Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry

2020 ◽  
Vol 1631 ◽  
pp. 461586
Author(s):  
Rumi Tanoue ◽  
Kazusa Nozaki ◽  
Kei Nomiyama ◽  
Tatsuya Kunisue ◽  
Shinsuke Tanabe
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Rapid Analysis ◽  
Body Tissue ◽  
Whole Body ◽  
Tandem Mass ◽  
Tissue Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Hog Charm II Tetracycline Test Screening Results Compared with a Liquid Chromatography Tandem Mass Spectrometry 10-μg/kg Method

2012 ◽  
Vol 75 (2) ◽  
pp. 405-407 ◽  
Author(s):  
ROBERT SALTER ◽  
STEVEN HOLMES ◽  
DAVID LEGG ◽  
JOEL COBLE ◽  
BRUCE GEORGE
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Limit Of Detection ◽  
Screening Method ◽  
Tandem Mass ◽  
Tissue Samples ◽  
Plant Laboratory ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Pork tissue samples that tested positive and negative by the Charm II tetracycline test screening method in the slaughter plant laboratory were tested with the modified AOAC International liquid chromatography tandem mass spectrometry (LC-MS-MS) method 995.09 to determine the predictive value of the screening method at detecting total tetracyclines at 10 μg/kg of tissue, in compliance with Russian import regulations. There were 218 presumptive-positive tetracycline samples of 4,195 randomly tested hogs. Of these screening test positive samples, 83% (182) were positive, >10 μg/kg by LC-MS-MS; 12.8% (28) were false violative, greater than limit of detection (LOD) but <10 μg/kg; and 4.2% (8) were not detected at the LC-MS-MS LOD. The 36 false-violative and not-detected samples represent 1% of the total samples screened. Twenty-seven of 30 randomly selected tetracycline screening negative samples tested below the LC-MS-MS LOD, and 3 samples tested <3 μg/kg chlortetracycline. Results indicate that the Charm II tetracycline test is effective at predicting hogs containing >10 μg/kg total tetracyclines in compliance with Russian import regulations.

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