VARIASI POLA VOKALISASI PADA TAKSONOMI ANAK JENIS ELANG-ULAR (Spilornis cheela) DI PKEK, GARUT, JAWA BARAT

Crested serpent eagle (Spilornis cheela) is a bird of prey with distinctive of uniue vocal. Based on several studies mentioned that there are differences in vocalization at the level of subspecies and is often used to study the role vocalization defining the subspecies in taxonomy. This research aimed to determine the role variation of vocalization pattern in taxonomy on subspecies eagle. The research was conducted from May to September 2017 at Kamojang Eagle Conservation Center. The method used is descriptive method with continuous sampling technique. The samples was an adult eagle from three subspecies serpent eagle. The location of observation determined by purposive sampling with the provisions listening post is less than 5 meters until 30 meters. The data is collected at 7 am to 5 pm. Data were analyzed using sound analysis software. The data taken are fundamental frequency, maximum frequency (MinF), minimum frequency (MinF) and duration. Differences between subspecies were analyzed using Kruskal Wallis and Mann-Whitney U statistical test with SPSS 17.0. The result of the research is The vocalizations can be utilized in the subspecies eagle taxonomy of the species to complement the morphological data, marked by significantly different results on each parameter of vocalization between (Spilornis cheela malayensis) and (Spilornis cheela natunensis). MaxF significantly different in (Spilornis cheela malayensis) and (Spilornis cheela bido), (Spilornis cheela natunensis) and (Spilornis cheela bido).

ISOLASI DAN SELEKSI KHAMIR AMILOLITIK ASAL BUAH NANGKA (Artocarpus heterophyllus Lam.)

The aim of this research is to find the isolates of yeast that have the ability to produce enzyme amylase. The strains that can produce the amylase enzyme are characterized by a clear zone around colonies after addition of iodine solution in medium containing 1% starch soluble. Activity of amylase enzyme can be determined by measuring using spectrophotometer at λ 540 nm. The isolation result obtained 75 representative yeast isolates with colony color white butyrous 16%, white mucoid 29.3%, yellowish white 18.7%, cream 20%, peach mucoid 9.3%, and orange mucoid 6,7%. Screening results showed that 8 isolates were able to produce an amylase enzyme with code isolates K33, K34, K36, K37, K48, K107, and K128. A total of two potential yeast isolates in yielding amylase with K34 and K39 isolate codes had amylolytic index 2.89 and 2.27. The highest enzyme activity was produced by K48 (0.88 U/mL).

PENGARUH INOKULASI RHIZOBIUM TERHADAP PENYERAPAN Cu DAN PERTUMBUHAN KACANG TANAH ( Arachis hypogaea L.)

Penelitian ini dilakukan untuk mengetahui pengaruh Cu terhadap pertumbuhan kacang tanah (Arachis hypogaea L.) yang diinokulasikan dengan rhizobium. Distribusi Cu di organ tanaman juga dianalisa. Penelitian ini dilakukan dengan menggunakan Rancangan Acak Lengkap pola faktorial, masing masing kombinasi perlakuan dengan 6 ulangan. Faktor pertama yaitu konsentrasi /dosis CuSO4 yaitu aplikasi A0 : CuSO4 0 mg/3 kg tanah, A1 : CuSO4 60 mg/30 kg tanah, A2 : CuSO4 90 mg/30 kg tanah, A3 : CuSO4 120 mg/3 kg tanah. Faktor yang kedua yaitu B0 : tanpa inokulasi legin . B1: Inokulasi legin (10 g/kg benih) ,B2 :Inokulasi legin (20 g/kg benih). Parameter pengamatan antara lain kadar Cu pada organ tanaman (batang, akar dan daun), kadar Cu pada biji kacang tanah, berat bintil akar dan pertumbuhan tanaman kacang tanah. Data hasil pengamatan dan pengukuran akan dianalisis secara statistik menggunakan analisis sidik ragam (ANOVA) dan diuji lanjut dengan DMRT (Duncan Multiple Range Test) taraf kepercayaan 95 %. Hasil penelitian menunjukkan bahwa pemberian rhizobium yang dikombinasikan Cu memberi pengaruh yang nyata terhadap parameter pertumbuhan. Dalam jumlah sedikit (60 mg CuSO4/30 kg tanah) mampu meningkatkan pertumbuhan tanaman, tetapi dalam jumlah berlebih menurunkan pertumbuhan tanaman. Cu didistribusikan kesemua organ tanaman termasuk biji. inokulasi legin secara signifikan mengurangi pengaruh hambatan pertumbuhan dan mengabsorpsi kelebihan Cu.tingginya konsentrasi Cu di rhizosfer mengurangi jumlah nodul yang terbentuk. Hasil juga memperlihatkan akumulasi Cu di akar.

DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

UJI KEMAMPUAN ANTAGONISME KHAMIR ASAL DAUN JATI (Tectona grandis) TERHADAP KAPANG PENGKONTAMINAN PADA PAKAN TERNAK AYAM

Antagonism is the interaction that occurs when a microorganism interfere with the growth of other microorganisms. One of the microorganisms that have antagonistic ability is yeast. The research aims to determine the ability of antagonism yeast derived from the leaves of teak (Tectona grandis) as an agent for controling mold from poultry feed is Aspergillus sp., Fusarium sp., and Penicillium sp. Tests carried out using the method of antagonism co-culture in medium Potato Dextrose Broth (PDB) during the four days of incubation temperature of 27-28°C. The results showed that yeasts have antagonism activity against Aspergillus sp., Fusarium sp., and Penicillium sp. shown by mycelial growth inhibition and sporulation. Co-culture method results obtained 5 potential yeast isolates (T3D2 DU 1.7, T4D2 WU2J2, T4D2 DU 2.1, T4D1 DU 2.1, T5D1 DU 2.2).

RESPON AKTIVITAS TIKUS WISTAR JANTAN AKIBAT KONDISI DIET TINGGI SUKROSA DIUKUR MENGGUNAKAN PEREKAM AKTIVITAS

Excessive energi consumption play a role in increasing blood glucose levels (hyperglycemia), due to the inability of the hormone insulin to compensate the high blood glucose levels. Blood glucose receptor found in the hypothalamus of VMH (Ventro Medial Hypothalamus) and LH (the Lateral Hypothalamus) is able to detect and change the settings for the feeding activity. The aim of this study was to obtain the relationship between high sucrose feeding on male Wistar rats of behavior and activity. This method uses Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% sucrose, the second group was given additional feed 40% sucrose, a third group was given additional feed 60% sucrose, and the last as a control group. This feed given continuously for 70 days.Observation of activities conducted using Opto-varimex® auto-track system ver.4.31. The results showed an corellation between activity based doses of sucrose.

EFEKTIVITAS MEDIA PERTUMBUHAN KHAMIR KOMERSIAL (Saccharomyces cerevisiae) UNTUK FERMENTASI BIOETANOL DARI ECENG GONDOK (Eichhornia crassipes)

This study aims to find growth medium commercial yeast (S.cerevisiae) and determine the optimum composition of bioethanol fermentation. This research was conducted at the Laboratory of Bioprocess PPPTMGB “LEMIGAS” along May to September 2015. The method used is experiment using a completely randomized design consisting of two treatment. The first treatment is an alternative growth media utilization, namely, tofu liquid waste, coconut water and a mixture of both. The second treatment is the composition of the fermentation with sugar content of 100 ml, 150 ml and 200 ml with the addition of 10 ml starter in each experiment. Data of commercial yeast cell growth (S.cerevisiae) on alternative growth media were analyzed by Anova one way. The results showed that there was an interaction of commercial yeast cell growth (S.cerevisiae) on alternative growth media. Post-hoc test showed the alternative media that consists of a mixture of tofu liquid waste and coconut water produce the highest commercial yeast cell growth at 25,8 x107 with a 7.62 log value (cells/ml). The most optimum of bioethanol produced in the fermentation process is on sugar 100 ml by the addition of 10 ml starter acquire as much as 45 ml of ethanol content.

ANALISIS BAKTERI Escherichia coli PADA MAKANAN SIAP SAJI DI KANTIN RUMAH SAKIT X DAN KANTIN RUMAH SAKIT Y

The increasing human activity makes the preference for fast food increases. However, some people do not pay attention to the hygiene conditions of food processed from food stalls. Food handlers, equipment utilization, food processing, clean water, and the packaging are the critical points of bacterial contamination. Escherichia coli is a bacterium that usually used as the indicator of food hygiene. The objective of this study is to examine the contamination of coliform bacteria, especially E. coli at two hospital cafeteria by using MPN method and questionnaire regarding the implementation of the basic principles of hygiene. Stages of tests performed that are the presumption test, confirmation test, complementary test, gram stain test, biochemical test IMViC and supported by a questionnaire. From the two locations tested, some samples showed positive result in a presumption test and confirmation test but negatively complementary to biochemical test. This indicates that the sample does not contain E. coli bacteria in food, but there is the possibility of Citrobacter. The negative results of the IMVIC test showed that it is possible bacteria found in the presumption test and confirmation test not E. coli and non-pathogenic bacteria. Based on the results of the questionnaire, most of restaurant owner has understood to served food. Food at the hospital X and Y cafetaria are safe to consume because it has a negative E.coli.

ANALISIS VEGETASI Ficus Racemosa L. DI BANTARAN SUNGAI CILIWUNG WILAYAH PANGADEGAN JAKARTA SELATAN

River is the most important things for the human life, one of the components environmental especially for whom whose living in the city and Ciliwung river is one of the major river beside of others 13 rivers. Bamboo trees, bananas, cotton, gempol and elo are the common flora were dominate plants along the riverbanks (gempol and elo taken from the local people words). Based from interview with some local citizens in the Ciliwung riverbank which pas through the district of Pangadegan (South Jakarta), Ficus racemosa L (elo tree) is the most important plant which can withstand river erosion and could maintained the riverbanks during flood and continued by taking sample that plant on that district for need of analysis and identification. From the calculation Gigantochloa apus types stake has the highest INP value to the over all plot of 65.43% while INP Ficus racemosa is still low, but Ficus racemosa can be one alternative plant for conservation in the Ciliwung river, because Ficus racemosa has strong roots. However the selection of other plants such as bamboo trees is more effective because it has fast growth. Therefore, by combining the types of plants that role as conservation may be a solution.