Preparative separation of seven polyphenols from Perillae Folium via pH-zone-refining counter-current chromatography combined with high-speed counter-current chromatography

2021 ◽  
Author(s):  
Lihua Xu ◽  
Tianyu Ma ◽  
Xinming Wang ◽  
Wei Liu ◽  
Jia Li ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
High Speed ◽  
Separation Method ◽  
Flavonoid Glycosides ◽  
Zone Refining ◽  
Preparative Separation ◽  
Crude Sample ◽  
Counter Current

A separation method using pH-ZRCCC combined with HSCCC was proposed to separate the crude sample of Perillae Folium with different constituents and polarity, and three phenolic acids and four flavonoid glycosides were isolated successfully.

scholarly journals Development of online-storage inner-recycling counter-current chromatography for the preparative separation of complex components of alkylphenols from sarcotesta of Ginkgo biloba L.

RSC Advances ◽  
2018 ◽  
Vol 8 (60) ◽  
pp. 34321-34330 ◽  
Author(s):  
Daijie Wang ◽  
Xiangyun Song ◽  
Huijiao Yan ◽  
Mengmeng Guo ◽  
Ruiming Fu ◽  
...  
Keyword(s):  
Natural Products ◽  
Ginkgo Biloba ◽  
High Speed ◽  
Separation Method ◽  
Preparative Separation ◽  
Online Storage ◽  
Counter Current ◽  
Complex Components

High-speed counter-current chromatography (HSCCC) is becoming an effective and non-absorptive separation method from natural products.

scholarly journals Preparative separation of seven phenolic acids from Xanthii Fructus using pH-zone-refining counter-current chromatography combined with semi-preparative high performance liquid chromatography

RSC Advances ◽  
2019 ◽  
Vol 9 (63) ◽  
pp. 36524-36529
Author(s):  
Tianyu Ma ◽  
Lihua Xu ◽  
Xinming Wang ◽  
Jia Li ◽  
Lanping Guo ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
High Performance ◽  
Preparative Hplc ◽  
Zone Refining ◽  
Preparative Separation ◽  
Caffeoylquinic Acid ◽  
Counter Current

Segmentation and purification strategy using pH-zone-refining counter-current chromatography combined with semi-preparative HPLC for separation of six caffeoylquinic acid isomers and a caffeic acid from Xanthii Fructus.

Preparative separation of alkaloids from Litsea cubeba using combined applications of pH-zone-refining and high-speed counter-current chromatography

RSC Advances ◽  
2015 ◽  
Vol 5 (92) ◽  
pp. 75831-75837 ◽  
Author(s):  
Changlei Sun ◽  
Jia Li ◽  
Daijie Wang ◽  
Jinqian Yu ◽  
Xiao Wang ◽  
...  
Keyword(s):  
High Speed ◽  
Zone Refining ◽  
Preparative Separation ◽  
Litsea Cubeba ◽  
Counter Current

pH-zone-refining counter-current chromatography and high-speed counter-current chromatography are used for separation of alkaloids from the extract of Litsea cubeba.

Preparative separation of flavonoid glycosides and flavonoid aglycones from the leaves of Platycladus orientalis by REV-IN and FWD-IN high-speed counter-current chromatography

2019 ◽  
Vol 11 (33) ◽  
pp. 4260-4266
Author(s):  
Qian Liu ◽  
Yanling Geng ◽  
Xiao Wang ◽  
Jia Li ◽  
Jinqian Yu
Keyword(s):  
High Speed ◽  
Flavonoid Glycosides ◽  
Preparative Separation ◽  
Flavonoid Aglycones ◽  
Platycladus Orientalis ◽  
Counter Current ◽  
First Time

Three flavonoid glycosides and four flavonoid aglycones were isolated and purified for the first time from the leaves of Platycladus orientalis via two different elution modes of high-speed counter-current chromatography.

One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

2019 ◽  
Vol 9 (2) ◽  
pp. 138-143
Author(s):  
Tianyun Li ◽  
Xiling Dai ◽  
Yichen Li ◽  
Guozheng Huang ◽  
Jianguo Cao
Keyword(s):  
Natural Products ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Speed ◽  
High Efficiency ◽  
Total Phenolic ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
One Step ◽  
Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

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