As an important biogenic amine, octopamine plays a role of neurotransmitter in organism. Recent researches revealed that it has an important preventive function on obesity and typeIIdiabetes. As raw materials of food and health food or auxiliary preparation for clinical medicine, octopamine can be used independently or jointly with other materials for weight loss and diabetes treatment. Natural octopamine mainly exists in fish sauce. It is possible to extract octopamine from fish sauce and develop health products. In this study, an effective method was studied to extract octopamine (OA) from fish sauce by means of macroporous adsorption resin (H103) which showed its static saturated adsorption of 2.925 mg·g-1 (dry resin) and high efficiency in separate adsorption. 30% ethanol was as eluent in H103 resin adsorption with its elutive power 98.04%. Macroporous resin H103 after properly treated could be used to extract octopamine. This study also provided the experimental basis for the industrial production of natural octopamine.