Macroprolactin detection by polyethylene glycol precipitation in a south of Spain health area

2019 ◽  
Vol 493 ◽  
pp. S336
Author(s):  
A. González Raya ◽  
R. Coca Zuñiga ◽  
E. Martín Sálido ◽  
G. Callejón Martín ◽  
A. Léndinez Ramirez ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Health Area ◽  
Polyethylene Glycol Precipitation

scholarly journals Extensive Experience and Validation of Polyethylene Glycol Precipitation as a Screening Method for Macroprolactinemia

1998 ◽  
Vol 44 (8) ◽  
pp. 1758-1759 ◽  
Author(s):  
José Gilberto H Vieira ◽  
Teresinha T Tachibana ◽  
Leda H Obara ◽  
Rui M B Maciel
Keyword(s):  
Polyethylene Glycol ◽  
Screening Method ◽  
Extensive Experience ◽  
Polyethylene Glycol Precipitation

Screening for macroamylase in a community hospital.

1984 ◽  
Vol 30 (5) ◽  
pp. 741-742 ◽  
Author(s):  
C A Isham ◽  
N A Ridgeway ◽  
R Hedrick ◽  
J C Cate
Keyword(s):  
Polyethylene Glycol ◽  
Community Hospital ◽  
Serum Amylase ◽  
Gel Filtration ◽  
Screening Procedure ◽  
Gel Filtration Chromatography ◽  
Polyethylene Glycol Precipitation ◽  
Precipitation Test

Abstract We evaluated the polyethylene glycol precipitation test (Gastroenterology 83: 378-382, 1982), looking for macroamylase in the serum of 66 patients whose values for serum amylase were above normal. Three patients (4.5%) were identified by this method as having macroamylase , and this was confirmed by gel-filtration chromatography and electrophoresis. We find this to be the test of choice as a screening procedure for macroamylasemia because of its speed, simplicity, and apparent reliability. Diagnosis of macroamylasemia is important in preventing needless treatment and investigation for pancreatitis.

Evaluation of percentage recovery together with modified reference range in hyperprolactinemia

2019 ◽  
Vol 45 (1) ◽  
pp. 37-43
Author(s):  
Melahat Dirican ◽  
Hacer Ebru Açıkgöz ◽  
Emre Sarandöl
Keyword(s):  
Polyethylene Glycol ◽  
Reference Range ◽  
Added Value ◽  
Percentage Recovery ◽  
Before And After ◽  
Recovery Percentage ◽  
Discrimination Capacity ◽  
Polyethylene Glycol Precipitation

Abstract Objective Macroprolactinemia is an important cause of hyperprolactinemia. The aim of this study was to examine the added value of the consideration of modified reference range in determination of macroprolactinemia and true hyperprolactinemia. Materials and methods Three hundred and ninety patients with high and 131 with normal prolactin (PRL) levels were included in this study. PRL had been analyzed before and after polyethylene glycol precipitation (post-PEG PRL). Recovery percentage (R%) <40% and >60% had been reported as macroprolactinemia and true hyperprolactinemia, respectively. Post-PEG PRL levels were evaluated according to the modified reference range obtained from those of the normoprolactinemic subjects. Results According to the R% criterion; macroprolactinemia had been detected in 24.9% and true hyperprolactinemia in 67.4% of hyperprolactinemic patients. When the data were evaluated considering the post-PEG PRL levels according to the modified reference range; 13 (13.4%) of the 97 macroprolactinemia reports would be considered as true hyperprolactinemia and 6 (2.3%) of the 263 true hyperprolactinemia reports would be changed as macroprolactinemia. Conclusion Discrimination capacity of R% criterion for true hyperprolactinemia and macroprolactinemia is limited, and we suggest that, in accordance with R% criterion, laboratory reports should include the post-PEG PRL levels along with the modified reference range.

scholarly journals Large-scale purification of hepatitis B surface antigen

1976 ◽  
Vol 3 (6) ◽  
pp. 626-631
Author(s):  
J Vnek ◽  
A M Prince
Keyword(s):  
Polyethylene Glycol ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Phosphate Buffer ◽  
Sodium Phosphate ◽  
Large Scale ◽  
Hepatitis B Surface Antigen ◽  
Sodium Phosphate Buffer ◽  
Subsequent Elution ◽  
Polyethylene Glycol Precipitation

Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02 M sodium phosphate buffer.

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