Bioanalytical workflow for novel scaffold protein–drug conjugates: quantitation of total Centyrin protein, conjugated Centyrin and free payload for Centyrin–drug conjugate in plasma and tissue samples using liquid chromatography–tandem mass spectrometry

Bioanalysis ◽  
2018 ◽  
Vol 10 (20) ◽  
pp. 1651-1665 ◽  
Author(s):  
Chuan Shi ◽  
Shalom Goldberg ◽  
Tricia Lin ◽  
Vadim Dudkin ◽  
Wayne Widdison ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Protein Drug ◽  
Tandem Mass ◽  
Tissue Samples ◽  
Drug Conjugates ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Novel Scaffold

Hog Charm II Tetracycline Test Screening Results Compared with a Liquid Chromatography Tandem Mass Spectrometry 10-μg/kg Method

2012 ◽  
Vol 75 (2) ◽  
pp. 405-407 ◽  
Author(s):  
ROBERT SALTER ◽  
STEVEN HOLMES ◽  
DAVID LEGG ◽  
JOEL COBLE ◽  
BRUCE GEORGE
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Limit Of Detection ◽  
Screening Method ◽  
Tandem Mass ◽  
Tissue Samples ◽  
Plant Laboratory ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Pork tissue samples that tested positive and negative by the Charm II tetracycline test screening method in the slaughter plant laboratory were tested with the modified AOAC International liquid chromatography tandem mass spectrometry (LC-MS-MS) method 995.09 to determine the predictive value of the screening method at detecting total tetracyclines at 10 μg/kg of tissue, in compliance with Russian import regulations. There were 218 presumptive-positive tetracycline samples of 4,195 randomly tested hogs. Of these screening test positive samples, 83% (182) were positive, >10 μg/kg by LC-MS-MS; 12.8% (28) were false violative, greater than limit of detection (LOD) but <10 μg/kg; and 4.2% (8) were not detected at the LC-MS-MS LOD. The 36 false-violative and not-detected samples represent 1% of the total samples screened. Twenty-seven of 30 randomly selected tetracycline screening negative samples tested below the LC-MS-MS LOD, and 3 samples tested <3 μg/kg chlortetracycline. Results indicate that the Charm II tetracycline test is effective at predicting hogs containing >10 μg/kg total tetracyclines in compliance with Russian import regulations.

scholarly journals Measurement of Breast Tissue Estrogens by Liquid Chromatography-tandem Mass Spectrometry

2021 ◽  
Vol 4 (2) ◽  
pp. e00147
Author(s):  
D.A. Olisov ◽  
V.V Chagovets ◽  
N.L. Starodubtseva ◽  
A.A. Smetnik ◽  
V.V. Rodionov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Breast Tissue ◽  
Tandem Mass ◽  
Ionization Source ◽  
Tissue Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Although estrogen contribution estrogen to breast cancer development is not fully understood, an effective method of their measurement, in the mammary gland might provide additional insight. In this study, we have developed a LC-MS/MS method of simultaneous quantification of estrone and estradiol in breast tissue samples. Analytes were extracted with methyl tert-butyl ether by sonication and derivatized with dansyl chloride. Estrogens were analyzed by liquid chromatography-tandem mass spectrometry with an electrospray ionization source. Accuracy and precision were better than 20% for most concentrations. Although estrone and estradiol levels in normal and malignant breast tissue samples analyzed using our method insignificantly differed. The method developed may be used in further studies aimed at evaluating a role estrogens in breast cancer risk.

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