scholarly journals Role of Actin Cytoskeleton in Brassinosteroid Signaling and in Its Integration with the Auxin Response in Plants

2012 ◽  
Vol 22 (6) ◽  
pp. 1275-1285 ◽  
Author(s):  
Mónica Lanza ◽  
Berenice Garcia-Ponce ◽  
Gabriel Castrillo ◽  
Pablo Catarecha ◽  
Michael Sauer ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Auxin Response ◽  
Brassinosteroid Signaling

Membrane Homeostasis: The Role of Actin Cytoskeleton

2021 ◽  
Vol 101 (1) ◽  
pp. 81-95
Author(s):  
Arikta Biswas ◽  
Rinku Kumar ◽  
Bidisha Sinha
Keyword(s):  
Actin Cytoskeleton

scholarly journals Interaction of the Tobacco Mosaic Virus Replicase Protein with the Aux/IAA Protein PAP1/IAA26 Is Associated with Disease Development

2005 ◽  
Vol 79 (4) ◽  
pp. 2549-2558 ◽  
Author(s):  
Meenu S. Padmanabhan ◽  
Sameer P. Goregaoker ◽  
Sheetal Golem ◽  
Haiymanot Shiferaw ◽  
James N. Culver
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Protein S ◽  
Helicase Domain ◽  
Auxin Response ◽  
Disease Symptoms ◽  
Developmental Abnormalities ◽  
Replicase Protein ◽  
Transcriptional Alterations

ABSTRACT Virus-infected plants often display developmental abnormalities that include stunting, leaf curling, and the loss of apical dominance. In this study, the helicase domain of the Tobacco mosaic virus (TMV) 126- and/or 183-kDa replicase protein(s) was found to interact with the Arabidopsis Aux/IAA protein PAP1 (also named IAA26), a putative regulator of auxin response genes involved in plant development. To investigate the role of this interaction in the display of symptoms, a TMV mutant defective in the PAP1 interaction was identified. This mutant replicated and moved normally in Arabidopsis but induced attenuated developmental symptoms. Additionally, transgenic plants in which the accumulation of PAP1 mRNA was silenced exhibit symptoms like those of virus-infected plants. In uninfected tissues, ectopically expressed PAP1 accumulated and localized to the nucleus. However, in TMV-infected tissues, PAP1 failed to accumulate to significant levels and did not localize to the nucleus, suggesting that interaction with the TMV replicase protein disrupts PAP1 localization. The consequences of this interaction would affect PAP1's putative function as a transcriptional regulator of auxin response genes. This is supported by gene expression data indicating that ∼30% of the Arabidopsis genes displaying transcriptional alterations in response to TMV contain multiple auxin response promoter elements. Combined, these data indicate that the TMV replicase protein interferes with the plant's auxin response system to induce specific disease symptoms.

scholarly journals The role of the lens actin cytoskeleton in fiber cell elongation and differentiation

2006 ◽  
Vol 17 (6) ◽  
pp. 698-711 ◽  
Author(s):  
P. Vasantha Rao ◽  
Rupalatha Maddala
Keyword(s):  
Actin Cytoskeleton ◽  
Cell Elongation ◽  
Fiber Cell

The role of plant villin in the organization of the actin cytoskeleton, cytoplasmic streaming and the architecture of the transvacuolar strand in root hair cells of Hydrocharis

Planta ◽  
2000 ◽  
Vol 210 (5) ◽  
pp. 836-843 ◽  
Author(s):  
Motoki Tominaga ◽  
Etsuo Yokota ◽  
Luis Vidali ◽  
Seiji Sonobe ◽  
Peter K. Hepler ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Hair Cells ◽  
Root Hair ◽  
Cytoplasmic Streaming ◽  
Root Hair Cells

scholarly journals A Role of Cofilin/Destrin in Reorganization of Actin Cytoskeleton in Response to Stresses and Cell Stimuli.

1996 ◽  
Vol 21 (5) ◽  
pp. 421-424 ◽  
Author(s):  
Ichiro Yahara ◽  
Hiroyuki Aizawa ◽  
Kenji Moriyama ◽  
Kazuko Iida ◽  
Naoto Yonezawa ◽  
...  
Keyword(s):  
Actin Cytoskeleton

scholarly journals The Role of WAVE2 Signaling in Cancer

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1217
Author(s):  
Priyanka Shailendra Rana ◽  
Akram Alkrekshi ◽  
Wei Wang ◽  
Vesna Markovic ◽  
Khalid Sossey-Alaoui
Keyword(s):  
Actin Cytoskeleton ◽  
Cancer Cell ◽  
Cell Invasion ◽  
Critical Role ◽  
Therapeutic Strategies ◽  
Small Gtpases ◽  
Cancer Cell Invasion ◽  
Invasion And Metastasis ◽  
Regulatory Complex

The Wiskott–Aldrich syndrome protein (WASP) and WASP family verprolin-homologous protein (WAVE)—WAVE1, WAVE2 and WAVE3 regulate rapid reorganization of cortical actin filaments and have been shown to form a key link between small GTPases and the actin cytoskeleton. Upon receiving upstream signals from Rho-family GTPases, the WASP and WAVE family proteins play a significant role in polymerization of actin cytoskeleton through activation of actin-related protein 2/3 complex (Arp2/3). The Arp2/3 complex, once activated, forms actin-based membrane protrusions essential for cell migration and cancer cell invasion. Thus, by activation of Arp2/3 complex, the WAVE and WASP family proteins, as part of the WAVE regulatory complex (WRC), have been shown to play a critical role in cancer cell invasion and metastasis, drawing significant research interest over recent years. Several studies have highlighted the potential for targeting the genes encoding either part of or a complete protein from the WASP/WAVE family as therapeutic strategies for preventing the invasion and metastasis of cancer cells. WAVE2 is well documented to be associated with the pathogenesis of several human cancers, including lung, liver, pancreatic, prostate, colorectal and breast cancer, as well as other hematologic malignancies. This review focuses mainly on the role of WAVE2 in the development, invasion and metastasis of different types of cancer. This review also summarizes the molecular mechanisms that regulate the activity of WAVE2, as well as those oncogenic pathways that are regulated by WAVE2 to promote the cancer phenotype. Finally, we discuss potential therapeutic strategies that target WAVE2 or the WAVE regulatory complex, aimed at preventing or inhibiting cancer invasion and metastasis.

scholarly journals Bundling up the Role of the Actin Cytoskeleton in Primary Root Growth

2021 ◽  
Vol 12 ◽  
Author(s):  
Judith García-González ◽  
Kasper van Gelderen
Keyword(s):  
Root Growth ◽  
Actin Cytoskeleton ◽  
Cell Elongation ◽  
Feedback Loop ◽  
Primary Root ◽  
Actin Binding ◽  
Actin Network ◽  
Actin Organization ◽  
Primary Root Growth

Primary root growth is required by the plant to anchor in the soil and reach out for nutrients and water, while dealing with obstacles. Efficient root elongation and bending depends upon the coordinated action of environmental sensing, signal transduction, and growth responses. The actin cytoskeleton is a highly plastic network that constitutes a point of integration for environmental stimuli and hormonal pathways. In this review, we present a detailed compilation highlighting the importance of the actin cytoskeleton during primary root growth and we describe how actin-binding proteins, plant hormones, and actin-disrupting drugs affect root growth and root actin. We also discuss the feedback loop between actin and root responses to light and gravity. Actin affects cell division and elongation through the control of its own organization. We remark upon the importance of longitudinally oriented actin bundles as a hallmark of cell elongation as well as the role of the actin cytoskeleton in protein trafficking and vacuolar reshaping during this process. The actin network is shaped by a plethora of actin-binding proteins; however, there is still a large gap in connecting the molecular function of these proteins with their developmental effects. Here, we summarize their function and known effects on primary root growth with a focus on their high level of specialization. Light and gravity are key factors that help us understand root growth directionality. The response of the root to gravity relies on hormonal, particularly auxin, homeostasis, and the actin cytoskeleton. Actin is necessary for the perception of the gravity stimulus via the repositioning of sedimenting statoliths, but it is also involved in mediating the growth response via the trafficking of auxin transporters and cell elongation. Furthermore, auxin and auxin analogs can affect the composition of the actin network, indicating a potential feedback loop. Light, in its turn, affects actin organization and hence, root growth, although its precise role remains largely unknown. Recently, fundamental studies with the latest techniques have given us more in-depth knowledge of the role and organization of actin in the coordination of root growth; however, there remains a lot to discover, especially in how actin organization helps cell shaping, and therefore root growth.

Role of actin cytoskeleton in podocytes

2020 ◽  
Author(s):  
Sanja Sever
Keyword(s):  
Actin Cytoskeleton

Actin filament organization is required for proper cAMP-dependent activation of CFTR

1999 ◽  
Vol 277 (6) ◽  
pp. C1160-C1169 ◽  
Author(s):  
Adriana G. Prat ◽  
C. Casey Cunningham ◽  
G. Robert Jackson ◽  
Steven C. Borkan ◽  
Yihan Wang ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Single Channel ◽  
Fold Increase ◽  
Transmembrane Conductance Regulator ◽  
Transmembrane Conductance ◽  
Whole Cell ◽  
Excised Patches ◽  
Cystic Fibrosis Transmembrane ◽  
Molecular Nature

Previous studies have indicated a role of the actin cytoskeleton in the regulation of the cystic fibrosis transmembrane conductance regulator (CFTR) ion channel. However, the exact molecular nature of this regulation is still largely unknown. In this report human epithelial CFTR was expressed in human melanoma cells genetically devoid of the filamin homologue actin-cross-linking protein ABP-280 [ABP(−)]. cAMP stimulation of ABP(−) cells or cells genetically rescued with ABP-280 cDNA [ABP(+)] was without effect on whole cell Cl− currents. In ABP(−) cells expressing CFTR, cAMP was also without effect on Cl− conductance. In contrast, cAMP induced a 10-fold increase in the diphenylamine-2-carboxylate (DPC)-sensitive whole cell Cl− currents of ABP(+)/CFTR(+) cells. Further, in cells expressing both CFTR and a truncated form of ABP-280 unable to cross-link actin filaments, cAMP was also without effect on CFTR activation. Dialysis of ABP-280 or filamin through the patch pipette, however, resulted in a DPC-inhibitable increase in the whole cell currents of ABP(−)/CFTR(+) cells. At the single-channel level, protein kinase A plus ATP activated single Cl−channels only in excised patches from ABP(+)/CFTR(+) cells. Furthermore, filamin alone also induced Cl− channel activity in excised patches of ABP(−)/CFTR(+) cells. The present data indicate that an organized actin cytoskeleton is required for cAMP-dependent activation of CFTR.

scholarly journals Genetic Evidence for an Indispensable Role of Somatic Embryogenesis Receptor Kinases in Brassinosteroid Signaling

PLoS Genetics ◽  
2012 ◽  
Vol 8 (1) ◽  
pp. e1002452 ◽  
Author(s):  
Xiaoping Gou ◽  
Hongju Yin ◽  
Kai He ◽  
Junbo Du ◽  
Jing Yi ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Evidence ◽  
Brassinosteroid Signaling ◽  
Receptor Kinases
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