The human intestinal tract – a hotbed of resistance gene transfer? Part I

2007 ◽  
Vol 29 (3) ◽  
pp. 17-21 ◽  
Author(s):  
Abigail A. Salyers ◽  
Kyung Moon ◽  
David Schlesinger
Keyword(s):  
Gene Transfer ◽  
Resistance Gene ◽  
Intestinal Tract ◽  
Human Intestinal Tract

The human intestinal tract – a hotbed of resistance gene transfer? Part II

2007 ◽  
Vol 29 (4) ◽  
pp. 25-30 ◽  
Author(s):  
Abigail A. Salyers ◽  
Kyung Moon ◽  
David Schlessinger
Keyword(s):  
Gene Transfer ◽  
Resistance Gene ◽  
Intestinal Tract ◽  
Human Intestinal Tract

scholarly journals Resistance Gene Transfer during Treatments for Experimental Avian Colibacillosis

2011 ◽  
Vol 56 (1) ◽  
pp. 189-196 ◽  
Author(s):  
Alexandra Dheilly ◽  
Laëtitia Le Devendec ◽  
Gwenaëlle Mourand ◽  
Axelle Bouder ◽  
Eric Jouy ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Transfer ◽  
Resistance Gene ◽  
Gel Electrophoresis ◽  
Intestinal Tract ◽  
Pulsed Field Gel Electrophoresis ◽  
Content Type ◽  
E Coli ◽  
Plasmid Genes ◽  
Antimicrobial Treatments

ABSTRACTAn experiment was conducted in animal facilities to compare the impacts of four avian colibacillosis treatments—oxytetracycline (OTC), trimethoprim-sulfadimethoxine (SXT), amoxicillin (AMX), or enrofloxacin (ENR)—on the susceptibility ofEscherichia coliin broiler intestinal tracts. Birds were first orally inoculated with rifampin-resistantE. colistrains bearing plasmid genes conferring resistance to fluoroquinolones (qnr), cephalosporins (blaCTX-MorblaFOX), trimethoprim-sulfonamides, aminoglycosides, or tetracyclines. Feces samples were collected before, during, and after antimicrobial treatments. The susceptibilities ofE. colistrains were studied, and resistance gene transfer was analyzed. An increase in the tetracycline-resistantE. colipopulation was observed only in OTC-treated birds, whereas multiresistantE. coliwas detected in the dominantE. colipopulations of SXT-, AMX-, or ENR-treated birds. Most multiresistantE. colistrains were susceptible to rifampin and exhibited various pulsed-field gel electrophoresis profiles, suggesting the transfer of one of the multiresistance plasmids from the inoculated strains to otherE. colistrains in the intestinal tract. In conclusion, this study clearly illustrates how, inE. coli, “old” antimicrobials may coselect antimicrobial resistance to recent and critical molecules.

MAPPING OF MULTIDRUG RESISTANCE GENE 1 AND MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN ISOFORM 1 TO 5 mRNA EXPRESSION ALONG THE HUMAN INTESTINAL TRACT

2004 ◽  
Vol 33 (2) ◽  
pp. 219-224 ◽  
Author(s):  
Christian Zimmermann ◽  
Heike Gutmann ◽  
Petr Hruz ◽  
Jean-Pierre Gutzwiller ◽  
Christoph Beglinger ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Mrna Expression ◽  
Resistance Gene ◽  
Intestinal Tract ◽  
Multidrug Resistance Gene ◽  
Protein Isoform ◽  
Human Intestinal Tract

scholarly journals Human peripheral blood hematopoietic progenitors are optimal targets of retroviral-mediated gene transfer

Blood ◽  
1992 ◽  
Vol 80 (6) ◽  
pp. 1418-1422 ◽  
Author(s):  
M Bregni ◽  
M Magni ◽  
S Siena ◽  
M Di Nicola ◽  
G Bonadonna ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cancer Patients ◽  
Resistance Gene ◽  
Peripheral Blood ◽  
Large Scale ◽  
Human Peripheral Blood ◽  
High Dose ◽  
Hematopoietic Progenitors ◽  
Hematopoietic Stem

Abstract Hematopoietic progenitor cells circulate in the peripheral blood (PB) of cancer patients during the recovery phase that follows treatment with high-dose cyclophosphamide followed by hematopoietic growth factor infusion. We report that when PB progenitors were exposed in vitro to filtered supernatant from cell line PA317-N2, producing amphotropic helper-free N2 vector at conventional titers, successful retroviral- mediated transfer of neomycin resistance gene was documented by polymerase chain reaction in 93% of day 14 myelomonocytic colonies. Under the same conditions, gene transfer was achieved in 22% of steady- state bone marrow-derived myelomonocytic colonies. Neo-resistance gene transfer was documented also in a CD34+/cyclophosphamide-resistant precursor to granulocyte-macrophage colonies, an undifferentiated progenitor close to the hematopoietic stem cell. Neither cocultivation with vector-producing cells nor high vector titer were stringent requisites for efficient gene transfer. The large-scale availability of PB hematopoietic progenitors in cancer patients, together with the high gene transfer rate achieved under safe and clinically feasible conditions, support an optimal approach for gene transfer procedures into the human hematopoietic system.

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