The in vitro effect of emergency contraception doses of levonorgestrel on the acrosome reaction of human spermatozoa

Contraception ◽  
2005 ◽  
Vol 72 (3) ◽  
pp. 225-228 ◽  
Author(s):  
Karen Saboya Brito ◽  
Luis Bahamondes ◽  
Josiane A.A. Nascimento ◽  
Luciana de Santis ◽  
María José Munuce
Keyword(s):  
Emergency Contraception ◽  
Acrosome Reaction ◽  
Human Spermatozoa ◽  
Vitro Effect

The in vitro effect of levonorgestrel on the acrosome reaction of human spermatozoa from fertile men

Contraception ◽  
2003 ◽  
Vol 68 (1) ◽  
pp. 55-59 ◽  
Author(s):  
L Bahamondes ◽  
J.A.A Nascimento ◽  
María José Munuce ◽  
F Fazano ◽  
A Faúndes
Keyword(s):  
Acrosome Reaction ◽  
Human Spermatozoa ◽  
Vitro Effect

In vitro effect of autologous platelet‐rich plasma on H 2 O 2 ‐induced oxidative stress in human spermatozoa

Andrology ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 191-200 ◽  
Author(s):  
R. Bader ◽  
J. N. Ibrahim ◽  
M. Moussa ◽  
A. Mourad ◽  
J. Azoury ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Platelet Rich Plasma ◽  
Human Spermatozoa ◽  
Autologous Platelet Rich Plasma ◽  
Vitro Effect ◽  
Autologous Platelet

scholarly journals Uterine flushings from women treated with levonorgestrel affect sperm functionality in vitro

Reproduction ◽  
2017 ◽  
Vol 154 (5) ◽  
pp. 607-614 ◽  
Author(s):  
Mayel Chirinos ◽  
Marta Durand ◽  
María Elena González-González ◽  
Gabriela Hernández-Silva ◽  
Israel Maldonado-Rosas ◽  
...  
Keyword(s):  
Tyrosine Phosphorylation ◽  
Emergency Contraception ◽  
Acrosome Reaction ◽  
Binding Capacity ◽  
Human Sperm ◽  
Contraceptive Efficacy ◽  
Serum Luteinizing Hormone ◽  
Emergency Contraceptive ◽  
Testosterone Derivative

Levonorgestrel (LNG), a synthetic 19 nor-testosterone derivative, is widely used for emergency contraception. It is well known that LNG prevents ovulation only when given prior to the surge of serum luteinizing hormone (LH) during the periovulatory phase of the menstrual cycle. This observation suggests that LNG, given its contraceptive efficacy, has additional effects other than those affecting ovulation. In this study, we have evaluated the effects on human sperm functionality of uterine flushings (UF) obtained from women at day LH + 1 of a control cycle (CTR-LH + 1) and after receiving LNG (LNG-LH + 1) two days before the surge of LH. Human sperm from normozoospermic donors were incubated with UF and protein tyrosine phosphorylation, sperm motility, acrosome reaction as well as zona pellucida (ZP) binding capacity were assessed. A significant decrease in total motility and tyrosine phosphorylation accompanied by an increase on spontaneous acrosome reaction was observed when sperm were incubated in the presence of LNG-LH + 1. None of these effects were mimicked by purified glycodelin A (GdA). Moreover, the addition of UF obtained during the periovulatory phase from LNG-treated women or the presence of purified GdA significantly decreased sperm-ZP binding. The data were compatible with changes affecting sperm capacitation, motility and interaction with the ZP. These results may offer evidence on additional mechanisms of action of LNG as an emergency contraceptive.

The in vitro effect of benzo[a]pyrene on human sperm hyperactivation and acrosome reaction

2010 ◽  
Vol 94 (2) ◽  
pp. 595-598 ◽  
Author(s):  
Dyutiman Mukhopadhyay ◽  
Parag Nandi ◽  
Alex C. Varghese ◽  
Rohit Gutgutia ◽  
Samir Banerjee ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Vitro Effect

The diagnostic significance of the induced acrosome reaction

1994 ◽  
Vol 3 (3) ◽  
pp. 159-178 ◽  
Author(s):  
Christopher J De Jonge
Keyword(s):  
Male Infertility ◽  
Acrosome Reaction ◽  
Culture Conditions ◽  
Human Spermatozoa ◽  
Future Application ◽  
Clinical Implications ◽  
General Description ◽  
Diagnostic Significance ◽  
Comprehensive Literature Review

In trying to discern potential causes of male infertility, little investigation has been done to determine if there is predictive value in assessing acrosome reaction inducibility of human spermatozoa for clinical interpretation and application. The purpose of this review is to outline what is currently known in terms of the diagnostic significance of the induced acrosome reaction and to discuss what might be anticipated for the future. In constructing this review, every effort has been made to provide a comprehensive literature review for the reader and a number of review articles have been cited to serve as sources for additional related material. Regarding the aforementioned, it is essential to qualify that, with the exception of the Section entitled: ‘General characteristics of capacitation and the acrosome reaction’, only characteristics, properties and areas relevant to human spermatozoa will be addressed. Thus, in total, this review will: (1) provide a general description of capacitation and the acrosome reaction; (2) briefly characterize the acrosome and its properties; (3) address the signalling mechanism(s) by which the acrosome reaction occurs; (4) outline various methods for detecting the acrosome reaction; (5) define in vitro culture conditions that facilitate the acrosome reaction; (6) define agents, i.e., biological and chemical, that induce the reaction; (7) discuss the association of the acrosome reaction with fertilization; (8) discuss the clinical implications of the induced acrosome reaction; (9) discuss the relevance of sperm function assays in general; and lastly, (10) discuss future application of acrosome reaction assessment for the clinical diagnosis of male infertility.

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