The Effect of Cryoirrigation and Cryopreserved Placenta Extract On The Content of Nitrogen Monoxide In The Gastric Mucosa In Rats With Diclofenac Sodium-Induced Gastropathy

Background: Diclofenac sodium is a non-steroidal anti-inflammatory drug with reportedly undesirable harmful side effects including blood disorders, abdominal pain, ulceration and bleeding of the stomach or intestine. Objective:The present study was conducted to investigate the effect of diclofenac sodium on erythropoiesis, on stomach and liver under different conditions. Method: This experimental study on animal model was conducted in the Department of Physiology, Bangladesh Agricultural University, Mymensingh, between July and August 2007. A total of 45 Swiss Albino mice were randomly assigned into five equal groups (n=9) and they all were fed with standard broiler pellet (25 gm/mice/day) throughout the experimental period of 40 days. Keeping 1 group as control(groupA), 4 groups were treated with diclofenac sodium @ 3 mg/kg body weight (bwt) orally in empty (group B)or full stomach with (group c) or without vitamin B12 (group D) @ 10 μg/Kg body weight through intramuscular route. Total erythrocyte count (TEC) and Hb% was determined to assess erythropoiesis. Macroscopic and microscopic changes of liver and stomach were studied by standard laboratory method. Data were analyzed statistically by ANOVA and un paired t test. Result: Oral administration of this drug significantly (p<0.01) decreased Total Erythrocyte Count(TEC) and Hb in the animals treated with diclofenac sodium in empty stomach and in the animals treated with diclofenac sodium plus vitamin B12 in empty stomach. All the treated mice showed slight to moderate congestion of the gastric mucosa and liver. Conclusion: Intake of diclofenac sodium in empty stomach may produce harmful side effect on stomach and liver. Key words: Stomach; Mice; Diclofenac sodium. DOI: 10.3329/jbsp.v5i2.6783J Bangladesh Soc Physiol. 2010 December; 5(2): 89-93

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Ultrastructure Research of the Gastritis from Returning Bile

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158558 ◽

1990 ◽

Vol 48 (3) ◽

pp. 202-203

Author(s):

Shaopeng Hu ◽

Jianhua Wang ◽

Zhen Li ◽

Huei Chen ◽

Fei Cu ◽

...

Keyword(s):

Electron Microscope ◽

Gastric Mucosa ◽

Scanning Electron Microscope ◽

Lamina Propria ◽

Transmission Electron Microscope ◽

Ultrastructural Change ◽

Common Disease ◽

Epithelial Surface ◽

Transmission Electron ◽

Scanning Electron

Gastritis from returning bile is a common disease, but the reason for the disease is not clear. As the pathologic ultrastructure research progresses, it has drawn attention to the ultrastructural change of cells in gastric mucosa by clinical workers. We observed gastric mucosa tissues of 15 patients suffering from gastritis with a transmission electron microscope (TEM) and a scanning electron microscope (SEM). It is the first report in China that fungus exists in the lamina propria of gastric mucosa tissue. The result is as follows.The gastric mucosa tissues of 15 patients suffering from gastritis were acquired by stomachoscopy. Both TEM and SEM specimens were prepared by the usual methods. Under the TEM, the epithelial surface became higher and larger. Mitochondria of the cells were swollen and cristae were disrupted. There were vacuoles in the cells. The nucleus showed disorder, heterochromatin became darker, and nucleolae could be observed.

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Sem Observations in Gastric Mucosa Exposed to Progressive Enzymatic Etching

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002531 ◽

1980 ◽

Vol 38 ◽

pp. 570-571

Author(s):

C.A.E. Lemmi ◽

D. Booth ◽

G.E. Adomian

Keyword(s):

Gastric Mucosa ◽

Critical Point ◽

Proteolytic Activity ◽

Etching Process ◽

Cellular Types

In order to enrich populations of homogeneous cellular types we dissociated gastric mucosa by enzymatic techniques. In addition, we used SEM to monitor the progressive etching of the mucosa. Two enzymes were tested: collagenase III with minimum proteolytic activity and Pronase with broader proteolytic effects. The gastric mucosa was exposed to the effect of the enzymes using everted stomach preparations. In this way the digestive action occured progressively from the lumen of the stomach toward the base of the glands. This “etching” process could be monitored conveniently by SEM. After incubation for periods varying from 30 to 210 minutes the tissues were stretched on dental wax, fixed in 2 % glutaralheyde, post-fixed in osmium, dehydrated, critical point dryed and coated with gold. A model MSM-5 “Mini-SEM” was used for observation. Gentle uncurling of the preparation before coating with gold produced fractures which revealed the structure of the gastric glandsin more detail.

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