Living Cryopreserved Bone Allograft as an Adjunct for Hindfoot Arthrodesis

2018 ◽  
Vol 35 (3) ◽  
pp. 295-310 ◽  
Author(s):  
Mark K. Magnus ◽  
Kelli L. Iceman ◽  
Thomas S. Roukis
Keyword(s):  
Bone Allograft ◽  
Hindfoot Arthrodesis

scholarly journals Initial study on removing cellular residues from hydrostatic high-pressure treated allogeneic tissue using ultrasound

2020 ◽  
Vol 6 (3) ◽  
pp. 176-179
Author(s):  
Christoph Drobek ◽  
Janine Waletzko ◽  
Michael Dau ◽  
Bernhard Frerich ◽  
Volker Weißmann ◽  
...  
Keyword(s):  
High Pressure ◽  
Structural Properties ◽  
Dna Content ◽  
Bone Allograft ◽  
Initial Study ◽  
Microbiological Contamination ◽  
Test Setup ◽  
Allograft Tissue ◽  
Bone Allografts ◽  
Content Determination

AbstractHydrostatic high-pressure technology (HHD) devitalizes tissue quickly and gently, without negatively affecting the structural properties. HHD-treated tissues must be cleaned from devitalized cells. A partially automated, gentle, reproducible and timesaving rinsing test setup utilizing ultrasound is demonstrated in this study. The test setup is used to clean HHD-treated bone allografts of tissue residues and prevent microbiological contamination. A rinsing procedure is investigated. Residual DNA content determination is utilized to analyze cleaned bone allograft tissue for rinsing procedure evaluation.

scholarly journals Volumetric changes in edentulous alveolar ridge sites utilizing guided bone regeneration and a custom titanium ridge augmentation matrix (CTRAM): a case series study

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Craig E. Hofferber ◽  
J. Cameron Beck ◽  
Peter C. Liacouras ◽  
Jeffrey R. Wessel ◽  
Thu P. Getka
Keyword(s):  
Case Series ◽  
Bone Allograft ◽  
Volumetric Analysis ◽  
Collagen Membrane ◽  
Ridge Augmentation ◽  
Implant Placement ◽  
Case Series Study ◽  
Freeze Dried ◽  
Clinical Measurements ◽  
Bone Gain

Abstract Background The purpose of this study was to evaluate the volumetric changes in partially edentulous alveolar ridges augmented with customized titanium ridge augmentation matrices (CTRAM), freeze-dried bone allograft, and a resorbable collagen membrane. Methods A pre-surgical cone beam computed tomography (CBCT) scan was obtained for CTRAM design/fabrication and to evaluate pre-surgical ridge dimensions. Ridge augmentation surgery using CTRAM, freeze-dried bone allograft, and a resorbable collagen membrane was performed at each deficient site. Clinical measurements of the area of augmentation were made at the time of CTRAM placement and re-entry, and a 2nd CBCT scan 7 months after graft placement was used for volumetric analysis. Locations of each CTRAM in situ were also compared to their planned positions. Re-entry surgery and implant placement was performed 8 months after CTRAM placement. Results Nine subjects were treated with CTRAM and freeze-dried bone allograft. Four out of the nine patients enrolled (44.4%) experienced premature CTRAM exposure during healing, and in two of these cases, CTRAM were removed early. Early exposure did not result in total graft failure in any case. Mean volumetric bone gain was 85.5 ± 30.9% of planned augmentation volume (61.3 ± 33.6% in subjects with premature CTRAM exposure vs. 104.9% for subjects without premature exposure, p = 0.03). Mean horizontal augmentation (measured clinically) was 3.02 mm, and vertical augmentation 2.86 mm. Mean surgical positional deviation of CTRAM from the planned location was 1.09 mm. Conclusion The use of CTRAM in conjunction with bone graft and a collagen membrane resulted in vertical and horizontal bone gain suitable for implant placement.

scholarly journals Forearm reconstruction with bone allograft following tumor excision: A series of 10 patients with a mean follow-up of 10 years

2011 ◽  
Vol 97 (8) ◽  
pp. 793-799 ◽  
Author(s):  
T. van Isacker ◽  
O. Barbier ◽  
A. Traore ◽  
O. Cornu ◽  
F. Mazzeo ◽  
...  
Keyword(s):  
Bone Allograft ◽  
Tumor Excision

scholarly journals Use of Supercritical Sterilized Bone Allograft in Two Stage Revision ACL Reconstruction

2016 ◽  
Vol 4 (2_suppl) ◽  
pp. 2325967116S0002
Author(s):  
Justin Roe ◽  
Sjoerd Rutten ◽  
Fiona Bonnar ◽  
Lucy Salmon ◽  
Leo Pinczewski
Keyword(s):  
Acl Reconstruction ◽  
Bone Allograft ◽  
Two Stage ◽  
Revision Acl Reconstruction

Failure of Maxillary Reconstruction With Freeze-Dried Bone Allograft

2016 ◽  
Vol 27 (3) ◽  
pp. e301-e302
Author(s):  
María del Pilar Rodríguez-Sánchez ◽  
Járede C. Pereira ◽  
Idelmo Rangel Garcia Júnior
Keyword(s):  
Bone Allograft ◽  
Freeze Dried

scholarly journals Alveolar Ridge Preservation After Tooth Extraction with DFDBA and Platelet Concentrates: A Radiographic Retrospective Study

2017 ◽  
Vol 11 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Behrang Baniasadi ◽  
Laurence Evrard
Keyword(s):  
Bone Loss ◽  
Tooth Extraction ◽  
Alveolar Bone ◽  
Bone Allograft ◽  
Ridge Preservation ◽  
Platelet Concentrates ◽  
Freeze Dried ◽  
Significant Difference ◽  
Removable Prosthesis ◽  
The Mean

Objectives: The purpose of this study was to evaluate vertical alveolar bone loss 3 months after tooth extraction when a technique of ridge preservation was applied using a particulate demineralized freeze-dried bone allograft 300 - 500 µm associated with platelet concentrates (platelet-rich-fibrin) in the form of gel and membranes. Material and Methods: A retrospective radiological clinical study was conducted on 56 patients for whom 95 extractions had been performed immediately followed by alveolar filling. Among the patients, 17 were smokers and 16 were provided with an immediate removable temporary prosthesis after extractions. Vertical bone loss was measured radiologically by panoramic X-ray before extractions and by a computed tomography scan 3 months after, at the level of mid-buccal bone wall, by two independent observers. For statistical analysis, Student’s t-test was performed to compare the mean bone loss between mono- and pluri-radicular teeth and to compare the mean bone loss between tobacco users versus non users and finally to compare the mean bone loss between individuals that had provisional removable prosthesis and those that had not. Results: Three months after tooth extraction, the mean of vertical loss of the mid-buccal bone wall was 0.72 (SD 0.71) mm (5.53% SD 5.19). No significant difference between bone loss at mono-radicular and pluri-radicular teeth (P = 0.982) was observed. There was no significant correlation between tobacco habits and bone loss (P = 0.2), nor between provisional removable prosthesis and bone loss (P = 0.786). Conclusion: These results indicate a good potential for the technique using Demineralized Freeze-Dried Bone Allograft 300 - 500 µm and platelet concentrates in alveolar bone preservation.

Evaluation of Human Periodontal Ligament and Osteosarcoma Cell Attachment and Viability on Particulate Bone and Dentin Allografts

2020 ◽  
pp. 1-6
Keyword(s):  
Particle Size ◽  
Cell Lines ◽  
Bone Grafting ◽  
Periodontal Ligament ◽  
Cell Attachment ◽  
Bone Allograft ◽  
Osteosarcoma Cell ◽  
Cellular Attachment ◽  
Freeze Dried ◽  
Demineralized Dentin

Purpose: Various bone grafting substitutes have been used in the periodontics for bone regeneration which include autografts, allografts, xenografts and alloplasts. Autogenous particulate dentin has been used successfully as a bone grafting substitute. The aim of present study was to evaluate the effect of demineralized and mineralized freeze-dried bone allograft and particulate dentin on osteoblasts-like cells and human periodontal fibroblasts. Materials and methods: Demineralized freeze-dried bone allograft (DFDBA) and freeze-dried bone allograft (FDBA) and ground dentin was used in the study. Particulate dentin was divided into four groups according to the size of the particles and demineralization - small dentin (particle size less than 200 µm), small dentin demineralized, large dentin (particle size 250-1200 µm), large dentin demineralized. Effect of all the specimens was checked on osteoblast-like cells (MG63) and human periodontal ligament cell lines. Percentage of surviving cells was measured using colorimetric MTT assay spectrophotometrically on 7th and 14th day of the cell culture. Scanning electron microscopy (SEM) was used to check the cellular attachment. Results: Demineralized dentin matrix has shown significantly enhanced viable cell percentage for both the cell lines. DFDBA and demineralized dentin has reported comparable percentage of surviving cells. Dentin seems to be more compatible with osteoblastslike cells than fibroblast. FDBA has shown the least favorable results. Cellular attachment for both the cell lines can be appreciated on SEM images. Conclusion: Demineralized particulate dentin has reported considerable percentage of cell viability making it a reasonable option for bone grafting substitute.

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