N-acetylcysteine delivery with silica nanoparticles into 3T3-L1 adipocytes

2021 ◽  
Author(s):  
Evelyn Frontera ◽  
Martin F Desimone ◽  
Mauricio C De Marzi ◽  
Liliana N Guerra
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Cellular Uptake ◽  
Bovine Serum ◽  
Cytotoxic Effect ◽  
Cellular Accumulation ◽  
Dose Dependent

Background: The addition of 5 mM N-acetylcysteine (NAC) to 3T3-L1 adipocytes culture inhibits the accumulation of triglycerides (Tg) by 50%, but after 48 h uptake was only 16% of total NAC available. Based on these results, the aim of this study is to increase the NAC cellular uptake by encapsulating it in silica nanoparticles (NPs). Materials & methods: Silica NPs, 20 ± 4.5 nm in size, were developed, with an inner cavity loaded with 5 mM NAC. At 48 h after treatment, there was a dose-dependent cytotoxic effect. We attempted to reduce the cytotoxicity of silica NPs by coating them with bovine serum albumin. Results: While we obtained nontoxic bovine serum albumin coated NPs, their effect on Tg cellular accumulation was also reduced.

An ultrasensitive nanobiohybrid platform for glucose electrochemical biosensing based on ferrocenyl iminopropyl-modified silica nanoparticles

RSC Advances ◽  
2016 ◽  
Vol 6 (52) ◽  
pp. 46238-46243 ◽  
Author(s):  
M. Saadaoui ◽  
M. Braiek ◽  
N. Jaffrezic-Renault ◽  
N. Raouafi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Glucose Oxidase ◽  
Bovine Serum ◽  
Silica Nanoparticle ◽  
Modified Silica ◽  
Electrochemical Biosensing ◽  
Modified Silica Nanoparticles

A novel nanobiohybrid platform based on ferrocenyl iminopropyl-modified silica nanoparticle conjugates (fap-SiNPs), entrapped in glucose oxidase (GOx) and bovine serum albumin cross-linked with glutaraldehyde, was developed.

Effect of particle functionalization and solution properties on the adsorption of bovine serum albumin and lysozyme onto silica nanoparticles

2020 ◽  
Vol 186 ◽  
pp. 110677 ◽  
Author(s):  
Flávia Elisa Galdino ◽  
Agustin Silvio Picco ◽  
Maurício Luis Sforca ◽  
Mateus Borba Cardoso ◽  
Watson Loh
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Bovine Serum ◽  
Solution Properties

scholarly journals Metabolism of platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and lyso-PAF (1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine) by cultured rat Kupffer cells

1989 ◽  
Vol 261 (1) ◽  
pp. 77-81 ◽  
Author(s):  
W Chao ◽  
A Siafaka-Kapadai ◽  
D J Hanahan ◽  
M S Olson
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Cellular Uptake ◽  
Kupffer Cells ◽  
Bovine Serum ◽  
Platelet Activating Factor ◽  
Metabolic Product ◽  
Supernatant Fraction ◽  
Substantial Influence ◽  
Subsequent Formation

The metabolism of platelet-activating factor (PAF; identified as AGEPC: 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and lyso-PAF (lyso-GEPC: 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine) was investigated in cultured rat Kupffer cells. The rat Kupffer cells accumulated [3H]AGEPC and deacetylated this compound to the corresponding [3H]lyso-GEPC, which was the major metabolic product of [3H]AGEPC. [3H]Lyso-GEPC was distributed primarily in the supernatant fraction of incubated cells throughout the experimental interval. Only a very small portion of the [3H]lyso-GEPC was further converted to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (alkylacyl-GPC), indicating that this acylation process was not particularly active in these cells. When [3H]lyso-GEPC was incubated with Kupffer cells, the conversion of lyso-GEPC to AGEPC via the acetyltransferase reaction increased up to 30 min and declined thereafter. Bovine serum albumin (BSA) had a substantial influence on both the cellular uptake and the metabolism of [3H]AGEPC. An increase in the BSA concentration in the incubation media reduced the cellular uptake of [3H]AGEPC and the subsequent formation of lyso-GEPC. The results of this study suggest that the hepatic Kupffer cells play an important role in the metabolism of PAF. Moreover, these results infer that the regulation of the PAF level in certain hepatic pathophysiological situations may be a consequence of the production and subsequent metabolism of this potent lipid autacoid in the Kupffer cells of the liver.

scholarly journals Interactions between bovine serum albumin and mesoporous silica nanoparticles functionalized with biopolymers

2018 ◽  
Vol 340 ◽  
pp. 42-50 ◽  
Author(s):  
Valentina Nairi ◽  
Sara Medda ◽  
Marco Piludu ◽  
Maria Francesca Casula ◽  
Maria Vallet-Regì ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Mesoporous Silica ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Bovine Serum ◽  
Mesoporous Silica Nanoparticles

Effects of Cereal Prolamin Peptides on Differentiated CaCo-2 Cells

1996 ◽  
Vol 24 (4) ◽  
pp. 547-552
Author(s):  
Claudio Giovannini ◽  
Roberto Luchetti ◽  
Elena Mancini ◽  
Massimo de Vincenzi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Cell Viability ◽  
Mtt Assay ◽  
Bovine Serum ◽  
Cytotoxic Effect ◽  
Cell Damage ◽  
Inhibitory Effect ◽  
Culture Cell ◽  
Glycoprotein Synthesis

The effects of peptic-tiyptic (PT) digests of prolamins derived from several cereals on differentiated CaCo-2 cells were studied on the nineteenth day of culture. Cell viability was determined by using the MTT assay and the colony-forming ability method. The metabolic consequences of peptide exposure were evaluated by measuring RNA, protein and glycoprotein synthesis. While PT digests from bovine serum albumin and durum wheat did not exert any effects, those derived from bread wheat, barley, rye and oats caused a dramatic inhibitory effect on metabolic synthesis and, when measured by using the colony-forming technique, a decrease in cell viability. The MTT assay did not indicate any changes in cell viability. These observations support the hypothesis that, although prolamin-derived peptides from these cereals do not exert an immediate cytotoxic effect, they are responsible for cell damage by impairment of metabolic processes.

Enantiomer separation of propranolol and tryptophan using bovine serum albumin functionalized silica nanoparticles as adsorbents

RSC Advances ◽  
2015 ◽  
Vol 5 (114) ◽  
pp. 93850-93857 ◽  
Author(s):  
Wei Li ◽  
Guo-Sheng Ding ◽  
An-Na Tang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Chiral Separation ◽  
Bovine Serum ◽  
Enantiomer Separation ◽  
Functionalized Silica ◽  
Separation Science ◽  
Chiral Selectors ◽  
Functionalized Silica Nanoparticles

The immobilization of popular chiral selectors on the surface of nanomaterials to prepare new chiral adsorbents for preparative chiral separation is a research hotspot in separation science nowadays.

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