Evaluation of ldl cholesterol: Martin equation versus direct measurement for triglycerides levels above 400 mg/dl

2019 ◽  
Vol 493 ◽  
pp. S79
Author(s):  
N.M. Sumita ◽  
J. De Sa ◽  
S.J. Mena ◽  
J.S. Jangrossi ◽  
G. Loureiro
Keyword(s):  
Direct Measurement ◽  
Ldl Cholesterol ◽  
Equation Versus

Direct Measurement of Low Density Lipoprotein in Whole Blood by Silver-Enhanced Gold-Labelled Immunoassay

1992 ◽  
Vol 29 (3) ◽  
pp. 283-286 ◽  
Author(s):  
Nishith Patel ◽  
Bernard F Rocks ◽  
S Andrew Iversen
Keyword(s):  
Direct Measurement ◽  
Whole Blood ◽  
Ldl Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Silver Ions ◽  
Low Density ◽  
Good Precision ◽  
Blood Samples ◽  
Antigen Complex

A competitive silver-enhanced gold-labelled immunoassay has been developed for the direct measurement of low density lipoprotein (LDL) in whole blood. Immobilized LDL and sample LDL compete for added antibody. Quantitation of the bound antibody/antigen complex is achieved by the addition of gold-labelled antiimmunoglobulin G followed by enhancement of absorbance by addition of silver ions. Whole-blood samples from fasting patients were assayed directly for LDL by the procedure and the corresponding plasma samples were assayed for total cholesterol, high density lipoprotein and triglycerides followed by the indirect calculation of LDL cholesterol. The correlation between the two methods was good (r = 0·82) and the SEGLISA exhibited good precision.

Comparison of Friedwald formula with direct measurement of LDL-cholesterol

2011 ◽  
Vol 44 (13) ◽  
pp. S147
Author(s):  
Morad Rostami ◽  
Mohammad Aberomand ◽  
Alireza Khirollah ◽  
Masoomeh Jorfi ◽  
Reza Malihi ◽  
...  
Keyword(s):  
Direct Measurement ◽  
Ldl Cholesterol

scholarly journals Direct measurement of LDL cholesterol

1996 ◽  
Vol 42 (5) ◽  
pp. 780-781 ◽  
Author(s):  
S S Levinson ◽  
J J Maciejko
Keyword(s):  
Direct Measurement ◽  
Ldl Cholesterol

scholarly journals LDL-cholesterol: Friedewald calculated versus direct measurement-study from a large Indian laboratory database

2014 ◽  
Vol 18 (4) ◽  
pp. 502 ◽  
Author(s):  
Subramanian Kannan ◽  
Shriraam Mahadevan ◽  
Bharath Ramji ◽  
Muthukumaran Jayapaul ◽  
V Kumaravel
Keyword(s):  
Direct Measurement ◽  
Ldl Cholesterol ◽  
Measurement Study

Comparison of an Immunoprecipitation Method for Direct Measurement of LDL-Cholesterol With Beta-Quantification (Ultracentrifugation)

1995 ◽  
Vol 104 (1) ◽  
pp. 76-81 ◽  
Author(s):  
Ishwarlal Jialal ◽  
Shaina V. Hirany ◽  
Sridevi Devaraj ◽  
Thomas A. Sherwood
Keyword(s):  
Direct Measurement ◽  
Ldl Cholesterol

scholarly journals Reliability of Friedewald formula in patients with type 2 diabetes mellitus and its relation to lipid profile in diabetes regulation

2021 ◽  
Vol 29 (2) ◽  
pp. 179-187
Author(s):  
Didem Barlak Keti ◽  
Sabahattin Muhtaroglu
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Profile ◽  
Direct Measurement ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Cholesterol Ratio ◽  
Cholesterol Levels ◽  
Type 2 Dm ◽  
Friedewald Formula

Abstract Introduction: Many laboratories utilize Friedewald formula (FF) to analyze LDL cholesterol levels of patients including diabetes mellitus (DM). Therefore, it is essential to consider the coherence of results acquired by FF and direct measurement. The number of studies that investigated the effect of lipid parameters, especially TG/HDL cholesterol ratio, on the difference between the two methods is limited. The study was designed to compare LDL cholesterol values obtained by using FF with direct measurement, and to evaluate the relationship between diabetes regulation and lipid profile. Material and Methods: In the cross-sectional study, 529 type 2 DM patients and 1703 non-DM subjects were divided into four groups regarding TG concentrations. Unlike other studies, the study focuses on direct LDL (DLDL) cholesterol levels obtained with the help of different DLDL cholesterol kits (n=20). The correlations were implemented between HbA1c and lipid profiles. Results: It was determined that the bias% was over 10% in 24% of patients with 100-199 mg/dL TG levels. The parameter revealed that the most significant difference and the strongest correlation with HbA1c was TG/HDL cholesterol ratio in patients with type 2 DM. Conclusions: In patients with type 2 DM, even if it was TG <200 mg/dL, LDL calculated with FF should be evaluated together with the TG/HDL cholesterol ratio. Otherwise, direct measurement can be recommended. This ratio is related to diabetes regulation and may be used to monitor patients..

scholarly journals Evaluation of Recently Developed Regression Equation with Direct Measurement of Low-density Lipoprotein Cholesterol in a Bangladeshi Population

2015 ◽  
Vol 5 (2) ◽  
pp. 75-79
Author(s):  
Md Fazley Rabbi Sha ◽  
Abdul Hai Siddique ◽  
Muhammad Saiedullah ◽  
Md Aminul Haque Khan
Keyword(s):  
Direct Measurement ◽  
Ldl Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Correlation Coefficients ◽  
Mean Value ◽  
Lipid Profiles ◽  
Regression Equation ◽  
Low Density ◽  
Significant Difference

Background: Meaningful underestimation of low-density lipoprotein (LDL) cholesterol is an important shortcoming of Friedewald’s formula (FF) at higher triglyceride (TG) levels. Recently a regression equation (RE) has been developed using lipid profiles in one setting and validated externally for the calculation of LDL cholesterol. This newly developed RE requires more studies in different settings.Objective: The aim of this study was to evaluate the performance of the regression equation against direct measurement.Materials and Methods: Lipid profiles of 600 subjects attending a tertiary healthcare center were included in this study. Specimens were collected and lipid profiles were measured by standard methods. Sixty two lipid profiles with TG above 400 mg/dL were excluded. Calculated LDL cholesterol values using FF and RE were compared with measured LDL cholesterol by Pearson’s correlation test, Passing & Bablok regression, accuracy within ±5% and ±12% of measured LDL cholesterol and two-tailed paired t test at various TG ranges.Results: The mean value of LDL cholesterol was 148.6 ± 37.2 mg/dL for direct measurement, 146.9 ± 42.4 mg/dL for FF and 148.6 ± 34.7 mg/dL for RE. The correlation coefficients of calculated LDL cholesterol values with measured LDL cholesterol were 0.949 (p<0.001) for FF and 0.959 (p<0.001) for RE. Passing & Bablok regression equation against measured LDL cholesterol was y = 0.897x + 16.2 for FF and y = 1.0842x – 13.1 for RE. Accuracy within ±5% of measured LDL cholesterol was 45% for FF, 57% for RE and within ±12% of measured LDL cholesterol was 84% for FF, 93% for RE. When calculated LDL cholesterol was compared with measured LDL cholesterol at different TG ranges, FF significantly underestimated LDL cholesterol at TG concentrations above 200 mg/dL whereas no significant difference was observed for RE.Conclusion: This study reveals that RE equation has similar performance to direct measurement for calculation of LDL cholesterolJ Enam Med Col 2015; 5(2): 75-79

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