Characterization of the cathepsin D in Procambarus clarkii and its biological role in innate immune responses

2020 ◽  
Vol 111 ◽  
pp. 103766
Author(s):  
Xiao-Min Yu ◽  
Jia-Le Chen ◽  
Muhammad Nadeem Abbas ◽  
Isma Gul ◽  
Saima Kausar ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cathepsin D ◽  
Procambarus Clarkii ◽  
Innate Immune ◽  
Biological Role ◽  
Innate Immune Responses

scholarly journals Characterization of a long-term mouse primary liver 3D tissue model recapitulating innate-immune responses and drug-induced liver toxicity

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0235745 ◽  
Author(s):  
Ramona Nudischer ◽  
Kasper Renggli ◽  
Andreas Hierlemann ◽  
Adrian B. Roth ◽  
Cristina Bertinetti-Lapatki
Keyword(s):  
Immune Responses ◽  
Liver Toxicity ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Drug Induced ◽  
Tissue Model

scholarly journals Caspase-8 is activated by cathepsin D initiating neutrophil apoptosis during the resolution of inflammation

2008 ◽  
Vol 205 (3) ◽  
pp. 685-698 ◽  
Author(s):  
Sébastien Conus ◽  
Remo Perozzo ◽  
Thomas Reinheckel ◽  
Christoph Peters ◽  
Leonardo Scapozza ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cathepsin D ◽  
Defense Mechanisms ◽  
Inflammatory Responses ◽  
Innate Immune ◽  
Caspase 8 ◽  
Neutrophil Apoptosis ◽  
Dependent Manner ◽  
Innate Immune Responses ◽  
Inflammatory Conditions

In the resolution of inflammatory responses, neutrophils rapidly undergo apoptosis. We describe a new proapoptotic pathway in which cathepsin D directly activates caspase-8. Cathepsin D is released from azurophilic granules in neutrophils in a caspase-independent but reactive oxygen species–dependent manner. Under inflammatory conditions, the translocation of cathepsin D in the cytosol is blocked. Pharmacological or genetic inhibition of cathepsin D resulted in delayed caspase activation and reduced neutrophil apoptosis. Cathepsin D deficiency or lack of its translocation in the cytosol prolongs innate immune responses in experimental bacterial infection and in septic shock. Thus, we identified a new function of azurophilic granules that is in addition to their role in bacterial defense mechanisms: to regulate the life span of neutrophils and, therefore, the duration of innate immune responses through the release of cathepsin D.

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