TNFSF/TNFRSF cytokine gene expression in sickle cell anemia: Up-regulated TNF-like cytokine 1A (TL1A) and its decoy receptor (DcR3) in peripheral blood mononuclear cells and plasma

Cytokine ◽  
2019 ◽  
Vol 123 ◽  
pp. 154744
Author(s):  
Surinder Safaya ◽  
Mohammed Alfarhan ◽  
Ahmed Sulaiman ◽  
Abdulla Alsulaiman ◽  
Amein Al-Ali
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Sickle Cell Anemia ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Peripheral Blood Mononuclear ◽  
Decoy Receptor ◽  
Blood Mononuclear Cells

scholarly journals TLR-Mediated Cytokine Gene Expression in Chicken Peripheral Blood Mononuclear Cells as a Measure to Characterize Immunobiotics

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 195
Author(s):  
Anna Slawinska ◽  
Aleksandra Dunislawska ◽  
Arkadiusz Plowiec ◽  
José Gonçalves ◽  
Maria Siwek
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Peripheral Blood Mononuclear ◽  
Inflammatory Genes ◽  
Blood Mononuclear Cells ◽  
Time Point

Immunobiotics are probiotics that promote intestinal health by modulating immune responses. Immunobiotics are recognized by Toll-like receptors (TLRs) and activate cytokine gene expression. This study aimed to characterize cytokine gene expression in the chicken peripheral blood mononuclear cells (PBMC) stimulated with purified TLR ligands and live probiotics. PBMC were isolated from the whole blood. PBMC were stimulated with: lipopolysaccharide (LPS), CpG ODN, Pam3CSK4, Zymosan, galactooligosaccharides (GOS), Lactococcuslactis subsp. cremoris (L. lactis), and Saccharomyces cerevisiae at 42.5 °C and 5% CO2 for 3 h, 6 h, and 9 h. After each time-point, PBMC were harvested for RNA isolation. Relative gene expression was analyzed with RT-qPCR for cytokine genes (IL-1β, IL-2, IL-3, IL-4, IL-6, IL-8, IL-10, IL-12p40, and IFN-ɣ) and reference genes (ACTB and G6PDH). Genes were clustered into pro-inflammatory genes, Th1/Th2 genes, and Th1-regulators. The gene expression differed between treatments in IL1-β, IL-6, IL-8, IL-10, and IL-12p40 (p < 0.001). The genes IL-1β, IL-6, and IL-8 had the highest fold change of mRNA expression at 3 h in response to TLR ligands. L. lactis up-regulated the pro-inflammatory genes at the 6 h time-point. L. lactis did not activate the anti-inflammatory IL-10 gene, but activated IL-12p40 at 6 h. Hereby, L. lactis was proven to exert immunostimulatory properties in PBMC.

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