Effects of silica and zinc oxide doping on mechanical and biological properties of 3D printed tricalcium phosphate tissue engineering scaffolds

Gelatin has excellent biological properties, but its poor physical properties are a major obstacle to its use as a biomaterial ink. These disadvantages not only worsen the printability of gelatin biomaterial ink, but also reduce the dimensional stability of its 3D scaffolds and limit its application in the tissue engineering field. Herein, biodegradable suture fibers were added into a gelatin biomaterial ink to improve the printability, mechanical strength, and dimensional stability of the 3D printed scaffolds. The suture fiber reinforced gelatin 3D scaffolds were fabricated using the thermo-responsive properties of gelatin under optimized 3D printing conditions (−10 °C cryogenic plate, 40–80 kPa pneumatic pressure, and 9 mm/s printing speed), and were crosslinked using EDC/NHS to maintain their 3D structures. Scanning electron microscopy images revealed that the morphologies of the 3D printed scaffolds maintained their 3D structure after crosslinking. The addition of 0.5% (w/v) of suture fibers increased the printing accuracy of the 3D printed scaffolds to 97%. The suture fibers also increased the mechanical strength of the 3D printed scaffolds by up to 6-fold, and the degradation rate could be controlled by the suture fiber content. In in vitro cell studies, DNA assay results showed that human dermal fibroblasts’ proliferation rate of a 3D printed scaffold containing 0.5% suture fiber was 10% higher than that of a 3D printed scaffold without suture fibers after 14 days of culture. Interestingly, the supplement of suture fibers into gelatin biomaterial ink was able to minimize the cell-mediated contraction of the cell cultured 3D scaffolds over the cell culture period. These results show that advanced biomaterial inks can be developed by supplementing biodegradable fibers to improve the poor physical properties of natural polymer-based biomaterial inks.

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Fabrication of scalable and structured tissue engineering scaffolds using water dissolvable sacrificial 3D printed moulds

Materials Science and Engineering C ◽

10.1016/j.msec.2015.06.002 ◽

2015 ◽

Vol 55 ◽

pp. 569-578 ◽

Cited By ~ 98

Author(s):

Soumyaranjan Mohanty ◽

Layla Bashir Larsen ◽

Jon Trifol ◽

Peter Szabo ◽

Harsha Vardhan Reddy Burri ◽

...

Keyword(s):

Tissue Engineering ◽

Tissue Engineering Scaffolds ◽

3D Printed

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Evaluation of the effects of β-tricalcium phosphate on physical, mechanical and biological properties of Poly (3-hydroxybutyrate)/chitosan electrospun scaffold for cartilage tissue engineering applications

Materials Technology ◽

10.1080/10667857.2019.1611053 ◽

2019 ◽

Vol 34 (10) ◽

pp. 615-625 ◽

Cited By ~ 9

Author(s):

Sima Keikhaei ◽

Zahra Mohammadalizadeh ◽

Saeed Karbasi ◽

Ali Salimi

Keyword(s):

Tissue Engineering ◽

Tricalcium Phosphate ◽

Cartilage Tissue Engineering ◽

Biological Properties ◽

Cartilage Tissue ◽

Electrospun Scaffold ◽

Engineering Applications

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Marine Algae Polysaccharides as Basis for Wound Dressings, Drug Delivery, and Tissue Engineering: A Review

Journal of Marine Science and Engineering ◽

10.3390/jmse8070481 ◽

2020 ◽

Vol 8 (7) ◽

pp. 481 ◽

Cited By ~ 6

Author(s):

Tatyana A. Kuznetsova ◽

Boris G. Andryukov ◽

Natalia N. Besednova ◽

Tatyana S. Zaporozhets ◽

Andrey V. Kalinin

Keyword(s):

Drug Delivery ◽

Tissue Engineering ◽

Regenerative Medicine ◽

Wound Dressing ◽

Biological Properties ◽

Wound Dressings ◽

Tissue Engineering Scaffolds ◽

Treatment Of Wounds ◽

New Generation ◽

Dressing Materials

The present review considers the physicochemical and biological properties of polysaccharides (PS) from brown, red, and green algae (alginates, fucoidans, carrageenans, and ulvans) used in the latest technologies of regenerative medicine (tissue engineering, modulation of the drug delivery system, and the design of wound dressing materials). Information on various types of modern biodegradable and biocompatible PS-based wound dressings (membranes, foams, hydrogels, nanofibers, and sponges) is provided; the results of experimental and clinical trials of some dressing materials in the treatment of wounds of various origins are analyzed. Special attention is paid to the ability of PS to form hydrogels, as hydrogel dressings meet the basic requirements set out for a perfect wound dressing. The current trends in the development of new-generation PS-based materials for designing drug delivery systems and various tissue-engineering scaffolds, which makes it possible to create human-specific tissues and develop target-oriented and personalized regenerative medicine products, are also discussed.

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3D Printed porous tissue engineering scaffolds with the self-folding ability and controlled release of growth factor

MRS Communications ◽

10.1557/mrc.2020.65 ◽

2020 ◽

Vol 10 (4) ◽

pp. 579-586

Author(s):

Jiahui Lai ◽

Junzhi Li ◽

Min Wang

Keyword(s):

Tissue Engineering ◽

Controlled Release ◽

Growth Factor ◽

The Self ◽

Tissue Engineering Scaffolds ◽

3D Printed

Abstract

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Enhancing X-ray Attenuation of 3D Printed Gelatin Methacrylate (GelMA) Hydrogels Utilizing Gold Nanoparticles for Bone Tissue Engineering Applications

Polymers ◽

10.3390/polym11020367 ◽

2019 ◽

Vol 11 (2) ◽

pp. 367 ◽

Cited By ~ 9

Author(s):

Nehar Celikkin ◽

Simone Mastrogiacomo ◽

X. Walboomers ◽

Wojciech Swieszkowski

Keyword(s):

Tissue Engineering ◽

Gold Nanoparticles ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biological Properties ◽

Proof Of Concept ◽

New Bone Formation ◽

X Ray ◽

3D Printed ◽

Low Toxicity

Bone tissue engineering is a rapidly growing field which is currently progressing toward clinical applications. Effective imaging methods for longitudinal studies are critical to evaluating the new bone formation and the fate of the scaffolds. Computed tomography (CT) is a prevailing technique employed to investigate hard tissue scaffolds; however, the CT signal becomes weak in mainly-water containing materials, which hinders the use of CT for hydrogels-based materials. Nevertheless, hydrogels such as gelatin methacrylate (GelMA) are widely used for tissue regeneration due to their optimal biological properties and their ability to induce extracellular matrix formation. To date, gold nanoparticles (AuNPs) have been suggested as promising contrast agents, due to their high X-ray attenuation, biocompatibility, and low toxicity. In this study, the effects of different sizes and concentrations of AuNPs on the mechanical properties and the cytocompatibility of the bulk GelMA-AuNPs scaffolds were evaluated. Furthermore, the enhancement of CT contrast with the cytocompatible size and concentration of AuNPs were investigated. 3D printed GelMA and GelMA-AuNPs scaffolds were obtained and assessed for the osteogenic differentiation of mesenchymal stem cells (MSC). Lastly, 3D printed GelMA and GelMA-AuNPs scaffolds were scanned in a bone defect utilizing µCT as the proof of concept that the GelMA-AuNPs are good candidates for bone tissue engineering with enhanced visibility for µCT imaging.

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