A Dynamic Folded Hairpin Conformation Is Associated with α-Globin Activation in Erythroid Cells

Abstract Erythrocyte protein 4.2 (P4.2) is an important component of the erythrocyte membrane skeletal network with an undefined biologic function. Presently, very little is known about the expression of the P4.2 gene during mouse embryonic development and in adult animals. By using the Northern blot and in situ hybridization techniques, we have examined the spatial and temporal expression of the P4.2 gene during mouse development. We show that expression of the mouse P4.2 gene is temporally regulated during embryogenesis and that the P4.2 mRNA expression pattern coincides with the timing of erythropoietic activity in hematopoietic organs. P4.2 transcripts are first detected in embryos on day 7.5 of gestation and are localized exclusively in primitive erythroid cells of yolk sac origin. These erythroid cells remain to be the only source for P4.2 expression until the switch of the hematopoietic producing site to fetal liver. In mid- and late-gestation periods, P4.2 mRNA expression is restricted to the erythroid cells in fetal liver and to circulating erythrocytes. Around and after birth, the site for P4.2 expression is switched from liver to spleen and bone marrow, and P4.2 transcripts are only detected in cells of the erythroid lineage. These results provide the evidence for specific P4.2 expression in erythroid cells. In addition, the timing and pattern of expression of the P4.2 gene suggest the specific regulation of the P4.2 gene.

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Tissue-Specific Regulation of Iron Metabolism and Heme Synthesis: Distinct Control Mechanisms in Erythroid Cells

Blood ◽

10.1182/blood.v89.1.1.1_1_25 ◽

1997 ◽

Vol 89 (1) ◽

pp. 1-25 ◽

Cited By ~ 8

Author(s):

Prem Ponka

Keyword(s):

Iron Metabolism ◽

Control Mechanisms ◽

Erythroid Cells ◽

Tissue Specific ◽

Heme Synthesis ◽

Specific Regulation ◽

In Erythroid Cells

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Faculty Opinions recommendation of Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1029492.345433 ◽

2005 ◽

Author(s):

Caroline Philpott

Keyword(s):

Iron Uptake ◽

Erythroid Cells ◽

In Erythroid Cells

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Palmitoylation of MPP1 (membrane-palmitoylated protein 1)/p55 is crucial for lateral membrane organization in erythroid cells.

Journal of Biological Chemistry ◽

10.1074/jbc.w118.002209 ◽

2018 ◽

Vol 293 (8) ◽

pp. 2786-2786

Author(s):

Agnieszka Łach ◽

Michal Grzybek ◽

Elżbieta Heger ◽

Justyna Korycka ◽

Marcin Wolny ◽

...

Keyword(s):

Erythroid Cells ◽

Membrane Organization ◽

Lateral Membrane ◽

In Erythroid Cells

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trans-Activation of a globin promoter in nonerythroid cells

Molecular and Cellular Biology ◽

10.1128/mcb.11.2.843-853.1991 ◽

1991 ◽

Vol 11 (2) ◽

pp. 843-853

Author(s):

T Evans ◽

G Felsenfeld

Keyword(s):

Dna Binding ◽

Binding Sites ◽

Gene Activation ◽

Specific Factor ◽

Erythroid Cells ◽

Specific Manner ◽

Alpha Globin ◽

A Cell ◽

Globin Promoter ◽

In Erythroid Cells

We show that expression in fibroblasts of a single cDNA, encoding the erythroid DNA-binding protein Eryf1 (GF-1, NF-E1), very efficiently activates transcription of a chicken alpha-globin promoter, trans-Activation in these cells occurred when Eryf1 bound to a single site within a minimal globin promoter. In contrast, efficient activation in erythroid cells required multiple Eryf1 binding sites. Our results indicate that mechanisms exist that are capable of modulating the trans-acting capabilities of Eryf1 in a cell-specific manner, without affecting DNA binding. The response of the minimal globin promoter to Eryf1 in fibroblasts was at least as great as for optimal constructions in erythroid cells. Therefore, the assay provides a very simple and sensitive system with which to study gene activation by a tissue-specific factor.

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