Effect of particle functionalization and solution properties on the adsorption of bovine serum albumin and lysozyme onto silica nanoparticles

2020 ◽  
Vol 186 ◽  
pp. 110677 ◽  
Author(s):  
Flávia Elisa Galdino ◽  
Agustin Silvio Picco ◽  
Maurício Luis Sforca ◽  
Mateus Borba Cardoso ◽  
Watson Loh
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Bovine Serum ◽  
Solution Properties

Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly(Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin

2019 ◽  
Vol 16 (10) ◽  
pp. 913-922 ◽  
Author(s):  
Ramprasath Ramakrishnan ◽  
Jolius Gimbun ◽  
Praveen Ramakrishnan ◽  
Balu Ranganathan ◽  
Samala Murali Mohan Reddy ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Salt Content ◽  
Electrospinning Process ◽  
Solution Viscosity ◽  
Operating Parameters ◽  
Solution Properties ◽  
Wire Electrode ◽  
Needleless Electrospinning

Background: This paper presents the effect of solution properties and operating parameters of polyethylene oxide (PEO) based nanofiber using a wire electrode-based needleless electrospinning. Methods: The feed solution was prepared using a PEO dissolved in water or a water-ethanol mixture. The PEO solution is blended with Bovine Serum Albumin protein (BSA) as a model drug to study the effect of the electrospinning process on the stability of the loaded protein. The polymer solution properties such as viscosity, surface tension, and conductivity were controlled by adjusting the solvent and salt content. The morphology and fiber size distribution of the nanofiber was analyzed using scanning electron microscopy. Results: The results show that the issue of a beaded nanofiber can be eliminated either by increasing the solution viscosity or by the addition of salt and ethanol to the PEO-water system. The addition of salt and solvent produced a high frequency of smaller fiber diameter ranging from 100 to 150 nm. The encapsulation of BSA in PEO nanofiber was characterized by three different spectroscopy techniques (i.e. circular dichroism, Fourier transform infrared, and fluorescence) and the results showed the BSA is well encapsulated in the PEO matrix with no changes in the protein structure. Conclusion: This work may serve as a useful guide for a drug delivery industry to process a nanofiber at a large and continuous scale with a blend of drugs in nanofiber using a wire electrode electrospinning.

An ultrasensitive nanobiohybrid platform for glucose electrochemical biosensing based on ferrocenyl iminopropyl-modified silica nanoparticles

RSC Advances ◽  
2016 ◽  
Vol 6 (52) ◽  
pp. 46238-46243 ◽  
Author(s):  
M. Saadaoui ◽  
M. Braiek ◽  
N. Jaffrezic-Renault ◽  
N. Raouafi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Glucose Oxidase ◽  
Bovine Serum ◽  
Silica Nanoparticle ◽  
Modified Silica ◽  
Electrochemical Biosensing ◽  
Modified Silica Nanoparticles

A novel nanobiohybrid platform based on ferrocenyl iminopropyl-modified silica nanoparticle conjugates (fap-SiNPs), entrapped in glucose oxidase (GOx) and bovine serum albumin cross-linked with glutaraldehyde, was developed.

N-acetylcysteine delivery with silica nanoparticles into 3T3-L1 adipocytes

2021 ◽  
Author(s):  
Evelyn Frontera ◽  
Martin F Desimone ◽  
Mauricio C De Marzi ◽  
Liliana N Guerra
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Cellular Uptake ◽  
Bovine Serum ◽  
Cytotoxic Effect ◽  
Cellular Accumulation ◽  
Dose Dependent

Background: The addition of 5 mM N-acetylcysteine (NAC) to 3T3-L1 adipocytes culture inhibits the accumulation of triglycerides (Tg) by 50%, but after 48 h uptake was only 16% of total NAC available. Based on these results, the aim of this study is to increase the NAC cellular uptake by encapsulating it in silica nanoparticles (NPs). Materials & methods: Silica NPs, 20 ± 4.5 nm in size, were developed, with an inner cavity loaded with 5 mM NAC. At 48 h after treatment, there was a dose-dependent cytotoxic effect. We attempted to reduce the cytotoxicity of silica NPs by coating them with bovine serum albumin. Results: While we obtained nontoxic bovine serum albumin coated NPs, their effect on Tg cellular accumulation was also reduced.

scholarly journals Interactions between bovine serum albumin and mesoporous silica nanoparticles functionalized with biopolymers

2018 ◽  
Vol 340 ◽  
pp. 42-50 ◽  
Author(s):  
Valentina Nairi ◽  
Sara Medda ◽  
Marco Piludu ◽  
Maria Francesca Casula ◽  
Maria Vallet-Regì ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Mesoporous Silica ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Bovine Serum ◽  
Mesoporous Silica Nanoparticles

Enantiomer separation of propranolol and tryptophan using bovine serum albumin functionalized silica nanoparticles as adsorbents

RSC Advances ◽  
2015 ◽  
Vol 5 (114) ◽  
pp. 93850-93857 ◽  
Author(s):  
Wei Li ◽  
Guo-Sheng Ding ◽  
An-Na Tang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Chiral Separation ◽  
Bovine Serum ◽  
Enantiomer Separation ◽  
Functionalized Silica ◽  
Separation Science ◽  
Chiral Selectors ◽  
Functionalized Silica Nanoparticles

The immobilization of popular chiral selectors on the surface of nanomaterials to prepare new chiral adsorbents for preparative chiral separation is a research hotspot in separation science nowadays.

Sign in / Sign up

Export Citation Format

Share Document