ◽  
Ramandeep Kaur
Keyword(s):  
Essential Oil ◽  
Column Chromatography ◽  
Insecticidal Activity ◽  
Polar Fraction ◽  
Spectroscopic Techniques ◽  
Stored Grain ◽  
Maximum Inhibition ◽  
Leaf Oil ◽  
Leaf Essential Oil ◽  
Stored Grain Pest

A laboratory experiment was conducted to study chemistry and insecticidal activity of bay leaf oil, its fractions and isolated compounds against stored grain pest of wheat i.e.Tribolium castaneum Herbst. Bay leaf essential oil extracted from dried and powdered bay leaves was subjected to column chromatography to have its fractions. Extensive column chromatography of polar fraction yielded Eugenol and 7, 7 Dimethyl-3-methylene bicyclo [2.2.1] heptan-4-ol which were identified by spectroscopic techniques. Bay leaf oil was tested for its insecticidal activity at five different concentrations in the range 4-12 mg g-1respectively against F1 generation of red rust flour beetle adults. Maximum inhibition was observed at 12 mg g-1concentration. The activity was both time and concentration dependent. The fractions of bay leaf essential oil and the compounds isolated were tested at 4mg g-1 concentration. Polar fraction was found to be more active as compared to nonpolar fraction as 100 and 53.1% mortality was obtained on 30th day for polar and nonpolar fractions, respectively. Comparison of eugenol and 7, 7 Dimethyl-3-methylene bicyclo [2.2.1] heptan-4-ol showed complete mortality on 33rd and 35thday respectively , which revealed that adults of T. castaneum were more susceptible to eugenol.The results indicated that bay leaf essential oil may have potential to control stored grain pest, T. castaneum.

Evaluation of the Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for Control of Sitophilus oryzae

2014 ◽  
Vol 77 (1) ◽  
pp. 87-93 ◽  
Author(s):  
NICKOLAS G. KAVALLIERATOS ◽  
CHRISTOS G. ATHANASSIOU ◽  
MARIA M. AOUNTALA ◽  
DEMETRIUS C. KONTODIMAS
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Sitophilus Oryzae ◽  
Exposure Period ◽  
Isaria Fumosorosea ◽  
Stored Grain ◽  
Stored Grain Pest ◽  
Petri Dishes

The entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea were tested against the stored-grain pest Sitophilus oryzae. The fungi were isolated from the soil (from three locations in Attica, Greece: B. bassiana from Tatoion, M. anisopliae from Marathon, and I. fumosorosea from Aghios Stefanos) using larvae of Galleria mellonella as bait. Suspensions of 2.11 × 107 and 2.11 × 108, 1.77 × 107 and 1.77 × 108, and 1.81 × 107 and 1.81 × 108 conidia per ml of B. bassiana, M. anisopliae, and I. fumosorosea, respectively, were applied by three treatments: (i) sprayed on food and set in petri dishes with adults of S. oryzae, (ii) sprayed on adults of S. oryzae and set in petri dishes without food, and (iii) sprayed on adults of S. oryzae and set in petri dishes with food. The observed mortality of S. oryzae adults during the overall exposure period for the lowest, as well as for the highest, concentrations of B. bassiana, M. anisopliae, and I. fumosorosea ranged from 0 to 100%. Concentration was, in most of the cases tested, a critical parameter that determined the “speed of kill” of the exposed insect species for B. bassiana and M. anisopliae. Conversely, concentration was not that critical for I. fumosorosea, and survival was high in some of the combinations tested, even after 14 days of exposure. Both in the highest and the lowest concentrations of fungi, the mortality of S. oryzae adults was higher when the fungi were applied on adults than when they were applied on food. Higher mortality was observed when food was absent than when food was present, in most of the cases tested. The high efficacy levels recorded in the current study indicate that the tested fungi could be effective biocontrol agents against S. oryzae.

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