Research on Separation and Purification of Total Anthraquinones in Radix et Rhizoma Rhei by D301 Macroporous Resin

D301 resin was used to separate and purify total anthraquinones in Radix et Rhizoma Rhei. It was found that the optimized dynamic adsorption conditions is pH7.0, flow velocity of 1.0 BV/h and extracting solution with concentration of 0.3g/mL and optimized dynamic elution conditions is hydrochloric acid with concentration of 0.15mol/L, 75% ethanol, and flow velocity of 1.0 BV/h. Under the above conditions,the average adsorption rate and desorption rate are respectively 89.75% and 91.15%, indicating that extraction efficiency of total anthracenone of emodin is relatively high and that the technology enjoys strong stability.