Influence of silver speciation on the inflammatory regulation of AgNPs anchoring onto titania nanotubes

Patient material from rare diseases such as very early-onset inflammatory bowel disease (VEO-IBD) is often limited. The use of patient-derived induced pluripotent stem cells (iPSCs) for disease modeling is a promising approach to investigate disease pathomechanisms and therapeutic strategies. We successfully developed VEO-IBD patient-derived iPSC lines harboring a mutation in the IL-10 receptor β-chain (IL-10RB) associated with defective IL-10 signaling. To characterize the disease phenotype, healthy control and VEO-IBD iPSCs were differentiated into macrophages. IL-10 stimulation induced characteristic signal transducer and activator of transcription 3 (STAT3) and suppressor of cytokine signaling 3 (SOCS3) downstream signaling and anti-inflammatory regulation of lipopolysaccharide (LPS)-mediated cytokine secretion in healthy control iPSC-derived macrophages. In contrast, IL-10 stimulation of macrophages derived from patient iPSCs did not result in STAT3 phosphorylation and subsequent SOCS3 expression, recapitulating the phenotype of cells from patients with IL-10RB deficiency. In line with this, LPS-induced cytokine secretion (e.g., IL-6 and tumor necrosis factor-α (TNF-α)) could not be downregulated by exogenous IL-10 stimulation in VEO-IBD iPSC-derived macrophages. Correction of the IL-10RB defect via lentiviral gene therapy or genome editing in the adeno-associated virus integration site 1 (AAVS1) safe harbor locus led to reconstitution of the anti-inflammatory response. Corrected cells showed IL-10RB expression, IL-10-inducible phosphorylation of STAT3, and subsequent SOCS3 expression. Furthermore, LPS-mediated TNF-α secretion could be modulated by IL-10 stimulation in gene-edited VEO-IBD iPSC-derived macrophages. Our established disease models provide the opportunity to identify and validate new curative molecular therapies and to investigate phenotypes and consequences of additional individual IL-10 signaling pathway-dependent VEO-IBD mutations.

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Study of Ce, Sb, and Y exchanged titania nanotubes and superior catalytic performance for the selective catalytic reduction of NOx

Applied Catalysis B Environmental ◽

10.1016/j.apcatb.2021.119939 ◽

2021 ◽

Vol 287 ◽

pp. 119939

Author(s):

Devaiah Damma ◽

Dimitrios K. Pappas ◽

Thirupathi Boningari ◽

Panagiotis G. Smirniotis

Keyword(s):

Selective Catalytic Reduction ◽

Catalytic Reduction ◽

Catalytic Performance ◽

Titania Nanotubes ◽

Reduction Of Nox

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Highly active CoMoS phase on titania nanotubes as new hydrodesulfurization catalysts

Applied Catalysis B Environmental ◽

10.1016/j.apcatb.2009.03.024 ◽

2009 ◽

Vol 90 (1-2) ◽

pp. 213-223 ◽

Cited By ~ 41

Author(s):

J.A. Toledo-Antonio ◽

M.A. Cortés-Jácome ◽

C. Angeles-Chávez ◽

J. Escobar ◽

M.C. Barrera ◽

...

Keyword(s):

Titania Nanotubes ◽

Highly Active ◽

Hydrodesulfurization Catalysts

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Effects of anodization parameters on the formation of titania nanotubes in ethylene glycol

Electrochimica Acta ◽

10.1016/j.electacta.2010.10.004 ◽

2010 ◽

Vol 56 (2) ◽

pp. 905-912 ◽

Cited By ~ 48

Author(s):

Z.B. Xie ◽

D.J. Blackwood

Keyword(s):

Ethylene Glycol ◽

Titania Nanotubes

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Study on Synthesis and Photocatalytic Activity of Porous Titania Nanotubes

Advances in Materials Science and Engineering ◽

10.1155/2016/3532817 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Huang Liu ◽

Yanhua Zhang ◽

Hongtao Yang ◽

Wei Xiao ◽

Lanlan Sun

Keyword(s):

Electron Microscopy ◽

Photocatalytic Activity ◽

Filter Paper ◽

Photoelectron Spectroscopy ◽

Diffuse Reflectance Spectroscopy ◽

Cellulose Nanofibers ◽

Titania Nanotubes ◽

Tetrabutyl Titanate ◽

X Ray ◽

Porous Titania

Using the common natural cellulose substance (filter paper) and triblock copolymer (Pluronic P123) micelles as dual templates, porous titania nanotubes with enhanced photocatalytic activity have been successfully synthesized through sol-gel methods. Firstly, P123 micelles were adsorbed onto the surfaces of cellulose nanofibers of filter paper, followed by hydrolysis and condensation of tetrabutyl titanate around these micelles to form titania layer. After calcination to remove the organic templates, hierarchical titania nanotubes with pores in the walls were obtained. The sample was characterized by X-ray diffraction pattern (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), nitrogen adsorption/desorption, Fourier Transform Infrared Spectroscopy (FT-IR), Ultraviolet-Visible Diffuse Reflectance Spectroscopy (UV-Vis DRS), and X-ray photoelectron spectroscopy (XPS). As compared with commercial P25 catalyst, the porous titania nanotubes prepared by this method displayed significantly enhanced photocatalytic activity for degrading methyl orange under UV irradiation. Within 10 minutes, the porous titania nanotubes are able to degrade over 70% of the original MO, while the value for the commercial Degussa P25 is only about 33%.

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