Evaluation of Human Periodontal Ligament and Osteosarcoma Cell Attachment and Viability on Particulate Bone and Dentin Allografts

2020 ◽  
pp. 1-6
Keyword(s):  
Particle Size ◽  
Cell Lines ◽  
Bone Grafting ◽  
Periodontal Ligament ◽  
Cell Attachment ◽  
Bone Allograft ◽  
Osteosarcoma Cell ◽  
Cellular Attachment ◽  
Freeze Dried ◽  
Demineralized Dentin

Purpose: Various bone grafting substitutes have been used in the periodontics for bone regeneration which include autografts, allografts, xenografts and alloplasts. Autogenous particulate dentin has been used successfully as a bone grafting substitute. The aim of present study was to evaluate the effect of demineralized and mineralized freeze-dried bone allograft and particulate dentin on osteoblasts-like cells and human periodontal fibroblasts. Materials and methods: Demineralized freeze-dried bone allograft (DFDBA) and freeze-dried bone allograft (FDBA) and ground dentin was used in the study. Particulate dentin was divided into four groups according to the size of the particles and demineralization - small dentin (particle size less than 200 µm), small dentin demineralized, large dentin (particle size 250-1200 µm), large dentin demineralized. Effect of all the specimens was checked on osteoblast-like cells (MG63) and human periodontal ligament cell lines. Percentage of surviving cells was measured using colorimetric MTT assay spectrophotometrically on 7th and 14th day of the cell culture. Scanning electron microscopy (SEM) was used to check the cellular attachment. Results: Demineralized dentin matrix has shown significantly enhanced viable cell percentage for both the cell lines. DFDBA and demineralized dentin has reported comparable percentage of surviving cells. Dentin seems to be more compatible with osteoblastslike cells than fibroblast. FDBA has shown the least favorable results. Cellular attachment for both the cell lines can be appreciated on SEM images. Conclusion: Demineralized particulate dentin has reported considerable percentage of cell viability making it a reasonable option for bone grafting substitute.

Particle size influence in an impaction bone grafting model. Comparison of fresh-frozen and freeze-dried allografts

2009 ◽  
Vol 42 (14) ◽  
pp. 2238-2242 ◽  
Author(s):  
Olivier Cornu ◽  
Thomas Schubert ◽  
Xavier Libouton ◽  
Olivier Manil ◽  
Bernard Godts ◽  
...  
Keyword(s):  
Particle Size ◽  
Bone Grafting ◽  
Model Comparison ◽  
Impaction Bone Grafting ◽  
Freeze Dried ◽  
Fresh Frozen

scholarly journals Clinical and radiographic evaluation of platelet-rich fibrin as an adjunct to bone grafting demineralized freeze-dried bone allograft in intrabony defects

2020 ◽  
Vol 24 (1) ◽  
pp. 60 ◽  
Author(s):  
Abhinav Atchuta ◽  
JagadishReddy Gooty ◽  
VikramReddy Guntakandla ◽  
SunilKumar Palakuru ◽  
Satyanarayana Durvasula ◽  
...  
Keyword(s):  
Bone Grafting ◽  
Bone Allograft ◽  
Radiographic Evaluation ◽  
Intrabony Defects ◽  
Platelet Rich Fibrin ◽  
Freeze Dried

scholarly journals Bone Grafts in Periodontics-A Review

2015 ◽  
Vol 7 (2) ◽  
pp. 64-70
Author(s):  
VT Pramod
Keyword(s):  
Bone Formation ◽  
Bone Grafting ◽  
Bone Allograft ◽  
Bone Grafts ◽  
Periodontal Health ◽  
Periodontal Tissues ◽  
Freeze Dried ◽  
Bone Grafting Materials ◽  
Grafting Materials ◽  
Induce Bone Formation

Abstract The ultimate goal of periodontal therapy should not be limited to the establishment and maintenance of periodontal health. The potential for regeneration of the hard and soft periodontal tissues lost to disease should be considered. Of all the bone grafting materials being developed, the demineralized freeze dried bone allograft (DFDBA) has been used as a substitute for bone graft for more than four decades. The basis for the use of any bone grafting material is to induce bone formation. In this article various bone grafts and biomaterials used are reviewed. How to cite this article Tatuskar P, Prakash S. Bone Grafts in Periodontics -A Review. CODS J Dent 2015;7: 64-70.

scholarly journals Transformation-associated alterations in interactions between pre-B cells and fibronectin

Blood ◽  
1990 ◽  
Vol 76 (11) ◽  
pp. 2311-2320 ◽  
Author(s):  
FM Lemoine ◽  
S Dedhar ◽  
GM Lima ◽  
CJ Eaves
Keyword(s):  
B Cells ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Cell Attachment ◽  
Attachment Site ◽  
Receptor Antibodies

Abstract Marrow stromal elements produce as yet uncharacterized soluble growth factors that can stimulate the proliferation of murine pre-B cells, although close contact between these two cell types appears to ensure a better pre-B cell response. We have now shown that freshly isolated normal pre-B cells (ie, the B220+, surface mu- fraction of adult mouse bone marrow) adhere to fibronectin (FN) via an RGD cell-attachment site, as shown in a serum-free adherence assay, and they lose this functional ability on differentiation in vivo into B cells (ie, the B220+, surface mu+ fraction). Similarly, cells from an immortalized but stromal cell-dependent and nontumorigenic murine pre-B cell line originally derived from a Whitlock-Witte culture were also found to adhere to fibronectin (FN) via an RGD cell-attachment site. Moreover, in the presence of anti-FN receptor antibodies, the ability of this immortalized pre-B cell line to proliferate when co-cultured with a supportive stromal cell line (M2–10B4 cells) was markedly reduced (down to 30% of control). This suggests that pre-B cell attachment to FN on stromal cells may be an important component of the mechanism by which stromal cells stimulate normal pre-B cell proliferation and one that is no longer operative to control their more differentiated progeny. Two differently transformed pre-B cell lines, both of which are autocrine, stromal-independent, tumorigenic in vivo, and partially or completely differentiation-arrested at a very early stage of pre-B cell development, did not bind to FN. In addition, anti-FN receptor antibodies were much less effective in diminishing the ability of these tumorigenic pre-B cells to respond to M2–10B4 cell stimulation, which could still be demonstrated when the tumorigenic pre-B cells were co- cultured with M2–10B4 cells at a sufficiently low cell density. Analysis of cell surface molecules immunoprecipitated from both the nontumorigenic and tumorigenic pre-B cell lines by an anti-FN receptor antibody showed an increase in very late antigen (VLA) alpha chain(s) in both tumorigenic pre-B cell lines and a decrease in the beta 1 chain in one. Interestingly, all of the pre-B cell lines expressed similar amounts of messenger RNA for the beta 1 chain of the FN receptor. These results suggest that alteration of FN receptor expression on pre-B cells may represent a mechanism contributing to the outgrowth of leukemic pre-B cells with an autocrine phenotype and capable of stromal cell-independent, autonomous growth.

scholarly journals Anti-Metastatic and Anti-Angiogenic Effects of Curcumin Analog DK1 on Human Osteosarcoma Cells In Vitro

2021 ◽  
Vol 14 (6) ◽  
pp. 532
Author(s):  
Muhammad Nazirul Mubin Aziz ◽  
Nurul Fattin Che Rahim ◽  
Yazmin Hussin ◽  
Swee Keong Yeap ◽  
Mas Jaffri Masarudin ◽  
...  
Keyword(s):  
Cell Lines ◽  
Safety Profile ◽  
Quantitative Polymerase Chain Reaction ◽  
Tube Formation ◽  
Osteosarcoma Cell ◽  
Curcumin Analog ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Human Osteosarcoma Cell

Osteosarcoma (OS) is a life-threatening malignant bone tumor associated with poor prognosis among children. The survival rate of the patient is still arguably low even with intensive treatment provided, plus with the inherent side effects from the chemotherapy, which gives more unfavorable outcomes. Hence, the search for potent anti-osteosarcoma agent with promising safety profile is still on going. Natural occurring substance like curcumin has gained a lot of attention due to its splendid safety profile as well as it pharmacological advantages such as anti-metastasis and anti-angiogenesis. However, natural curcumin was widely known for its poor cellular uptake, which undermines all potential that it possesses. This prompted the development of synthetically synthesized curcuminoid analog, known as (Z)-3-hydroxy-1-(2-hydroxyphenyl)-3-phenylprop-2- en-1-one (DK1). In this present study, in vitro scratch assay, transwell migration/invasion assay, HUVEC tube formation assay, and ex vivo rat aortic ring assays were performed in order to investigate the anti-metastatic and anti-angiogenic potential of DK1. For further comprehension of DK1 mechanism on human osteosarcoma cell lines, microarray gene expression analysis, quantitative polymerase chain reaction (qPCR), and proteome profiler were adopted, providing valuable forecast from the expression of important genes and proteins related to metastasis and angiogenesis. Based on the data gathered from the bioassays, DK1 was able to inhibit the metastasis and angiogenesis of human osteosarcoma cell lines by significantly reducing the cell motility, number of migrated and invaded cells as well as the tube formation and micro-vessels sprouting. Additionally, DK1 also has significantly regulated several cancer pathways involved in OS proliferation, metastasis, and angiogenesis such as PI3K/Akt and NF-κB in both U-2 OS and MG-63. Regulation of PI3K/Akt caused up-regulation of genes related to metastasis inhibition, namely, PTEN, FOXO, PLK3, and GADD45A. Meanwhile, NF-κB pathway was regulated by mitigating the expression of NF-κB activator such as IKBKB and IKBKE in MG-63, whilst up-regulating the expression of NF-κB inhibitors such as NFKBIA and NFKBIE in U-2 OS. Finally, DK1 also has successfully hindered the metastatic and angiogenic capability of OS cell lines by down-regulating the expression of pro-metastatic genes and proteins like MMP3, COL11A1, FGF1, Endoglin, uPA, and IGFBP2 in U-2 OS. Whilst for MG-63, the significantly down-regulated oncogenes were Serpin E1, AKT2, VEGF, uPA, PD-ECGF, and Endoglin. These results suggest that curcumin analog DK1 may serve as a potential new anti-osteosarcoma agent due to its anti-metastatic and anti-angiogenic attributes.

scholarly journals Volumetric changes in edentulous alveolar ridge sites utilizing guided bone regeneration and a custom titanium ridge augmentation matrix (CTRAM): a case series study

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Craig E. Hofferber ◽  
J. Cameron Beck ◽  
Peter C. Liacouras ◽  
Jeffrey R. Wessel ◽  
Thu P. Getka
Keyword(s):  
Case Series ◽  
Bone Allograft ◽  
Volumetric Analysis ◽  
Collagen Membrane ◽  
Ridge Augmentation ◽  
Implant Placement ◽  
Case Series Study ◽  
Freeze Dried ◽  
Clinical Measurements ◽  
Bone Gain

Abstract Background The purpose of this study was to evaluate the volumetric changes in partially edentulous alveolar ridges augmented with customized titanium ridge augmentation matrices (CTRAM), freeze-dried bone allograft, and a resorbable collagen membrane. Methods A pre-surgical cone beam computed tomography (CBCT) scan was obtained for CTRAM design/fabrication and to evaluate pre-surgical ridge dimensions. Ridge augmentation surgery using CTRAM, freeze-dried bone allograft, and a resorbable collagen membrane was performed at each deficient site. Clinical measurements of the area of augmentation were made at the time of CTRAM placement and re-entry, and a 2nd CBCT scan 7 months after graft placement was used for volumetric analysis. Locations of each CTRAM in situ were also compared to their planned positions. Re-entry surgery and implant placement was performed 8 months after CTRAM placement. Results Nine subjects were treated with CTRAM and freeze-dried bone allograft. Four out of the nine patients enrolled (44.4%) experienced premature CTRAM exposure during healing, and in two of these cases, CTRAM were removed early. Early exposure did not result in total graft failure in any case. Mean volumetric bone gain was 85.5 ± 30.9% of planned augmentation volume (61.3 ± 33.6% in subjects with premature CTRAM exposure vs. 104.9% for subjects without premature exposure, p = 0.03). Mean horizontal augmentation (measured clinically) was 3.02 mm, and vertical augmentation 2.86 mm. Mean surgical positional deviation of CTRAM from the planned location was 1.09 mm. Conclusion The use of CTRAM in conjunction with bone graft and a collagen membrane resulted in vertical and horizontal bone gain suitable for implant placement.

scholarly journals Correction to: Afatinib is active in osteosarcoma cell lines

2020 ◽  
Vol 146 (10) ◽  
pp. 2719-2719
Author(s):  
Marlid Cruz-Ramos ◽  
Yessica Zamudio-Cuevas ◽  
Daniel Medina-Luna ◽  
Karina Martínez-Flores ◽  
Gabriela Martínez-Nava ◽  
...  
Keyword(s):  
Cell Lines ◽  
Osteosarcoma Cell

scholarly journals LncRNA SNHG15 contributes to doxorubicin resistance of osteosarcoma cells through targeting the miR-381-3p/GFRA1 axis

2020 ◽  
Vol 15 (1) ◽  
pp. 871-883
Author(s):  
Jinshan Zhang ◽  
Dan Rao ◽  
Haibo Ma ◽  
Defeng Kong ◽  
Xiaoming Xu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Noncoding Rna ◽  
Xenograft Model ◽  
Inhibitory Effect ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Osteosarcoma Cell ◽  
Osteosarcoma Cells

AbstractBackgroundOsteosarcoma is a common primary malignant bone cancer. Long noncoding RNA small nucleolar RNA host gene 15 (SNHG15) has been reported to play an oncogenic role in many cancers. Nevertheless, the role of SNHG15 in the doxorubicin (DXR) resistance of osteosarcoma cells has not been fully addressed.MethodsCell Counting Kit-8 assay was conducted to measure the half-maximal inhibitory concentration value of DXR in osteosarcoma cells. Western blotting was carried out to examine the levels of autophagy-related proteins and GDNF family receptor alpha-1 (GFRA1). Quantitative reverse transcription-polymerase chain reaction was performed to determine the levels of SNHG15, miR-381-3p, and GFRA1. The proliferation of osteosarcoma cells was measured by MTT assay. The binding sites between miR-381-3p and SNHG15 or GFRA1 were predicted by Starbase bioinformatics software, and the interaction was confirmed by dual-luciferase reporter assay. Murine xenograft model was established to validate the function of SNHG15 in vivo.ResultsAutophagy inhibitor 3-methyladenine sensitized DXR-resistant osteosarcoma cell lines to DXR. SNHG15 was upregulated in DXR-resistant osteosarcoma tissues and cell lines. SNHG15 knockdown inhibited the proliferation, DXR resistance, and autophagy of osteosarcoma cells. MiR-381-3p was a direct target of SNHG15, and GFRA1 bound to miR-381-3p in osteosarcoma cells. SNHG15 contributed to DXR resistance through the miR-381-3p/GFRA1 axis in vitro. SNHG15 depletion contributed to the inhibitory effect of DXR on osteosarcoma tumor growth through the miR-381-3p/GFRA1 axis in vivo.ConclusionsSNHG15 enhanced the DXR resistance of osteosarcoma cells through elevating the autophagy via targeting the miR-381-3p/GFRA1 axis. Restoration of miR-381-3p expression might be an underlying therapeutic strategy to overcome the DXR resistance of osteosarcoma.

scholarly journals Characterization of dormancy in osteosarcoma cell lines cultured in 3D

Bone Reports ◽  
2021 ◽  
Vol 14 ◽  
pp. 100898
Author(s):  
Camille Jubelin ◽  
Denis Cochonneau ◽  
Javier Munoz-Garcia ◽  
Emilie Moranton ◽  
Marie-Françoise Heymann ◽  
...  
Keyword(s):  
Cell Lines ◽  
Osteosarcoma Cell
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