Gut-specific arylphorin mediates midgut regenerative response against cry-induced damage in Achaea janata

Survey to assess moth diversity was carried out in Sariska Tiger Reserve of Rajasthan in 2019 from June to November. Total 16 species were recorded under 15 genera, belonging to 5 families for the first time from Sariska Tiger Reserve. Of these, 12 species viz. Eudocima materna Linnaeus, 1767; Eudocima phalonia Linnaeus, 1763; Dysgonia stuposa (Fabricius, 1794); Euproctis divisia Walker, 1855; Achaea janata Linnaeus, 1758; Acherontia styx Westwood, 1847; Daphnis nerii Linnaeus, 1758; Hippotion rosetta (Swinhoe, 1892); Agrius convolvuli Linnaeus, 1758; Nephele hespera Fabricius, 1775; Theretra oldenlandiae (Fabricius, 1775); Caligula lindia Moore, 1865 were first time reports from the state. Moth species were collected with the help of a light sheet system operated every night from 9:00 pm to 2:00 am. Ethyl acetate fumes were used to kill collected speciemens and processed as per standard strategies in Lepidopterology. The identification of moths was carried out with the help of identification keys, standard reference keys. Family Erebidae and Sphingidae are represented by six species each, followed by Saturniidae represented by two species, Noctuidae and Crambidae represented by one species each. This study will improve our understanding of Sariska’s biodiversity and be used to develop strategies for the conservation of moth diversity.

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P5389Limited regenerative potential of the neonatal porcine heart after myocardial infarction

European Heart Journal ◽

10.1093/eurheartj/ehz746.0349 ◽

2019 ◽

Vol 40 (Supplement_1) ◽

Author(s):

K Malliaras ◽

E Polychronopoulou ◽

I Poulakida ◽

D Sagris ◽

K Makaritsis

Keyword(s):

Myocardial Infarction ◽

Cell Cycle ◽

Infarct Size ◽

Border Zone ◽

Large Mammals ◽

Infarct Transmurality ◽

Neonatal Pigs ◽

Regenerative Response ◽

Systolic Thickening ◽

Fractional Shortening

Abstract Background Neonatal murine hearts possess -for a brief period after birth- a robust capacity for spontaneous myocardial regeneration following cardiac injury. Whether hearts of neonatal large mammals possess similar regenerative potential is a matter of active investigation. Recently, two studies reported that 1-day-old and 2-day-old neonatal pigs exhibit a significant cardiac regenerative response post-myocardial infarction (MI), characterized by minimal cardiac fibrosis and spontaneous recovery of left ventricular (LV) function; this regenerative capacity is purportedly lost after the first two days of life. Purpose We sought to evaluate the regenerative potential of neonatal porcine hearts after MI. Methods Twenty-one neonatal farm pigs were randomly assigned to undergo MI by permanent ligation of the left anterior descending artery on postnatal day 1 (P1) or postnatal day 3 (P3). Infarcted P1 and P3 pigs were euthanized either at 1 week or at 7 weeks post-MI. Hearts explanted at 1 week post-MI underwent fluorescent immunohistochemistry for Ki67 and alpha-sarcomeric actinin to quantify myocyte cell cycle re-entry. Transthoracic echocardiography was performed at 7 weeks post-MI to quantify fractional shortening and systolic thickening of the anterior (infarcted) LV wall and the posterior (non-infarcted) LV wall. Hearts explanted at 7 weeks post-MI underwent staining with triphenyl-tetrazolium chloride and Masson's Trichrome to quantify infarct size, infarct circumference and infarct transmurality. Results Fourteen animals successfully completed the protocol. Infarct size (P1: 9.5±2.2% vs P3: 8.9±3.6% of LV, p=0.797), infarct circumference (P1: 33.8±7.1% vs P3: 29.8±10.6% of LV, p=0.566) and infarct transmurality (P1: 38.1±4.3% vs P3: 40.4±13.7% of anterior wall, p=0.764) were comparable in P1 and P3 animals at 7 weeks post-MI. LV fractional shortening (an index of global LV systolic function) was similar in P1 and P3 animals at 7 weeks post-MI (P1: 25.5±2.9% vs P3: 23.7±4.5%, p=0.662). Furthermore, systolic thickening in the anterior (infarcted) LV wall was depressed to a similar degree in P1 and P3 animals (P1: 31.8±5.3% vs P3: 32.3±8.5%, p=0.914) compared to systolic thickening in the posterior (non-infarcted) wall (P1: 72.5±9.0% vs P3: 69.0±11.4%, p=0.666) at 7 weeks post-MI. Myocyte cell cycle re-entry in the infarct border zone was increased in P1 animals compared to P3 animals (P1: 4.5±1.3 vs P3: 2.3±0.6 per field of view, p=0.045) at 1 week post-MI. Conclusions In contrast to recently-published reports, we did not observe a robust cardiac regenerative response in neonatal porcine hearts post-MI. Hearts of both 1-day-old and 3-day-old neonatal pigs exhibited substantial scarring and significant hypokinesia of the infarcted myocardium post-MI. Additional research is warranted to assess the cardiac regenerative potential of neonatal large mammals. Acknowledgement/Funding Co-financed by Greece and the European Union - European Social Fund

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