Influence of cadmium concentration and length of exposure on metabolic rate and gill Na+/K+ ATPase activity of golden shiners (Notemigonus crysoleucas)

2012 ◽  
Vol 156 (1) ◽  
pp. 24-28 ◽  
Author(s):  
John D. Peles ◽  
David H. Pistole ◽  
Mickey Moffe
Keyword(s):  
Atpase Activity ◽  
Metabolic Rate ◽  
Cadmium Concentration ◽  
Notemigonus Crysoleucas ◽  
Golden Shiners

scholarly journals Body mass-specific Na+-K+-ATPase activity in the medullary thick ascending limb: implications for species-dependent urine concentrating mechanisms

2018 ◽  
Vol 314 (4) ◽  
pp. R563-R573 ◽  
Author(s):  
Mun Aw ◽  
Tamara M. Armstrong ◽  
C. Michele Nawata ◽  
Sarah N. Bodine ◽  
Jeeeun J. Oh ◽  
...  
Keyword(s):  
Protein Expression ◽  
Atpase Activity ◽  
Metabolic Rate ◽  
Body Mass ◽  
Whole Body ◽  
Osmotic Gradient ◽  
Thick Ascending Limb ◽  
Rat Strains ◽  
Kangaroo Rat ◽  
Gene And Protein Expression

In general, the mammalian whole body mass-specific metabolic rate correlates positively with maximal urine concentration (Umax) irrespective of whether or not the species have adapted to arid or mesic habitat. Accordingly, we hypothesized that the thick ascending limb (TAL) of a rodent with markedly higher whole body mass-specific metabolism than rat exhibits a substantially higher TAL metabolic rate as estimated by Na+-K+-ATPase activity and Na+-K+-ATPase α1-gene and protein expression. The kangaroo rat inner stripe of the outer medulla exhibits significantly higher mean Na+-K+-ATPase activity (~70%) compared with two rat strains (Sprague-Dawley and Munich-Wistar), extending prior studies showing rat activity exceeds rabbit. Furthermore, higher expression of Na+-K+-ATPase α1-protein (~4- to 6-fold) and mRNA (~13-fold) and higher TAL mitochondrial volume density (~20%) occur in the kangaroo rat compared with both rat strains. Rat TAL Na+-K+-ATPase α1-protein expression is relatively unaffected by body hydration status or, shown previously, by dietary Na+, arguing against confounding effects from two unavoidably dissimilar diets: grain-based diet without water (kangaroo rat) or grain-based diet with water (rat). We conclude that higher TAL Na+-K+-ATPase activity contributes to relationships between whole body mass-specific metabolic rate and high Umax. More vigorous TAL Na+-K+-ATPase activity in kangaroo rat than rat may contribute to its steeper Na+ and urea axial concentration gradients, adding support to a revised model of the urine concentrating mechanism, which hypothesizes a leading role for vigorous active transport of NaCl, rather than countercurrent multiplication, in generating the outer medullary axial osmotic gradient.

scholarly journals Piperine’s mitigation of obesity and diabetes can be explained by its up-regulation of the metabolic rate of resting muscle

2016 ◽  
Vol 113 (46) ◽  
pp. 13009-13014 ◽  
Author(s):  
Leonardo Nogara ◽  
Nariman Naber ◽  
Edward Pate ◽  
Marcella Canton ◽  
Carlo Reggiani ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Skeletal Muscle ◽  
Atpase Activity ◽  
Metabolic Rate ◽  
Muscle Metabolism ◽  
Fluorescent Intensity ◽  
Positive Effects ◽  
Obesity And Diabetes ◽  
Resting Muscle

We identify a target for treating obesity and type 2 diabetes, the consumption of calories by an increase in the metabolic rate of resting skeletal muscle. The metabolic rate of skeletal muscle can be increased by shifting myosin heads from the super-relaxed state (SRX), with a low ATPase activity, to a disordered relaxed state (DRX), with a higher ATPase activity. The shift of myosin heads was detected by a change in fluorescent intensity of a probe attached to the myosin regulatory light chain in skinned skeletal fibers, allowing us to perform a high-throughput screen of 2,128 compounds. The screen identified one compound, which destabilized the super-relaxed state, piperine (the main alkaloid component of black pepper). Destabilization of the SRX by piperine was confirmed by single-nucleotide turnover measurements. The effect was only observed in fast twitch skeletal fibers and not in slow twitch fibers or cardiac tissues. Piperine increased ATPase activity of skinned relaxed fibers by 66 ± 15%. The Kd was ∼2 µM. Piperine had little effect on the mechanics of either fully active or resting muscle fibers. Previous work has shown that piperine can mitigate both obesity and type 2 diabetes in rodent models of these conditions. We propose that the increase in resting muscle metabolism contributes to these positive effects. The results described here show that up-regulation of resting muscle metabolism could treat obesity and type 2 diabetes and that piperine would provide a useful lead compound for the development of these therapies.

scholarly journals Replication of Four Aquatic Reoviruses in Experimentally Infected Golden Shiners (Notemigonus crysoleucas)

1991 ◽  
Vol 27 (3) ◽  
pp. 463-466 ◽  
Author(s):  
Yolanda J. Brady ◽  
John A. Plumb
Keyword(s):  
Notemigonus Crysoleucas ◽  
Golden Shiners

Development of a whole-body cortisol extraction procedure for determination of stress in golden shiners, Notemigonus crysoleucas

2007 ◽  
Vol 33 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Todd David Sink ◽  
Sathyanand Kumaran ◽  
Rebecca T. Lochmann
Keyword(s):  
Extraction Procedure ◽  
Whole Body ◽  
Cortisol Extraction ◽  
Notemigonus Crysoleucas ◽  
Golden Shiners

Some Endrin Relationships in Resistant and Susceptible Populations of Golden Shiners, Notemigonus Crysoleucas

1968 ◽  
Vol 97 (3) ◽  
pp. 260-263 ◽  
Author(s):  
J. Larry Ludke ◽  
Denzel E. Ferguson ◽  
W. David Burke
Keyword(s):  
Notemigonus Crysoleucas ◽  
Golden Shiners ◽  
Susceptible Populations
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