Rlim, an E3 ubiquitin ligase, influences the stability of Stathmin protein in human osteosarcoma cells

Background. Tripartite motif containing 58 (TRIM58), an E3 ubiquitin ligase, is reported as a suppressor gene in certain human tumors. However, the biological function of TRIM58 in osteosarcoma (OS) is still less identified. Methods. In the present study, TRIM58 induced silencing and overexpression in OS cells using RNA interference (RNAi) and lentiviral-mediated vector, respectively. Cell proliferation profiles were analyzed using cell counting kit-8 (CCK-8) assay. Cell apoptosis profiles were determined using a flow cytometer. qRT-PCR and western blot were used to determine gene expression. Coimmunoprecipitation (Co-IP) assay was used to examine protein interaction. Results. Our results demonstrated TRIM58 was downregulated in human OS tissues. Overexpression of TRIM58 remarkably suppressed the growth of OS cells and decreased glucose transportation and lactate secretion. These results indicated that TRIM58 involved in the regulation of energy metabolism in OS cells. Importantly, TRIM58 interacted with pyruvate kinase M2 (PKM2) in OS cells. Moreover, TRIM58 might inhibit the activity of PKM2 through enhancing its polyubiquitination in OS cells. Conclusions. This analysis not only explored a deep understanding of the biological function of TRIM58 but also indicated its signaling pathway in OS cells.

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Transfection and overexpression of 11-ß-hydroxysteroid kinase type 1 in human osteosarcoma cells (HOS 58) and primary human osteoblasts

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-2006-932987 ◽

2006 ◽

Vol 114 (S 1) ◽

Author(s):

PJ Braun ◽

V Ritz ◽

V Bähr ◽

S Diederich ◽

M Hüfner ◽

...

Keyword(s):

Osteosarcoma Cells ◽

Human Osteoblasts ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells ◽

Primary Human Osteoblasts

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Antiproliferative properties of oleuropein in human osteosarcoma cells

Bone Abstracts ◽

10.1530/boneabs.5.p109 ◽

2016 ◽

Author(s):

Jose M Moran ◽

Olga Leal-Hernandez ◽

Maria L Canal-Macias ◽

Jesus M Lavado-Garcia ◽

Raul Roncero-Martin ◽

...

Keyword(s):

Osteosarcoma Cells ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells

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Faculty Opinions recommendation of Ubiquitin-specific protease 19 regulates the stability of the E3 ubiquitin ligase MARCH6.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718519896.793514105 ◽

2016 ◽

Author(s):

Simon Wing

Keyword(s):

Ubiquitin Ligase ◽

E3 Ubiquitin Ligase ◽

Specific Protease ◽

Ubiquitin Specific Protease ◽

The Stability

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Copper(II) Complexes with Saccharinate and Glutamine as Antitumor Agents: Cytoand Genotoxicity in Human Osteosarcoma Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520616666160513130204 ◽

2017 ◽

Vol 17 (3) ◽

pp. 424-433 ◽

Cited By ~ 3

Author(s):

Juan Cadavid-Vargas ◽

Ignacio Leon ◽

Susana Etcheverry ◽

Eduardo Santi ◽

Maria Torre ◽

...

Keyword(s):

Antitumor Agents ◽

Osteosarcoma Cells ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells

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Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells

Nutrients ◽

10.3390/nu13020354 ◽

2021 ◽

Vol 13 (2) ◽

pp. 354

Author(s):

Katerina Gioti ◽

Anastasia Papachristodoulou ◽

Dimitra Benaki ◽

Nektarios Aligiannis ◽

Alexios-Leandros Skaltsounis ◽

...

Keyword(s):

Chemotherapeutic Agent ◽

Molecular Techniques ◽

Osteosarcoma Cells ◽

Elisa Method ◽

Chain Reaction ◽

Cytotoxic Effects ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells ◽

Anti Cancer ◽

Polymerase Chain

Oleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of Olea europaea L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it presents significant side effects. The aim of the present study was to investigate the effect of oleuropein alone (20 μg/mL) and in co-treatment with ADR (50 nM), in MG-63 human osteosarcoma cells. Therefore, cellular and molecular techniques, such as MTT assay, flow cytometry, real-time Polymerase Chain Reaction (PCR), western blot and Elisa method, as well as Nuclear Magnetic Resonance (NMR) spectroscopy, were applied to unveil changes in the signal transduction pathways involved in osteosarcoma cells survival. The observed alterations in gene, protein and metabolite levels denote that OLEU not only inhibits MG-63 cells proliferation and potentiates ADR’s cytotoxicity, but also exerts its action, at least in part, through the induction of autophagy.

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