Effect of Concentration and Substrate Flow Rate on Isomaltulose Production from Sucrose by Erwinia sp. Cells Immobilized in Calcium-Alginate Using Packed Bed Reactor

2010 ◽  
Vol 162 (1) ◽  
pp. 89-102 ◽  
Author(s):  
Haroldo Yukio Kawaguti ◽  
Hélia Harumi Sato
Keyword(s):  
Flow Rate ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Packed Bed Reactor

scholarly journals Continuous Production of Lipase-Catalyzed Biodiesel in a Packed-Bed Reactor: Optimization and Enzyme Reuse Study

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Hsiao-Ching Chen ◽  
Hen-Yi Ju ◽  
Tsung-Ta Wu ◽  
Yung-Chuan Liu ◽  
Chih-Chen Lee ◽  
...  
Keyword(s):  
Flow Rate ◽  
Immobilized Lipase ◽  
Continuous Production ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Molar Ratio ◽  
System Response ◽  
Biodiesel Synthesis ◽  
Substrate Molar Ratio ◽  
Molar Conversion

An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in atert-butanol solvent system. Response surface methodology (RSM) and Box-Behnken design were employed to evaluate the effects of reaction temperature, flow rate, and substrate molar ratio on the molar conversion of biodiesel. The results showed that flow rate and temperature have significant effects on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions were as follows: flow rate 0.1 mL/min, temperature52.1∘C, and substrate molar ratio 1 : 4. The predicted and experimental values of molar conversion were83.31±2.07% and82.81±.98%, respectively. Furthermore, the continuous process over 30 days showed no appreciable decrease in the molar conversion. The paper demonstrates the applicability of using immobilized lipase and a packed-bed reactor for continuous biodiesel synthesis.

scholarly journals Development of a bioreactor system for the remediation of endosulphan

2018 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
K. Jesitha ◽  
P. S. Harikumar
Keyword(s):  
Detection Limit ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Alginate Beads ◽  
Soil Samples ◽  
Packed Bed Reactor ◽  
Contaminated Water ◽  
Initial Concentration ◽  
Bioreactor System ◽  
Ca Alginate

Abstract A bioreactor system that consisted of Pseudomonas fluorescens cells immobilised in calcium-alginate beads was utilised to remediate endosulphan contaminated water and soil. A packed bed reactor system was designed for the bio-degradation of endosulphan in artificially spiked water samples (initial concentration of endosulphan: 350 µg/L). Reactor studies with cell-immobilised Ca-alginate beads were conducted after checking their efficiency through batch and column degradation studies. The results showed that the concentration of toxic isomers of endosulphan (endosulphan alpha and endosulphan beta) was below the limit in the bioreactor during the 7th day of the experiment. Experiments conducted with contaminated soil samples (initial concentration of endosulphan: 1,000 μg/kg) indicated that the toxic isomers of endosulphan degraded to below the detection limit within 10 days and monitoring of endosulphan residues on the 14th day revealed that almost complete degradation of metabolites of endosulphan had occurred. The bioreactor system designed can be scaled up for remediation of endosulphan in contaminated areas.

scholarly journals WALL EFFECT OF A PACKED BED WITH PELLET PARTICLES

2018 ◽  
Vol 56 (2A) ◽  
pp. 31-36
Author(s):  
Tran Duy Hai
Keyword(s):  
Flow Rate ◽  
Particle Diameter ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Radial Coordinate ◽  
Flow Profile ◽  
Local Velocity ◽  
Gas Velocity ◽  
Bed Height ◽  
Small Ratio

Fluid flow profile is a dominate role in the performance of packed bed reactor. In small ratio of column-to-particle diameter, velocity pattern is strongly affected by voidage distribution, which depends on radial coordinate, flow rate and bed height. In this study, effects of voidage distribution to gas velocity profile in a packed bed with pellet particles was empirically investigated. Uniformity of local velocity at the top of the bed was clearly observed with decreasing of bed height and flow rate. For 400 mm of bed height, the measured velocities are a well fitting to Fahien and Stankovich model for any expected flow rate.

High renin productivity of rCHO cells cultivated in radial-flow nonwoven fabric mat packed-bed reactor with increasing circulating flow rate

1997 ◽  
Vol 83 (5) ◽  
pp. 443-450 ◽  
Author(s):  
Maki Motobu ◽  
Shigeru Matsuo ◽  
Pi-Chao Wang ◽  
Hiroshi Kataoka ◽  
Masatoshi Matsumura
Keyword(s):  
Flow Rate ◽  
Radial Flow ◽  
Packed Bed ◽  
Nonwoven Fabric ◽  
Packed Bed Reactor

scholarly journals Continuous Production of 2-Phenylethyl Acetate in a Solvent-Free System Using a Packed-Bed Reactor with Novozym® 435

Catalysts ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 714 ◽  
Author(s):  
Shang-Ming Huang ◽  
Hsin-Yi Huang ◽  
Yu-Min Chen ◽  
Chia-Hung Kuo ◽  
Chwen-Jen Shieh
Keyword(s):  
Chemical Synthesis ◽  
Flow Rate ◽  
Packed Bed ◽  
Solvent Free ◽  
Packed Bed Reactor ◽  
Packed Bed Bioreactor ◽  
Free System ◽  
Phenethyl Alcohol ◽  
Solvent Free System ◽  
Molar Conversion

2-Phenylethyl acetate (2-PEAc), a highly valued natural volatile ester, with a rose-like odor, is widely added in cosmetics, soaps, foods, and drinks to strengthen scent or flavour. Nowadays, 2-PEAc are commonly produced by chemical synthesis or extraction. Alternatively, biocatalysis is a potential method to replace chemical synthesis or extraction for the production of natural flavour. Continuous synthesis of 2-PEAc in a solvent-free system using a packed bed bioreactor through immobilized lipase-catalyzed transesterification of ethyl acetate (EA) with 2-phenethyl alcohol was studied. A Box–Behnken experimental design with three-level-three-factor, including 2-phenethyl alcohol (2-PE) concentration (100–500 mM), flow rate (1–5 mL min−1) and reaction temperature (45–65 °C), was selected to investigate their influence on the molar conversion of 2-PEAc. Then, response surface methodology and ridge max analysis were used to discuss in detail the optimal reaction conditions for the synthesis of 2-PEAc. The results indicated both 2-PE concentration and flow rate are significant factors in the molar conversion of 2-PEAc. Based on the ridge max analysis, the maximum molar conversion was 99.01 ± 0.09% under optimal conditions at a 2-PE concentration of 62.07 mM, a flow rate of 2.75 mL min−1, and a temperature of 54.03 °C, respectively. The continuous packed bed bioreactor showed good stability for 2-PEAc production, enabling operation for at least 72 h without a significant decrease of conversion.

scholarly journals Computational fluid dynamics analysis of flow through immobilized catalyzed packed bed reactor for removal of 4-chlorophenol from wastewater

2019 ◽  
Vol 25 (6) ◽  
pp. 878-889 ◽  
Author(s):  
Sudhansu Sandhibigraha ◽  
Soumya Sasmal ◽  
Tarun Kanti Bandyopadhyay ◽  
Biswanath Bhunia
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Flow Rate ◽  
Cfd Simulation ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Inlet Concentration ◽  
Bed Height ◽  
Fluent Software ◽  
Flow Through

The computational fluid dynamics (CFD) simulation of the packed bed reactor (PBR) was carried out using ANSYS Fluent software. The various process parameters, such as inlet concentration of 4-chlorophenol (4-CP), flow rate, bed height, and porosity, were optimized to predict maximum biodegradation of 4-CP in immobilized catalyzed PBR. The geometrical mesh of the PBR was constructed using Gambit software, and a mesh size of 236995 was selected from the grid-independent study. A laminar flow model was used to understand the hydrodynamics as well as concentration profile of 4-CP inside the PBR using Fluent software. Through CFD, the effect of the flow rate, inlet concentration, and the bed height and porosity of the immobilized catalyst bed on the static pressure, mass imbalance, velocity, and stress-strain field inside the PBR was visualized. CFD simulation study predicted that maximum biodegradation of 4-CP was found in the presence of 500 mg/L of inlet concentration of 4-CP, 4 mL/min of flow rate, 18 cm of bed height and 0.375 of porosity. An experimental study was conducted for wastewater flow through the <i>B. subtilis MF447840.1</i> immobilized catalyzed PBR to remove the 4-CP in the laminar flow region. It was evident that CFD simulated results agreed well with experimental values.

Mass transfer studies on the reduction of Cr(VI) using calcium alginate immobilized Bacillus sp. in packed bed reactor

2010 ◽  
Vol 101 (3) ◽  
pp. 853-858 ◽  
Author(s):  
Mathur Nadarajan Kathiravan ◽  
Ravikumar Karthiga Rani ◽  
Ramalingam Karthick ◽  
Karuppan Muthukumar
Keyword(s):  
Mass Transfer ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Bacillus Sp ◽  
Mass Transfer Studies
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