Cadmium biosorption and biomass production by two freshwater microalgae Scenedesmus acutus and Chlorella pyrenoidosa: An integrated approach

The present study investigates the possibility of integrating an existing industrial large scale biomass production with the treatment of waste water in which a mixture of organic and inorganic rich pollutants was used as a medium. This study suggests that the replacement of a defined medium with a complete mixotrophic medium gives a significant statistical difference in terms of growth parameters i.e. biomass production and specific growth rate. The green microalga C. pyrenoidosa was cultivated under different mixotrophic conditions for evaluation of biomass production. Inorganic defined fog’s medium supplemented, with raw dairy wastewater led to 1.37g/L biomass production in comparison to 1.2g/L obtained with pure glucose revealing 14.16% increase. The study also involves the supplementation of raw dairy wastewater as an organic carbon source in an inorganic medium comprising municipal treated water and reverse osmosis (RO) treated wastewater and attained 2.4g/L and 1.6g/L of biomass respectively, as compared to 0.3g/L and 0.16g/L obtained in the wastewaters alone revealing 700% and 900% increase respectively. Mixotrophic regimen cells as analyzed by a 2D Fourier transform infrared (FTIR) spectroscopy for its biochemical content revealed that fog’s blended raw dairy waste (RDW) regimen cells had maximum Carbohydrate/Amide ratio. The study suggests that the mixotrophic regimen C. pyrenoidosa cells can show appropriate growth in a mixture of waste waters and the same comes out to be a cost effective and feasible alternative commercial medium for biomass production without requiring any expensive organic carbon sources in the culture medium.

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Evaluation of autotrophic and mixotrophic regimen Chlorella pyrenoidosa cells in various wastes water for its biochemical composition and biomass production

10.7287/peerj.preprints.681 ◽

2014 ◽

Author(s):

Nisha Phour Dhull ◽

Raman Soni ◽

Deepak Kumar Rahi ◽

Sanjeev Kumar Soni

Keyword(s):

Organic Carbon ◽

Biomass Production ◽

Large Scale ◽

Growth Parameters ◽

Carbon Sources ◽

Cost Effective ◽

Chlorella Pyrenoidosa ◽

Defined Medium ◽

Dairy Wastewater ◽

Treated Wastewater

The present study investigates the possibility of integrating an existing industrial large scale biomass production with the treatment of waste water in which a mixture of organic and inorganic rich pollutants was used as a medium. This study suggests that the replacement of a defined medium with a complete mixotrophic medium gives a significant statistical difference in terms of growth parameters i.e. biomass production and specific growth rate. The green microalga C. pyrenoidosa was cultivated under different mixotrophic conditions for evaluation of biomass production. Inorganic defined fog’s medium supplemented, with raw dairy wastewater led to 1.37g/L biomass production in comparison to 1.2g/L obtained with pure glucose revealing 14.16% increase. The study also involves the supplementation of raw dairy wastewater as an organic carbon source in an inorganic medium comprising municipal treated water and reverse osmosis (RO) treated wastewater and attained 2.4g/L and 1.6g/L of biomass respectively, as compared to 0.3g/L and 0.16g/L obtained in the wastewaters alone revealing 700% and 900% increase respectively. Mixotrophic regimen cells as analyzed by a 2D Fourier transform infrared (FTIR) spectroscopy for its biochemical content revealed that fog’s blended raw dairy waste (RDW) regimen cells had maximum Carbohydrate/Amide ratio. The study suggests that the mixotrophic regimen C. pyrenoidosa cells can show appropriate growth in a mixture of waste waters and the same comes out to be a cost effective and feasible alternative commercial medium for biomass production without requiring any expensive organic carbon sources in the culture medium.

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Influence of Medium Frequency Light/Dark Cycles on the Cultivation of Auxenochlorella pyrenoidosa

Applied Sciences ◽

10.3390/app10155093 ◽

2020 ◽

Vol 10 (15) ◽

pp. 5093

Author(s):

Zhijie Chen ◽

Bosheng Su

Keyword(s):

Light Intensity ◽

Biomass Production ◽

Cycle Time ◽

Biomass Yield ◽

Continuous Light ◽

Biomass Productivity ◽

Chlorella Pyrenoidosa ◽

Dark Cycle ◽

Medium Frequency ◽

Intermittent Light

Light (wavelength, intensity, and light/dark cycle) have been considered as one of the most important parameters for microalgae cultivation. In this paper, the effect of medium frequency intermittent light on Auxenochlorella pyrenoidosa (formerly Chlorella pyrenoidosa) cultivation was investigated. Three parameters of intermittent light, light intensity, light/dark ratio, and light/dark cycle were employed and the influence of these parameters on the productivity of Auxenochlorella pyrenoidosa was studied. The biomass yield and growth rates were mainly affected by the light fraction and cycle time. Light with 220 μE m−2 s−1 light intensity was determined as the optimal light intensity for biomass production. At the light intensity of 420 μE m−2 s−1, the results indicated that the intermittent light improved the biomass production with larger light/dark ratio compared with the continuous light. At a lower mean light intensity over time, the intermittent light should be more suitable for biomass growth and the decrease in the light/dark ratio (L/D) will lead to a higher biomass productivity. The light/dark cycle time has little influence on the biomass yield.

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