Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata using high-speed counter-current chromatography

2004 ◽  
Vol 1040 (1) ◽  
pp. 147-149 ◽  
Author(s):  
Qizhen Du ◽  
Ping Chen ◽  
Gerold Jerz ◽  
Peter Winterhalter
Keyword(s):  
High Speed ◽  
Flavonoid Glycosides ◽  
Preparative Separation ◽  
Ampelopsis Grossedentata ◽  
Counter Current

Preparative separation of flavonoid glycosides and flavonoid aglycones from the leaves of Platycladus orientalis by REV-IN and FWD-IN high-speed counter-current chromatography

2019 ◽  
Vol 11 (33) ◽  
pp. 4260-4266
Author(s):  
Qian Liu ◽  
Yanling Geng ◽  
Xiao Wang ◽  
Jia Li ◽  
Jinqian Yu
Keyword(s):  
High Speed ◽  
Flavonoid Glycosides ◽  
Preparative Separation ◽  
Flavonoid Aglycones ◽  
Platycladus Orientalis ◽  
Counter Current ◽  
First Time

Three flavonoid glycosides and four flavonoid aglycones were isolated and purified for the first time from the leaves of Platycladus orientalis via two different elution modes of high-speed counter-current chromatography.

Preparative separation of seven polyphenols from Perillae Folium via pH-zone-refining counter-current chromatography combined with high-speed counter-current chromatography

2021 ◽  
Author(s):  
Lihua Xu ◽  
Tianyu Ma ◽  
Xinming Wang ◽  
Wei Liu ◽  
Jia Li ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
High Speed ◽  
Separation Method ◽  
Flavonoid Glycosides ◽  
Zone Refining ◽  
Preparative Separation ◽  
Crude Sample ◽  
Counter Current

A separation method using pH-ZRCCC combined with HSCCC was proposed to separate the crude sample of Perillae Folium with different constituents and polarity, and three phenolic acids and four flavonoid glycosides were isolated successfully.

scholarly journals Preparative separation of two subsidiary colors of FD&C Yellow No. 5 (Tartrazine) using spiral high-speed counter-current chromatography

2014 ◽  
Vol 1343 ◽  
pp. 91-100 ◽  
Author(s):  
Adrian Weisz ◽  
Clark D. Ridge ◽  
Jose A. Roque ◽  
Eugene P. Mazzola ◽  
Yoichiro Ito
Keyword(s):  
High Speed ◽  
Preparative Separation ◽  
Counter Current

Preparative separation of polyphenols from the flowers of Paeonia lactiflora Pall. by high-speed counter-current chromatography

2014 ◽  
Vol 947-948 ◽  
pp. 62-67 ◽  
Author(s):  
Xikai Shu ◽  
Wenjuan Duan ◽  
Feng Liu ◽  
Xingang Shi ◽  
Yanling Geng ◽  
...  
Keyword(s):  
High Speed ◽  
Preparative Separation ◽  
Paeonia Lactiflora ◽  
Counter Current

scholarly journals Preparative Separation of Diterpene Lactones and Flavones from Andrographis paniculate Using Off-Line Two-Dimensional High-Speed Counter-Current Chromatography

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 620 ◽  
Author(s):  
Xiaowei Sun ◽  
Huijiao Yan ◽  
Yujie Zhang ◽  
Xiao Wang ◽  
Dawei Qin ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
High Speed ◽  
Spectroscopic Methods ◽  
Two Dimensional ◽  
Preparative Separation ◽  
Counter Current ◽  
First Time

Seven diterpene lactones, andrographolide (1), isoandrographolide (2), neo-andrographolide (3), 14-deoxy-11,12-didehydroandrographolide (4), 14-deoxyandrographiside (5), 14-deoxy-11,12-didehydroandrographiside (6), 3,14-dideoxyandrographolide (10), and three flavones, andrographidine C (7), andrographidine A (8), 5-hydroxy-7,8-dimethoxyflavanone (9) have been successfully and efficiently isolated from A. paniculata using an off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) method for the first time. For the first dimension HSCCC separation, petroleum ether-ethyl acetate-methanol-water 3:7:5:5 (v/v) was employed to isolate 14.4 mg of compound 1, 3.1 mg of compound 2, 7.8 mg of compound 3, and 18.0 mg of compound 4 from 200 mg of the A. paniculata extract. For the second dimension HSCCC separation, petroleum ether-ethyl acetate-methanol-water 2:8:1:9 (v/v) and 5:5:6:4 (v/v) were employed to isolate the collected fractions ranged from 55 to 79 min and the flow out fraction, respectively, which led to 5.1 mg of compound 5, 4.4 mg of compound 6, 2.4 mg of compound 7, 3.3 mg of compound 8, 4.0 mg of compound 9, 7.0 mg of compound 10. The structures of these diterpene lactones and flavones were elucidated by extensive spectroscopic methods.

scholarly journals Development of online-storage inner-recycling counter-current chromatography for the preparative separation of complex components of alkylphenols from sarcotesta of Ginkgo biloba L.

RSC Advances ◽  
2018 ◽  
Vol 8 (60) ◽  
pp. 34321-34330 ◽  
Author(s):  
Daijie Wang ◽  
Xiangyun Song ◽  
Huijiao Yan ◽  
Mengmeng Guo ◽  
Ruiming Fu ◽  
...  
Keyword(s):  
Natural Products ◽  
Ginkgo Biloba ◽  
High Speed ◽  
Separation Method ◽  
Preparative Separation ◽  
Online Storage ◽  
Counter Current ◽  
Complex Components

High-speed counter-current chromatography (HSCCC) is becoming an effective and non-absorptive separation method from natural products.

scholarly journals Quick Preparative Separation of Natural Naphthopyranones with Antioxidant Activity by High-Speed Counter-Current Chromatography

2002 ◽  
Vol 57 (11-12) ◽  
pp. 1051-1055 ◽  
Author(s):  
Gilda G. Leitão ◽  
Suzana G. Leitão ◽  
Wagner Vilegas
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
High Speed ◽  
Ethanolic Extract ◽  
Solvent System ◽  
Spectrophotometric Assay ◽  
Preparative Separation ◽  
Dpph Radical ◽  
Preparative Scale ◽  
Counter Current

The natural naphthopyranones paepalantine (1), paepalantine-9O-β-ᴅ-glucopyranoside (2) and paepalantine-9-O-β-ᴅ-allopyranosyl-(1→6)-O-β-ᴅ-glucopyranoside (3) were separated in a preparative scale from the ethanolic extract of the capitula of Paepalanthus bromelioides by high-speed counter-current chromatography (HSCCC). The solvent system used was composed of water-ethanol-ethyl acetate-hexane (10:4 : 10:4, v/v/v/v). This technique led to the separation of the three different naphthopyranone glycosides in pure form in approximately 7 hours. Paepalantine showed a good antioxidant activity when assayed by the DPPH radical spectrophotometric assay.

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