Interferences of homogentisic acid (HGA) on routine clinical chemistry assays in serum and urine and the implications for biochemical monitoring of patients with alkaptonuria

ABSTRACT Hypertension is an increase of blood pressure over 140/90 mmHg. Hypertensionin along period of timecaninterfere withkidney function. Impaired kidney functioncan be caused byhigh levelsof uricacidin the bloodthatis directly associatedwithperipheralvascular resistanceandrenalvascular. Measurement ofrenal functioncan be measuredbycreatininetest, becauseconcentrations in serumandurineexcretionin24 hoursis relativelyconstant. The aim of this research was to measure and compare creatinine and uric acid on serum and urine in hypertensive and normotensive. The parameters were measured by Mindray BS-300 clinical chemistry analyzer. Ex Post Facto used as method and Cross- sectional used as design. A total of 36 blood and urine samples collected from Hypertensive (N1=18) and normotensive (N2=18) from February to March 2014. SPSS 16.0 was used to analyze the datas, t-test was used to compare value of creatinine urine and uric acid serum while U Mann-Whitney test was used to compare value of creatinine serum. The result of this research showed that the mean value of creatinine serum was 0.80 mg/dL in hypertensive and 0.86 mg/dL in normotensive (p=0.14). The mean value of creatinine urin was 87.28 mg/dL in hypertensive and 74.47 mg/dL in normotensive (p=0.35). The mean value of uric acid serum was 4.06 mg/dL in hypertensive and 4.5 mg/dL in normotensive (p=0.41). The resultsofthe urineuricacidwere foundnegativeofthe presence ofuricacid crystals.In conclusion, there was no different of creatinine and uric acid on serum and urine in hypertensive and normotensive. Keywords: hypertension, normotensive, creatinine, uric acid

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Profiles in altered metabolism. II—Accumulation of homogentisic acid in serum and urine following acetylsalicylic acid ingestion

Journal of Mass Spectrometry ◽

10.1002/bms.1200050503 ◽

1978 ◽

Vol 5 (5) ◽

pp. 331-333 ◽

Cited By ~ 3

Author(s):

J. A. Montgomery ◽

O. A. Mamer

Keyword(s):

Acetylsalicylic Acid ◽

Homogentisic Acid ◽

Altered Metabolism ◽

Serum And Urine

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Electron Microscopic and Isozyme Study of a Case of Ochronosis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100093699 ◽

1972 ◽

Vol 30 ◽

pp. 88-89

Author(s):

Jay W. Cha ◽

Perry J. Melnick

Keyword(s):

Benzene Ring ◽

Enzyme System ◽

Homogentisic Acid ◽

Degenerative Changes ◽

Acid Oxidase ◽

Electron Microscopic ◽

Tyrosine Metabolism ◽

Collagenous Tissues

Hereditary ochronosis in very few cases has been examined electron microscopically or histochemically. In this disease homogentisic acid, a normal intermediary of tyrosine metabolism, forms in excessive amounts. This is believed to be due to absence or defective activity of homogentisic acid oxidase, an enzyme system necessary to break the benzene ring and to further break it down to fumaric and acetoacetic acids. Ochronotic pigment, a polymerized form of homogentisic acid, deposits mainly in mesenchymal tissues. There has been a question whether the pigment originates from the collagenous tissues, or deposits passively, where in contrast to melanin it induces degenerative changes.

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Stimulation of residual insulin secretion by glibenclamide in insulin dependent diabetics

Acta Endocrinologica ◽

10.1530/acta.0.0950372 ◽

1980 ◽

Vol 95 (3) ◽

pp. 372-375 ◽

Cited By ~ 5

Author(s):

B. J. Burke ◽

R. J. Sherriff

Keyword(s):

Insulin Secretion ◽

Insulin Therapy ◽

Metabolic Control ◽

C Peptide ◽

Beneficial Effects ◽

Insulin Dependent ◽

Serum And Urine ◽

Stimulation Of

Abstract. Residual insulin secretion, reflected by the presence of C-peptide in serum and urine, has been demonstrated in 5 of 10 insulin-requiring diabetics of less than 10 years' duration tested. The C-peptide response, in the C-peptide secretors, showed a significant increase in both serum and urine after 4 weeks' treatment with 15 mg glibenclamide daily in addition to their usual insulin regime although no beneficial effects in metabolic control were detected. It is suggested that glibenclamide might be a useful adjunct to insulin therapy in insulinrequiring diabetics who still secrete C-peptide.

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