Toxicity of TiO2 nanoparticle to denitrifying strain CFY1 and the impact on microbial community structures in activated sludge

Chemosphere ◽  
2016 ◽  
Vol 144 ◽  
pp. 1334-1341 ◽  
Author(s):  
Dapeng Li ◽  
Bin Li ◽  
Qiaoruo Wang ◽  
Ning Hou ◽  
Chunyan Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Tio2 Nanoparticle ◽  
Community Structures ◽  
Microbial Community Structures ◽  
The Impact

Effects of Hg2+ on Microbial Community Structures of Aerobic Activated Sludge

2011 ◽  
Vol 343-344 ◽  
pp. 333-339
Author(s):  
Ning Dai ◽  
Jin Sheng Wang ◽  
Yan Guo Teng ◽  
Jie Su
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Nested Pcr ◽  
Operation Time ◽  
Community Structures ◽  
Microbial Species ◽  
Obvious Change ◽  
Microbial Community Structures ◽  
Operation Period

The effects of Hg2+ on microbial community structures of aerobic activated sludge were investigated by nested PCR-DGGE techniques. The results showed that the microbial community of each group might have obvious change compared with the group without Hg2+ in the inflow, especially when the Hg2+ concentration was 46.87mg/L in the inflow, and the microbial community changed obviously with the extend of operation time. The variance of microbial species become obvious in each activated sludge group with the extend of operation time. Compared with the group without Hg2+ in the inflow, the dominant microbial species of each activated sludge group changed, and the dominant microbial species of the group whose concentration of Hg2+ increased gradually in the inflow had the biggest changes after 60 days. Hg2+ in the inflow may stimulate the growth of Actinomycetes after an operation period of 30 days.

Linking nitrification characteristic and microbial community structures in integrated fixed film activated sludge reactor by high-throughput sequencing

2016 ◽  
Vol 74 (6) ◽  
pp. 1354-1364 ◽  
Author(s):  
Bin Dong ◽  
Jie Tan ◽  
Yang Yang ◽  
Zishan Pang ◽  
Zhongtian Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Ammonia Removal ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Nitrifying Bacteria ◽  
Ammonia Oxidizing Bacteria ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Fixed Film

The primary goal of this study is to investigate ammonia removal, abundance of nitrifying bacteria and microbial community structures in a laboratory-scale integrated fixed film activated sludge (IFAS) reactor. The results of Illumina MiSeq sequencing based on 16S rRNA genes showed Proteobacteria and Bacteroidetes were the dominant phyla in both biofilm and suspended sludge samples in the IFAS reactor. The dominant ammonia-oxidizing bacteria (AOB) species was Nitrosomonas and the dominant nitrite-oxidizing bacteria species was Nitrospira. The contribution of biofilm to ammonia removal increased from 4.0 ± 0.9% to 37.0 ± 2% when the temperature decreased from 25 °C to 10 °C. The real-time polymerase chain reaction (PCR) result showed the abundance of AOB in suspended sludge was higher than that in biofilm at the same time. However, nitrification is more dependent on attached growth than on suspended growth in the IFAS reactor at 15 °C and 10 °C and the abundance of AOB in biofilm was also higher than that in suspended sludge. The more robust ammonia removal rate at low temperatures by biofilm contributed to the relatively stable ammonia removal, and biofilm attached on carriers in the IFAS reactor is advantageous for nitrification in low-temperature environment.

Application of molecular methods to microbial community analysis of activated sludge

2000 ◽  
Vol 42 (3-4) ◽  
pp. 17-22 ◽  
Author(s):  
M. Onuki ◽  
H. Satoh ◽  
T. Mino ◽  
T. Matsuo
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wastewater Treatment Plants ◽  
Community Analysis ◽  
Molecular Methods ◽  
Microbial Community Analysis ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Gradient Gel Electrophoresis

In the last decade, molecular biology has made significant progress, and innovative molecular methods have become available to analyze microbial community structures. Among them, we applied the FISH (Fluorescent in situ Hybridization) method to analyze activated sludge in wastewater treatment plants (WWTPs). As a result, domain- or division-level community structures in activated sludge were determined successfully without cultivation. We also applied the PCR (polymerase chain reaction) -DGGE (Denaturing Gradient Gel Electrophoresis) method for laboratory nitrifying sludge in order to investigate more detailed microbial community structure. By this method, genus- or species-level community structures were characterized well. This method was also found to be powerful for monitoring the change of microbial community structures. For example, the behavior of Nitrosomonas group was successfully detected in the reactor with nitrification by the PCR-DGGE method.

Revealing microbial community structures in large- and small-scale activated sludge systems by barcoded pyrosequencing of 16S rRNA gene

2012 ◽  
Vol 66 (10) ◽  
pp. 2155-2161 ◽  
Author(s):  
Purnika Damindi Ranasinghe ◽  
Hiroyasu Satoh ◽  
Mamoru Oshiki ◽  
Kenshiro Oshima ◽  
Wataru Suda ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
16S Rrna ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Small Scale ◽  
Rrna Gene ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Insight Into

The diversity of bacterial groups in activated sludge from large- and small-scale wastewater treatment plants was explored by barcoded pyrosequencing of 16S rRNA gene. Activated sludge samples (three small and 17 large scale) were collected from 12 wastewater treatment plants to clarify precise taxonomy and relative abundances. DNA was extracted, and amplified by 4 base barcoded 27f/519r primer set. The 454 Titanium (Roche) pyrosequences were obtained and analyses performed by Quantitative Insight Into Microbial Ecology (QIIME) with around 100,000 reads. Sequence statistics were computed, while constructing a phylogenetic tree and heatmap. Computed results explained total microbial diversity at phylum and class level and resolution was further extended to Operational Taxonomic Unit (OTU) based taxonomic assignment for investigating community distribution based on individual sample. Composition of sequence reads were compared and microbial community structures for large- and small-scale treatment plants were identified as major phyla (Proteobacteria and Bacteroidetes) and classes (Betaproteobacteria and Bacteroidetes). Also, family level breakdowns were explained and differences in family Nitrospiraceae and phylum Actinobacteria found at their species level were also illustrated. Thus, the pyrosequencing method provides high resolution insight into microbial community structures in activated sludge that might have been unnoticed with conventional approaches.

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