Optimization of Various Growth Media to Freshwater Microalgae for Biomass Production

2011 ◽  
Vol 10 (6) ◽  
pp. 540-545 ◽  
Author(s):  
A. Ilavarasi ◽  
D. Mubarakali ◽  
R. Praveenkum ◽  
E. Baldev ◽  
N. Thajuddin
Keyword(s):  
Biomass Production ◽  
Growth Media ◽  
Freshwater Microalgae

scholarly journals A novel nanoemulsion based microalgal growth medium for enhanced biomass production

2020 ◽  
Author(s):  
Harshita Nigam ◽  
Anushree Malik ◽  
Vikram Singh
Keyword(s):  
Biomass Production ◽  
Biochemical Characterization ◽  
Silicone Oil ◽  
Algal Growth ◽  
Morphological Characteristics ◽  
Biomass Productivity ◽  
Growth Media ◽  
Freshwater Microalgae ◽  
Microalgal Biomass ◽  
And Control

Abstract Background: Microalgae are well-established feedstocks for applications ranging from biofuels to valuable pigments and therapeutic proteins. However, the low biomass productivity using commercially available growth mediums is a roadblock for its mass production. This work describes a strategy to boost algal biomass productivity by using an effective CO2 supplement.Results: In the present study, a novel nanoemulsion-based media has been tested for the growth of freshwater microalgae strain Chlorella pyrenoidosa. Two different nanoemulsion-based media were developed using 1% silicone oil nanoemulsion (1% SE) and 1% paraffin oil nanoemulsion (1% PE) supplemented in Blue-green 11 media (BG11). After 12 days of cultivation, biomass yield was found highest in 1% PE followed by 1% SE and control i.e, 3.20, 2.75, and 1.03 g L-1, respectively. The chlorophyll-a synthesis was improved by 76% in 1% SE and 53% in 1% PE compared with control. The respective microalgal cell numbers for 1% PE, 1% SE and control measured using the cell counter were 3.00 × 106, 2.40 × 106, and 1.34 × 106 cells mL-1. The effective CO2 absorption tendency of the emulsion was highlighted as the key mechanism for enhanced algal growth and biomass production. On the biochemical characterization of the produced biomass, it was found that the nanoemulsion cultivated C. pyrenoidosa had increased lipid (1% PE =26.80%, 1% SE =23.60%) and carbohydrates (1% PE =17.20%, 1% SE =18.90%) content compared to the control (lipid =18.05%, carbohydrates =13.60%).Conclusions: This study describes a novel nanoemulsion which potentially acts as an effective CO2 supplement for microalgal growth media thereby increasing the growth of microalgal cells. Further, nanoemulsions cultivated microalgal biomass depict an increase in lipid and carbohydrate content. The approach provides high microalgal biomass productivity without altering morphological characteristics like cell shape and size as revealed by Field Emission Scanning Electron Microscope (FESEM) images.

scholarly journals A novel nanoemulsion-based microalgal growth medium for enhanced biomass production

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Harshita Nigam ◽  
Anushree Malik ◽  
Vikram Singh
Keyword(s):  
Biomass Production ◽  
Biochemical Characterization ◽  
Silicone Oil ◽  
Algal Growth ◽  
Morphological Characteristics ◽  
Biomass Productivity ◽  
Growth Media ◽  
Freshwater Microalgae ◽  
Microalgal Biomass ◽  
And Control

Abstract Background Microalgae are well-established feedstocks for applications ranging from biofuels to valuable pigments and therapeutic proteins. However, the low biomass productivity using commercially available growth mediums is a roadblock for its mass production. This work describes a strategy to boost algal biomass productivity by using an effective CO2 supplement. Results In the present study, a novel nanoemulsion-based media has been tested for the growth of freshwater microalgae strain Chlorella pyrenoidosa. Two different nanoemulsion-based media were developed using 1% silicone oil nanoemulsion (1% SE) and 1% paraffin oil nanoemulsion (1% PE) supplemented in Blue-green 11 media (BG11). After 12 days of cultivation, biomass yield was found highest in 1% PE followed by 1% SE and control, i.e., 3.20, 2.75, and 1.03 g L−1, respectively. The chlorophyll-a synthesis was improved by 76% in 1% SE and 53% in 1% PE compared with control. The respective microalgal cell numbers for 1% PE, 1% SE and control measured using the cell counter were 3.00 × 106, 2.40 × 106, and 1.34 × 106 cells mL−1. The effective CO2 absorption tendency of the emulsion was highlighted as the key mechanism for enhanced algal growth and biomass production. On the biochemical characterization of the produced biomass, it was found that the nanoemulsion-cultivated C. pyrenoidosa had increased lipid (1% PE = 26.80%, 1% SE = 23.60%) and carbohydrates (1% PE = 17.20%, 1% SE = 18.90%) content compared to the control (lipid = 18.05%, carbohydrates = 13.60%). Conclusions This study describes a novel nanoemulsion which potentially acts as an effective CO2 supplement for microalgal growth media thereby increasing the growth of microalgal cells. Further, nanoemulsion-cultivated microalgal biomass depicts an increase in lipid and carbohydrate content. The approach provides high microalgal biomass productivity without altering morphological characteristics like cell shape and size as revealed by field emission scanning electron microscope (FESEM) images. Graphical abstract

scholarly journals A novel nanoemulsion based microalgal growth medium for enhanced biomass production

2020 ◽  
Author(s):  
Harshita Nigam ◽  
Anushree Malik ◽  
Vikram Singh
Keyword(s):  
Biomass Production ◽  
Biochemical Characterization ◽  
Silicone Oil ◽  
Morphological Characteristics ◽  
Biomass Productivity ◽  
Cell Number ◽  
Growth Media ◽  
Freshwater Microalgae ◽  
Microalgal Biomass ◽  
And Control

Abstract Background: Microalgae are well-established feedstocks for applications ranging from biofuels to valuable pigments and therapeutic proteins. However, the low biomass productivity using commercially available growth mediums is a roadblock for its mass production. This work describes a strategy to boost the algal biomass productivity by using an effective CO 2 supplement.Results: In the present study, a novel nanoemulsion-based media has been tested for the growth of freshwater microalgae strain Chlorella pyrenoidosa. Two different nanoemulsion-based media were developed using 1% silicone oil nanoemulsion (1% SE) and 1% paraffin oil nanoemulsion (1% PE) supplemented in BG11 media During 12-day growth experiment, the 1% PE gave the highest biomass yield (3.2± 0.07gL -1 ), followed by 1%SE (2.75 ± 0.07 gL -1 ) and control (1.03±0.02 gL -1 ). The respective microalgal cell number measured using cell counter were (3.0 ± 0.21 x 10 6 cells ml -1 ), (2.4 ± 0.30 x 10 6 cells ml -1 ) and (1.34 ± 0.09 x 10 6 cells ml -1 ). Cell viability analysis using MTT assay showed that 1% PE also had higher viable cells (94%) compared to 1% SE (77%) and control (53%). The effective CO 2 absorption tendency of the emulsion was highlighted as the key mechanism for greater biomass production. On the biochemical characterization of the produced biomass, it was found that the nanoemulsion cultivated C. pyrenoidosa had increased lipid (1% PE=26.8%, 1% SE=23.6%) and carbohydrates (1% PE=17.2%, 1% SE=18.9%) content compared to the control (lipid=18.05%, carbohydrates=13.6%).Conclusions: This study provides a novel nanoemulsion which acts as an effective CO 2 supplement for microalgal growth media which increase the growth of microalgal cells. Importantly, nanoemulsions cultivated microalgal biomass possess increment in lipid and carbohydrate content. This approach also provides high microalgal biomass productivity without alteration of morphological characteristics like cell shape and cell size.

scholarly journals Growth and biochemical composition of Chlorella vulgaris in different growth media

2013 ◽  
Vol 85 (4) ◽  
pp. 1427-1438 ◽  
Author(s):  
MATHIAS A. CHIA ◽  
ANA T. LOMBARDI ◽  
MARIA DA GRACA G. MELAO
Keyword(s):  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Biochemical Composition ◽  
Physiological Response ◽  
Low Cost ◽  
Cost Effective ◽  
Growth Conditions ◽  
Growth Media ◽  
Continuous Cultures ◽  
The Cost

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

scholarly journals Osmoadaptation Strategy of the Most Halophilic Fungus, Wallemia ichthyophaga, Growing Optimally at Salinities above 15% NaCl

2013 ◽  
Vol 80 (1) ◽  
pp. 247-256 ◽  
Author(s):  
Janja Zajc ◽  
Tina Kogej ◽  
Erwin A. Galinski ◽  
José Ramos ◽  
Nina Gunde-Cimerman
Keyword(s):  
Biomass Production ◽  
Osmotic Shock ◽  
Model Organism ◽  
Salinity Range ◽  
Growth Media ◽  
Optimal Salinity ◽  
Content Type ◽  
Batch Cultures ◽  
Specific Growth Rates ◽  
Optimum Salinity

ABSTRACTWallemia ichthyophagais a fungus from the ancient basidiomycetous genusWallemia(Wallemiales, Wallemiomycetes) that grows only at salinities between 10% (wt/vol) NaCl and saturated NaCl solution. This obligate halophily is unique among fungi. The main goal of this study was to determine the optimal salinity range for growth of the halophilicW. ichthyophagaand to unravel its osmoadaptation strategy. Our results showed that growth on solid growth media was extremely slow and resulted in small colonies. On the other hand, in the liquid batch cultures, the specific growth rates ofW. ichthyophagawere higher, and the biomass production increased with increasing salinities. The optimum salinity range for growth ofW. ichthyophagawas between 15 and 20% (wt/vol) NaCl. At 10% NaCl, the biomass production and the growth rate were by far the lowest among all tested salinities. Furthermore, the cell wall content in the dry biomass was extremely high at salinities above 10%. Our results also showed that glycerol was the major osmotically regulated solute, since its accumulation increased with salinity and was diminished by hypo-osmotic shock. Besides glycerol, smaller amounts of arabitol and trace amounts of mannitol were also detected. In addition,W. ichthyophagamaintained relatively small intracellular amounts of potassium and sodium at constant salinities, but during hyperosmotic shock, the amounts of both cations increased significantly. Given our results and the recent availability of the genome sequence,W. ichthyophagashould become well established as a novel model organism for studies of halophily in eukaryotes.

scholarly journals Exploring an Integrated Manure-Seawater System for Sustainable Cyanobacterial Biomass Production

2019 ◽  
Vol 9 (18) ◽  
pp. 3888
Author(s):  
Sido
Keyword(s):  
Biomass Production ◽  
Photosynthetic Pigment ◽  
Chicken Manure ◽  
Pig Manure ◽  
Growth Media ◽  
Cow Manure ◽  
Salt Tolerant ◽  
Cyanobacterial Strain ◽  
Nacl Concentration ◽  
Cyanobacterial Biomass

Cyanobacterial biomass is important for biofuel and biofertilizer, however, biomass production requires expensive chemical growth nutrients. To address this issue, we explored the use of inexpensive growth nutrient media from an integrated manure-seawater system for cyanobacterial biomass production. Salt-tolerant cyanobacterial strain HSaC and salt-sensitive cyanobacterial strain LC were tested to evaluate the potential of integrated manure-seawater media for sustainable cyanobacterial biomass production. As a prerequisite for seawater experiments, strain HSaC was grown at different NaCl concentrations (0 mM, 60 mM, 120 mM, 180 mM, 240 mM and 300 mM) to identify the optimum salt concentration. The highest biomass yield and photosynthetic pigment contents were obtained at 120 mM NaCl concentration. The highest exo-polysaccharide (EPS) content was obtained at 180 mM NaCl concentration. The treatments for the manure-seawater media were cow manure, pig manure, chicken manure and BG11, each with distilled water, diluted seawater and non-diluted seawater. The highest biomass and photosynthetic pigment yield for cyanobacterial strains LC and HSaC were obtained from 0.5 dS/m and 10 dS/m diluted seawater integrated with cow manure, respectively, but pig and chicken manure performed poorly. Overall, the biomass production and photosynthetic pigment results from cow manure-seawater were relatively better than those from the reference media (BG11). Based on the current findings, it is concluded that the growth nutrients from integrated cow manure-seawater can wholly substitute for the BG11 without affecting cyanobacterial growth, thereby reducing the usage of expensive chemical growth media. Thus, the results of study help to enhance the biomass production of both salt-sensitive and salt-tolerant cyanobacteria for sustainable biofuel and biofertilizer production.

scholarly journals Isolation and selection of growth medium for freshwater microalgae Asterarcys quadricellulare for maximum biomass production

2019 ◽  
Vol 80 (11) ◽  
pp. 2027-2036 ◽  
Author(s):  
Karthikeyan Sangapillai ◽  
Thirumarimurugan Marimuthu
Keyword(s):  
Chlorophyll A ◽  
Growth Medium ◽  
Biomass Production ◽  
Lipid Content ◽  
Dry Weight ◽  
Biofuel Production ◽  
Growth Media ◽  
Chlorophyll B ◽  
Suitable Alternative ◽  
Predominant Strain

Abstract The use of microalgae biomass as a suitable alternative feedstock for biofuel production has been promoted in the field of green biotechnology. In this present study, the microalgae were isolated from freshwater samples. The predominant strain was screened from the samples and grown in four different growth media, including modified Bold's Basal Medium (BBM), modified CFTRI medium, BG11 medium and CHU medium, to find the suitable growth medium to enrich biomass production. In total three microalgae colonies were identified based on their colony morphology microscopically by using a light microscope. The predominant strain was confirmed as Asterarcys quadricellulare using 18S rRNA sequencing. The growth of microalgae was investigated based on parameters like dry weight, pigment composition such as chlorophyll a, chlorophyll b, carotenoid and lipid content in the microalgae. Among the four different media, modified BBM medium showed maximum dry weight (1.44 ± 0.015 g/L), chlorophyll a (23.07 ± 0.049 mg/L), chlorophyll b (16.76 ± 0.010 mg/L), carotenoid (8.92 ± 0.031 mg/L) and lipid content (375 ± 0.020 mg/L) on the 25th day of culture. The gas chromatography mass spectrometry (GC/MS) analysis showed the presence of major fatty acids stearic acid, palmitic acid and oleyl alcohol in the microalgae. Therefore the high lipid content and fatty acid profiles of Asterarcys quadricellulare are becoming a promising suitable strain for biofuel production with modified BBM medium.

scholarly journals EFFECT OF PHYSIOLOGICAL PARAMETERS ON MASS PRODUCTION OF TRICHODERMA SPECIES

2018 ◽  
Vol 30 (1) ◽  
pp. 61
Author(s):  
Muhammad U. Ghazanfar ◽  
Mubashar Raza ◽  
Waqas Raza
Keyword(s):  
Growth Medium ◽  
Biomass Production ◽  
Mass Production ◽  
Environmental Parameters ◽  
Pathogenic Fungi ◽  
Potassium Nitrate ◽  
Effect Of Temperature ◽  
Growth Media ◽  
Trichoderma Species

Mycophagous fungi show antagonistic potential against plant pathogenic fungi and suggested as a promising biocontrol agent that influences by nutritional and environmental parameters. The present research was conducted with the aim to determine optimal environmental and nutritional parameters range for biomass production of Trichoderma. The two species of Trichoderma isolated from rhizosphere of citrus, wheat and tomato of different localities of district Sargodha, Punjab, Pakistan and later on effect of temperature, carbon, nitrogen and pH were investigated on three strain of T. harzianum HM, HK, HC and one strain of T. asperellum TH under in vitro conditions. All strains produced statistically sufficient mass production at all temperatures but 25 °C as well as 30 °C were found ideal for mass production of Trichoderma. A substantial difference in mass production of Trichoderma isolates was recorded at different pH levels includes 4.0, 5.0, 6.0, 7.0 and 8.0. The pH range from 5.0 to 7.0 found to be optimum for all species of Trichoderma. All fungal species of Tricoderma produced extensive mass production on growth media supplemented with carbon and nitrogen sources such as mannose, glactose, sucrose, lactose and sodium nitrite (NaNO3), potassium nitrate (KNO3), ammonium nitrate (NH4NO3) as well as ammonium nitrite (NH4NO3) respectively. The optimal mass production was recorded on carbon supplemented growth medium as compared to nitrogen supplemented growth medium. Therefore, T. harzianum HK produced maximum biomass production on among all isolates of Trichoderma.

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